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Product Info Summary
| SKU: | M00081 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Amyloid beta A4 APP Rabbit Monoclonal Antibody
SKU/Catalog Number
M00081
BM4128 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Amyloid beta A4 APP Rabbit Monoclonal Antibody catalog # M00081. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Amyloid beta A4 APP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00081)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BGC-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Amyloid beta A4
Reactive Species
M00081 is reactive to APP in Human, Mouse, Rat
Observed Molecular Weight
105 kDa
Calculated molecular weight
86.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00081 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
IHC: human brain tissue
ICC/IF: SH-SY5Y cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature.
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Western blot analysis of Amyloid beta A4 expression in mouse brain lysate.
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All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human brain, using Amyloid beta A4 Antibody .
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Immunofluorescent analysis of SH-SY5Y cells, using Amyloid beta A4 Antibody .
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Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution)
Specific Publications For Anti-Amyloid beta A4 APP Rabbit Monoclonal Antibody (M00081)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Amyloid beta A4 APP Rabbit Monoclonal Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cerebellum hippocampus using anti-Amyloid beta A4 Rabbit Monoclonal antibody M00081. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-23
Question
We bought anti-Amyloid beta A4 Rabbit Monoclonal antibody for WB on cerebrospinal fluid in a previous project. I am using rat, and We intend to use the antibody for IP next. Our lab want to know about examining cerebrospinal fluid as well as fibroblast in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
I looked at the website and datasheets of our anti-Amyloid beta A4 Rabbit Monoclonal antibody and I see that M00081 has been validated on rat in both WB and IP. Thus M00081 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-12
Question
I see that the anti-Amyloid beta A4 Rabbit Monoclonal antibody M00081 works with IF, what is the protocol used to produce the result images on the product page?
N. Zhao
Verified customer
Asked: 2019-07-29
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-29
Question
See below the WB image, lot number and protocol we used for cerebellum hippocampus using anti-Amyloid beta A4 Rabbit Monoclonal antibody M00081. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-17
Question
Is a blocking peptide available for product anti-Amyloid beta A4 Rabbit Monoclonal antibody (M00081)?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
We do provide the blocking peptide for product anti-Amyloid beta A4 Rabbit Monoclonal antibody (M00081). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-26
Question
We are currently using anti-Amyloid beta A4 Rabbit Monoclonal antibody M00081 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-10
Answer
The anti-Amyloid beta A4 Rabbit Monoclonal antibody (M00081) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-10
Question
My lab would like using your anti-Amyloid beta A4 Rabbit Monoclonal antibody for negative regulation of mitochondrion organization studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
P. Jackson
Verified customer
Asked: 2019-03-20
Answer
Thanks for your inquiry. This M00081 anti-Amyloid beta A4 Rabbit Monoclonal antibody is validated on mouse brain. It is guaranteed to work for IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-03-20
Question
Does anti-Amyloid beta A4 Rabbit Monoclonal antibody M00081 work for IF with cerebellum hippocampus?
Verified Customer
Verified customer
Asked: 2018-12-06
Answer
According to the expression profile of cerebellum hippocampus, APP is highly expressed in cerebellum hippocampus. So, it is likely that anti-Amyloid beta A4 Rabbit Monoclonal antibody M00081 will work for IF with cerebellum hippocampus.
Boster Scientific Support
Answered: 2018-12-06
Question
We have seen staining in mouse blood vessel. What should we do? Is anti-Amyloid beta A4 Rabbit Monoclonal antibody supposed to stain blood vessel positively?
Verified Customer
Verified customer
Asked: 2018-03-15
Answer
From literature blood vessel does express APP. From Uniprot.org, APP is expressed in frontal cortex, brain, leukocyte, cerebellum hippocampus, eye pancreas, liver, fibroblast, platelet, brain cortex, cerebrospinal fluid, blood vessel, fetal brain, plasma, among other tissues. Regarding which tissues have APP expression, here are a few articles citing expression in various tissues:
Blood vessel, Pubmed ID: 8248178
Brain, Pubmed ID: 2569763, 2881207, 2893289, 3035574, 3810169, 9338779
Brain cortex, Pubmed ID: 2893379, 3312495
Cerebrospinal fluid, Pubmed ID: 8229004, 22576872
Fetal brain, Pubmed ID: 2949367
Leukocyte, Pubmed ID: 1587857
Liver, Pubmed ID: 24275569
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 12665801
Boster Scientific Support
Answered: 2018-03-15
Question
Is this M00081 anti-Amyloid beta A4 Rabbit Monoclonal antibody reactive to the isotypes of APP?
Verified Customer
Verified customer
Asked: 2017-12-12
Answer
The immunogen of M00081 anti-Amyloid beta A4 Rabbit Monoclonal antibody is A synthesized peptide derived from human Amyloid beta A4 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-12
Question
Will M00081 anti-Amyloid beta A4 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-05
Answer
It shows on the product datasheet, M00081 anti-Amyloid beta A4 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-05
Question
My colleagues were content with the WB result of your anti-Amyloid beta A4 Rabbit Monoclonal antibody. However we have seen positive staining in eye pancreas cell membrane using this antibody. Is that expected? Could you tell me where is APP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-08-25
Answer
According to literature, eye pancreas does express APP. Generally APP expresses in cell membrane. Regarding which tissues have APP expression, here are a few articles citing expression in various tissues:
Blood vessel, Pubmed ID: 8248178
Brain, Pubmed ID: 2569763, 2881207, 2893289, 3035574, 3810169, 9338779
Brain cortex, Pubmed ID: 2893379, 3312495
Cerebrospinal fluid, Pubmed ID: 8229004, 22576872
Fetal brain, Pubmed ID: 2949367
Leukocyte, Pubmed ID: 1587857
Liver, Pubmed ID: 24275569
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 12665801
Boster Scientific Support
Answered: 2017-08-25
Question
I was wanting to use your anti-Amyloid beta A4 Rabbit Monoclonal antibody for IF for mouse cerebellum hippocampus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cerebellum hippocampus identification?
Verified Customer
Verified customer
Asked: 2017-08-22
Answer
It shows on the product datasheet, M00081 anti-Amyloid beta A4 Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cerebellum hippocampus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-22
Question
We are interested in to test anti-Amyloid beta A4 Rabbit Monoclonal antibody M00081 on mouse cerebellum hippocampus for research purposes, then I may be interested in using anti-Amyloid beta A4 Rabbit Monoclonal antibody M00081 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Edwards
Verified customer
Asked: 2017-05-16
Answer
The products we sell, including anti-Amyloid beta A4 Rabbit Monoclonal antibody M00081, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-05-16
Question
My question regarding product M00081, anti-Amyloid beta A4 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Zhao
Verified customer
Asked: 2017-01-10
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00081 anti-Amyloid beta A4 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-01-10
Question
Is there a BSA free version of anti-Amyloid beta A4 Rabbit Monoclonal antibody M00081 available?
B. Krishna
Verified customer
Asked: 2016-02-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Amyloid beta A4 Rabbit Monoclonal antibody M00081 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-02-11
