Anti-beta Amyloid/APP Antibody Picoband™

APP antibody

Boster Bio Anti-beta Amyloid/APP Antibody Picoband™ catalog # PB9091. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PB9091
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

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Product Name

Anti-beta Amyloid/APP Antibody Picoband™

View all APP Antibodies

SKU/Catalog Number

PB9091

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-beta Amyloid/APP Antibody Picoband™ catalog # PB9091. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-beta Amyloid/APP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9091)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human APP (672-713aaDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA), different from the related mouse and rat sequences by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9091 is reactive to APP in Human, Mouse, Rat

Applications

PB9091 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Background of APP

beta Amyloid, also called Abeta or Abeta, denotes peptides of 36–43 amino acidsthat are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. It is mapped to 19q13.12. Several potential activities have been discovered for beta Amyloid, including activation of kinase enzymes, functioning as atranscription factor, and anti-microbial activity (potentially associated with beta Amyloid's pro-inflammatoryactivity). Moreover, monomeric beta Amyloid is indicated to protect neurons by quenching metal-inducible oxygen radical generation and thereby inhibiting neurotoxicity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For APP (Source: Uniprot.Org, NCBI)

Uniprot ID

P05067

Gene ID

351

Gene Name

APP

Full Name

Amyloid-beta precursor protein

Weight

86.943kDa

Superfamily

APP family

Alternative Names

Abeta; Amyloid beta; beta-Amyloid Peptide APP AAA, ABETA, ABPP, AD1I, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4, APP amyloid beta precursor protein amyloid-beta precursor protein|alzheimer disease amyloid protein|amyloid beta (A4) precursor protein|amyloid beta A4 protein|amyloid precursor protein|beta-amyloid peptide|beta-amyloid peptide(1-40)|beta-amyloid peptide(1-42)|beta-amyloid precursor protein|cerebral vascular amyloid peptide|peptidase nexin-II|protease nexin-II|testicular tissue protein Li 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on APP, check out the APP Infographic

APP infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for APP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9091 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Combined administration of D -galactose and aluminium induces Alzheimerlike lesions in brain

Zhang L,Zhang J,Gong Y,Lv L.Systematic and experimental investigations of the anti-colorectal cancer mediated by genistein. Biofactors.2020 Nov;46(6):974-982.doi:10.1002/biof.1677.Epub 2020 Sep 20.PMID:32951326.
Species: Human,Mouse
PB9091 usage in article: APP:IF, SAMPLE:CRC TISSUE, DILUTION: 1:100

Ou Qiao,Xinyu Zhang,Yi Zhang et al.Cerebralcare Granule® enhances memantine hydrochloride efficacy in APP/PS1 mice by ameliorating amyloid pathology and cognitive functions,08 April 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-366097/v1]
Species: Mouse
PB9091 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:NA

Gong YS, Hou FL, Guo J, Lin L, Zhu FY. Effects of Alcohol Intake on Cognitive Function and β-amyloid Protein in APP/PS1 Transgenic Mice. Food Chem Toxicol. 2021 Mar 15:112105. doi: 10.1016/j.fct.2021.112105. Epub ahead of print. PMID: 33737111.
Species: Mouse
PB9091 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:NA

Adapentpronitrile, a New Dipeptidyl Peptidase-IV Inhibitor, Ameliorates Diabetic Neuronal Injury Through Inhibiting Mitochondria-Related Oxidative Stress and Apoptosis

Kuang X, Zhou HJ, Thorne AH, Chen XN, Li LJ, Du JR. Front Aging Neurosci. 2017 Nov 2;9:353. doi: 10.3389/fnagi.2017.00353. eCollection 2017. Neuroprotective Effect of Ligustilide through Induction of α-Secretase Processing of Both APP and Klotho i...

Qin Ra, Yao Xx, Huang Zy. J Tradit Chin Med. 2012 Mar;32(1):63-6. Effects Of Compound Danshen Tablets On Spatial Cognition And Expression Of Brain Beta-Amyloid Precursor Protein In A Rat Model Of Alzheimer'S Disease.

Sun L, Zhou Xl, Yi Hp, Jiang Sj, Yuan H. Biotech Histochem. 2014 Oct;89(7):513-7. Doi: 10.3109/10520295.2014.904926. Epub 2014 May 7. Lead-Induced Morphological Changes And Amyloid Precursor Protein Accumulation In Adult Rat Hippocampus.

Li Wz, Li Wp, Huang Dk, Kan Hw, Wang X, Wu Wy, Yin Yy, Yao Yy. Behav Brain Res. 2012 Feb 1;227(1):142-9. Doi: 10.1016/J.Bbr.2011.10.038. Epub 2011 Oct 29. Dexamethasone And A????????-?????? Accelerate Learning And Memory Impairments Due To Elevate...

Nie J, Luo Y, Huang Xn, Gong Qh, Wu Q, Shi Js. Eur J Pharmacol. 2010 Jan 25;626(2-3):213-8. Doi: 10.1016/J.Ejphar.2009.09.039. Epub 2009 Sep 24. Icariin Inhibits Beta-Amyloid Peptide Segment 25-35 Induced Expression Of Beta-Secretase In Rat Hippoc...

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17 Customer Q&As for Anti-beta Amyloid/APP Antibody Picoband™

Question

We are currently using anti-beta Amyloid/APP antibody PB9091 for mouse tissue, and we are happy with the IHC-F results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-06

Answer

The anti-beta Amyloid/APP antibody (PB9091) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-06

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for plasma using anti-beta Amyloid/APP antibody PB9091. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-01-02

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-02

Question

My question regarding product PB9091, anti-beta Amyloid/APP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-17

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9091 anti-beta Amyloid/APP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-17

Question

I am looking for to test anti-beta Amyloid/APP antibody PB9091 on mouse plasma for research purposes, then I may be interested in using anti-beta Amyloid/APP antibody PB9091 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-20

Answer

The products we sell, including anti-beta Amyloid/APP antibody PB9091, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-20

Question

Our lab want to know about using your anti-beta Amyloid/APP antibody for positive regulation of 1-phosphatidylinositol-3-kinase activity studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

Thank you for your inquiry. This PB9091 anti-beta Amyloid/APP antibody is tested on human glioma tissue, hela whole cell lysates, a549 whole cell lysates, u2os whole cell lysates, rat brain tissue, mouse brain, renal cancer tissue, tonsil tissue. It is guaranteed to work for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-08

Question

We were content with the WB result of your anti-beta Amyloid/APP antibody. However we have seen positive staining in liver cell membrane using this antibody. Is that expected? Could you tell me where is APP supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-07

Answer

Based on literature, liver does express APP. Generally APP expresses in cell membrane. Regarding which tissues have APP expression, here are a few articles citing expression in various tissues:
Blood vessel, Pubmed ID: 8248178
Brain, Pubmed ID: 2569763, 2881207, 2893289, 3035574, 3810169, 9338779
Brain cortex, Pubmed ID: 2893379, 3312495
Cerebrospinal fluid, Pubmed ID: 8229004, 22576872
Fetal brain, Pubmed ID: 2949367
Leukocyte, Pubmed ID: 1587857
Liver, Pubmed ID: 24275569
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 12665801

Boster Scientific Support

Answered: 2019-10-07

Question

I see that the anti-beta Amyloid/APP antibody PB9091 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-01

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-01

Question

Does PB9091 detect the beta fragments, whole APP protein, or both?

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

PB9091 detects the beta fragments.

Boster Scientific Support

Answered: 2019-07-03

Question

Is there a BSA free version of anti-beta Amyloid/APP antibody PB9091 available?

Verified Customer

Verified customer

Asked: 2019-04-11

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-beta Amyloid/APP antibody PB9091 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-04-11

Question

Is this PB9091 anti-beta Amyloid/APP antibody reactive to the isotypes of APP?

Verified Customer

Verified customer

Asked: 2018-06-07

Answer

The immunogen of PB9091 anti-beta Amyloid/APP antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human APP (672-713aaDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA), different from the related mouse and rat sequences by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-06-07

Question

Would PB9091 anti-beta Amyloid/APP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

O. Li

Verified customer

Asked: 2018-04-26

Answer

It shows on the product datasheet, PB9091 anti-beta Amyloid/APP antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-04-26

Question

Our lab used your anti-beta Amyloid/APP antibody for IHC-P on brain in a previous experiment. I am using mouse, and We intend to use the antibody for IHC-F next. My question regards examining brain as well as blood vessel in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-F?

D. Johnson

Verified customer

Asked: 2018-01-19

Answer

I looked at the website and datasheets of our anti-beta Amyloid/APP antibody and I see that PB9091 has been validated on mouse in both IHC-P and IHC-F. Thus PB9091 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-F in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-F detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-01-19

Question

Does anti-beta Amyloid/APP antibody PB9091 work for IF with plasma?

A. Singh

Verified customer

Asked: 2017-08-24

Answer

According to the expression profile of plasma, APP is highly expressed in plasma. So, it is likely that anti-beta Amyloid/APP antibody PB9091 will work for IF with plasma.

Boster Scientific Support

Answered: 2017-08-24

Question

I was wanting to use your anti-beta Amyloid/APP antibody for IF for mouse plasma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse plasma identification?

B. Yang

Verified customer

Asked: 2015-06-01

Answer

As indicated on the product datasheet, PB9091 anti-beta Amyloid/APP antibody has been tested for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse plasma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-06-01

Question

We have observed staining in rat fetal brain. Do you have any suggestions? Is anti-beta Amyloid/APP antibody supposed to stain fetal brain positively?

R. Taylor

Verified customer

Asked: 2013-11-22

Answer

From what I have seen in literature fetal brain does express APP. From what I have seen in Uniprot.org, APP is expressed in frontal cortex, brain, leukocyte, cerebellum hippocampus, eye pancreas, liver, fibroblast, platelet, brain cortex, cerebrospinal fluid, blood vessel, fetal brain, plasma, among other tissues. Regarding which tissues have APP expression, here are a few articles citing expression in various tissues:
Blood vessel, Pubmed ID: 8248178
Brain, Pubmed ID: 2569763, 2881207, 2893289, 3035574, 3810169, 9338779
Brain cortex, Pubmed ID: 2893379, 3312495
Cerebrospinal fluid, Pubmed ID: 8229004, 22576872
Fetal brain, Pubmed ID: 2949367
Leukocyte, Pubmed ID: 1587857
Liver, Pubmed ID: 24275569
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 12665801

Boster Scientific Support

Answered: 2013-11-22

Question

Is a blocking peptide available for product anti-beta Amyloid/APP antibody (PB9091)?

J. Krishna

Verified customer

Asked: 2013-08-22

Answer

We do provide the blocking peptide for product anti-beta Amyloid/APP antibody (PB9091). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-08-22

Question

See attached the WB image, lot number and protocol we used for plasma using anti-beta Amyloid/APP antibody PB9091. Please let me know if you require anything else.

A. Gonzalez

Verified customer

Asked: 2013-04-01

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-04-01

Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:PB9091

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PB9091
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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