Anti-AMPK Alpha 1/2 (phospho-T183/172) PRKAA1 Antibody

AMPK alpha 1 antibody

Boster Bio Anti-AMPK Alpha 1/2 (phospho-T183/172) PRKAA1 Antibody catalog # A00994T183. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.

Product Info Summary

SKU: A00994T183
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-AMPK Alpha 1/2 (phospho-T183/172) PRKAA1 Antibody

View all AMPK alpha 1 Antibodies

SKU/Catalog Number

A00994T183

Size

100ul

Form

Liquid

Description

Boster Bio Anti-AMPK Alpha 1/2 (phospho-T183/172) PRKAA1 Antibody catalog # A00994T183. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AMPK Alpha 1/2 (phospho-T183/172) PRKAA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00994T183)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human AMPKalpha1 around the phosphorylation site of S496.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00994T183 is reactive to PRKAA1 in Human, Mouse, Rat

Applications

A00994T183 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

64.009kDa

Background of AMPK alpha 1

4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Gingras AC, et al. (1998) Genes Dev 12(4): 502-513.
Brugarolas J, et al. (2004) Genes Dev 18(23): 2893-2904.
Kumar V, et al. (2000) EMBO J 19(5): 1087-1097.
Moody CA, et al. (2005) J Virol 79(9): 5499-5506.
Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95(4): 1432-1437.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000
IHC: 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For PRKAA1 (Source: Uniprot.org, NCBI)

Gene Name

PRKAA1

Full Name

5'-AMP-activated protein kinase catalytic subunit alpha-1

Weight

64.009kDa

Superfamily

protein kinase superfamily

Alternative Names

AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK alpha 1; AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain; AMPK subunit alpha-1; AMPK; AMPK1; AMPKa1; EC 2.7.11; EC 2.7.11.1; MGC33776; MGC57364; PRKAA1; protein kinase, AMP-activated, alpha 1 catalytic subunit PRKAA1 AMPK, AMPKa1 protein kinase AMP-activated catalytic subunit alpha 1 5-AMP-activated protein kinase catalytic subunit alpha-1|5-AMP-activated protein kinase, catalytic alpha-1 chain|ACACA kinase|AMP -activate kinase alpha 1 subunit|AMP-activated protein kinase, catalytic, alpha-1|AMPK alpha 1|AMPK subunit alpha-1|HMGCR kinase|acetyl-CoA carboxylase kinase|hydroxymethylglutaryl-CoA reductase kinase|protein kinase, AMP-activated, alpha 1 catalytic subunit|tau-protein kinase PRKAA1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PRKAA1, check out the PRKAA1 Infographic

PRKAA1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRKAA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A00994T183-100ul

100ul

$482

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