Click image to see more details
Product Info Summary
| SKU: | P00003-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-mTOR (S2481) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00003-1
BM4831 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-mTOR (S2481) Rabbit Monoclonal Antibody catalog # P00003-1. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-mTOR (S2481) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00003-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IEG-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-mTOR (S2481)
Reactive Species
P00003-1 is reactive to MTOR in Human, Mouse, Rat
Observed Molecular Weight
289 kDa
Calculated molecular weight
288.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00003-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: 293 cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-mTOR (S2481) expression in (1)293 cell lysate treated with LP; (2)293 cell lysate.
Specific Publications For Anti-Phospho-mTOR (S2481) Rabbit Monoclonal Antibody (P00003-1)
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4 Customer Q&As for Anti-Phospho-mTOR (S2481) Rabbit Monoclonal Antibody
Question
My boss were content with the WB result of your anti-Phospho-mTOR (S2481) Rabbit Monoclonal antibody. However we have observed positive staining in b-cell endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is MTOR supposed to be expressed?
R. Li
Verified customer
Asked: 2020-04-09
Answer
According to literature, b-cell does express MTOR. Generally MTOR expresses in endoplasmic reticulum membrane. Regarding which tissues have MTOR expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7809080
Brain, Pubmed ID: 8008069
Cerebellum, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-04-09
Question
We are currently using anti-Phospho-mTOR (S2481) Rabbit Monoclonal antibody P00003-1 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
The anti-Phospho-mTOR (S2481) Rabbit Monoclonal antibody (P00003-1) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-05
Question
We have been able to see staining in mouse cervix carcinoma. Do you have any suggestions? Is anti-Phospho-mTOR (S2481) Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2018-07-09
Answer
According to literature cervix carcinoma does express MTOR. According to Uniprot.org, MTOR is expressed in testis, brain, cerebellum, b-cell, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MTOR expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7809080
Brain, Pubmed ID: 8008069
Cerebellum, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2018-07-09
Question
A customer would like to know if P00003-1 (Abways: CY5996) and P00003 (Abways: CY6571) can be used on porcine. Could you help confirm with Abways?
Verified Customer
Verified customer
Asked: 2013-03-05
Answer
P00003-1 (Abways: CY5996) and P00003 (Abways: CY6571) haven't been woked on porcine sample. Abways is uncertain if they are suitable for porcine sample. The immnogen sequences of P00003-1 (Abways: CY5996) and P00003 (Abways: CY6571) are CPESIH-pS-FIGDGL and CSRTRTD-pS-YSAG respectively.
Boster Scientific Support
Answered: 2013-03-05
