Anti-APP/C99 Antibody Picoband™

Boster Bio Anti-APP/C99 Antibody Picoband™ catalog # A00081-3. Tested in IHC-P, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: A00081-3
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC-P, WB

Product Name

Anti-APP/C99 Antibody Picoband™

See all APP products

SKU/Catalog Number

A00081-3

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-APP/C99 Antibody Picoband™ catalog # A00081-3. Tested in IHC-P, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-APP/C99 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00081-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence of human APP/C99 (DAEFRHDSGYEVHHQKLVFFAEDVGSNK).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00081-3 is reactive to APP in Human

Applications

A00081-3 is guaranteed for IHC-P, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For APP (Source: Uniprot.Org, NCBI)

Uniprot ID

P05067

Gene ID

351

Gene Name

APP

Full Name

Amyloid-beta precursor protein

Weight

86.943kDa

Superfamily

APP family

Alternative Names

Abeta; Amyloid beta; beta-Amyloid Peptide APP AAA, ABETA, ABPP, AD1I, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4, APP amyloid beta precursor protein amyloid-beta precursor protein|alzheimer disease amyloid protein|amyloid beta (A4) precursor protein|amyloid beta A4 protein|amyloid precursor protein|beta-amyloid peptide|beta-amyloid peptide(1-40)|beta-amyloid peptide(1-42)|beta-amyloid precursor protein|cerebral vascular amyloid peptide|peptidase nexin-II|protease nexin-II|testicular tissue protein Li 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on APP, check out the APP Infographic

APP infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for APP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00081-3 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Kuang X, Zhou HJ, Thorne AH, Chen XN, Li LJ, Du JR. Front Aging Neurosci. 2017 Nov 2;9:353. doi: 10.3389/fnagi.2017.00353. eCollection 2017. Neuroprotective Effect of Ligustilide through Induction of α-Secretase Processing of Both APP and Klotho i...

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16 Customer Q&As for Anti-APP/C99 Antibody Picoband™

Question

We have tried in the past anti-APP/C99 antibody for IHC-P on eye pancreas in the past. I am using human, and We intend to use the antibody for WB next. We are interested in examining eye pancreas as well as brain cortex in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Z. Patel

Verified customer

Asked: 2020-04-30

Answer

I have checked the website and datasheets of our anti-APP/C99 antibody and it appears that A00081-3 has been validated on human in both IHC-P and WB. Thus A00081-3 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-04-30

Question

We have seen staining in human cerebrospinal fluid. Do you have any suggestions? Is anti-APP/C99 antibody supposed to stain cerebrospinal fluid positively?

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

According to literature cerebrospinal fluid does express APP. According to Uniprot.org, APP is expressed in frontal cortex, brain, leukocyte, cerebellum hippocampus, eye pancreas, liver, fibroblast, platelet, brain cortex, cerebrospinal fluid, blood vessel, fetal brain, plasma, among other tissues. Regarding which tissues have APP expression, here are a few articles citing expression in various tissues:
Blood vessel, Pubmed ID: 8248178
Brain, Pubmed ID: 2569763, 2881207, 2893289, 3035574, 3810169, 9338779
Brain cortex, Pubmed ID: 2893379, 3312495
Cerebrospinal fluid, Pubmed ID: 8229004, 22576872
Fetal brain, Pubmed ID: 2949367
Leukocyte, Pubmed ID: 1587857
Liver, Pubmed ID: 24275569
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 12665801

Boster Scientific Support

Answered: 2020-04-16

Question

Would A00081-3 anti-APP/C99 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

As indicated on the product datasheet, A00081-3 anti-APP/C99 antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-31

Question

Do you have a BSA free version of anti-APP/C99 antibody A00081-3 available?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-APP/C99 antibody A00081-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-31

Question

We are currently using anti-APP/C99 antibody A00081-3 for human tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-10

Answer

The anti-APP/C99 antibody (A00081-3) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-10

Question

I see that the anti-APP/C99 antibody A00081-3 works with IHC-P, what is the protocol used to produce the result images on the product page?

F. Rodriguez

Verified customer

Asked: 2019-06-05

Answer

You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-05

Question

Is a blocking peptide available for product anti-APP/C99 antibody (A00081-3)?

Verified Customer

Verified customer

Asked: 2019-01-15

Answer

We do provide the blocking peptide for product anti-APP/C99 antibody (A00081-3). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-01-15

Question

My colleagues were content with the WB result of your anti-APP/C99 antibody. However we have been able to see positive staining in fetal brain cell membrane using this antibody. Is that expected? Could you tell me where is APP supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-08-02

Answer

From what I have seen in literature, fetal brain does express APP. Generally APP expresses in cell membrane. Regarding which tissues have APP expression, here are a few articles citing expression in various tissues:
Blood vessel, Pubmed ID: 8248178
Brain, Pubmed ID: 2569763, 2881207, 2893289, 3035574, 3810169, 9338779
Brain cortex, Pubmed ID: 2893379, 3312495
Cerebrospinal fluid, Pubmed ID: 8229004, 22576872
Fetal brain, Pubmed ID: 2949367
Leukocyte, Pubmed ID: 1587857
Liver, Pubmed ID: 24275569
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 12665801

Boster Scientific Support

Answered: 2018-08-02

Question

Here is the WB image, lot number and protocol we used for blood vessel using anti-APP/C99 antibody A00081-3. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-11-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-11-10

Question

I was wanting to use your anti-APP/C99 antibody for IHC-P for human blood vessel on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human blood vessel identification?

D. Baker

Verified customer

Asked: 2017-11-09

Answer

It shows on the product datasheet, A00081-3 anti-APP/C99 antibody has been validated for IHC-P, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood vessel in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-11-09

Question

Is this A00081-3 anti-APP/C99 antibody reactive to the isotypes of APP?

Verified Customer

Verified customer

Asked: 2017-10-19

Answer

The immunogen of A00081-3 anti-APP/C99 antibody is A synthetic peptide corresponding to a sequence of human APP/C99(DAEFRHDSGYEVHHQKLVFFAEDVGSNK). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-10-19

Question

Would anti-APP/C99 antibody A00081-3 work for IHC-P with blood vessel?

Verified Customer

Verified customer

Asked: 2017-09-06

Answer

According to the expression profile of blood vessel, APP is highly expressed in blood vessel. So, it is likely that anti-APP/C99 antibody A00081-3 will work for IHC-P with blood vessel.

Boster Scientific Support

Answered: 2017-09-06

Question

you antibody to test anti-APP/C99 antibody A00081-3 on human blood vessel for research purposes, then I may be interested in using anti-APP/C99 antibody A00081-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

E. Miller

Verified customer

Asked: 2017-09-06

Answer

The products we sell, including anti-APP/C99 antibody A00081-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-09-06

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood vessel using anti-APP/C99 antibody A00081-3. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-09-06

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-09-06

Question

I have a question about product A00081-3, anti-APP/C99 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-06-23

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00081-3 anti-APP/C99 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-06-23

Question

Our lab want to know about using your anti-APP/C99 antibody for positive regulation of glycolytic process studies. Has this antibody been tested with western blotting on renal cancer tissue? We would like to see some validation images before ordering.

L. Edwards

Verified customer

Asked: 2014-11-20

Answer

I appreciate your inquiry. This A00081-3 anti-APP/C99 antibody is validated on human glioma tissue, renal cancer tissue. It is guaranteed to work for IHC-P, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2014-11-20

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A00081-3
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