Anti-ATP5G2 Antibody

ATP5MC2 antibody

Boster Bio Anti-ATP5G2 Antibody (Catalog # A11264). Tested in IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: A11264
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: IHC

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Product Name

Anti-ATP5G2 Antibody

View all ATP5MC2 Antibodies

SKU/Catalog Number

A11264

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-ATP5G2 Antibody (Catalog # A11264). Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ATP5G2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11264)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human ATP5G2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A11264 is reactive to ATP5MC2 in Human

Applications

A11264 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

14.637kDa

Background of ATP5MC2

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element. HAMAP-Rule MF_01396

Dyer M.R., Biochem. J. 293:51-64(1993).
Higuti T., Biochim. Biophys. Acta 1173:87-90(1993).
Farrell L.B., Biochem. Biophys. Res. Commun. 144:1257-1264(1987).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For ATP5MC2 (Source: Uniprot.org, NCBI)

Gene Name

ATP5MC2

Full Name

ATP synthase F(0) complex subunit C2, mitochondrial

Weight

14.637kDa

Superfamily

ATPase C chain family

Alternative Names

ATP synthase F(0) complex subunit C2, mitochondrial ATP5MC2 ATP5A, ATP5G2 ATP synthase membrane subunit c locus 2 ATP synthase F(0) complex subunit C2, mitochondrial|ATP synthase c subunit|ATP synthase lipid-binding protein, mitochondrial|ATP synthase proteolipid P2|ATP synthase proton-transporting mitochondrial F(0) complex subunit C2|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C2 (subunit 9)|ATP synthase, H+ transporting, mitochondrial Fo complex subunit C2 (subunit 9)|ATPase protein 9|ATPase subunit C|dicyclohexylcarbodiimide (DCCD)-reactive proteolipid subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ATP5MC2, check out the ATP5MC2 Infographic

ATP5MC2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ATP5MC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-ATP5G2 Antibody

Question

Would A11264 anti-ATP5G2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

It shows on the product datasheet, A11264 anti-ATP5G2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-29

Question

Is this A11264 anti-ATP5G2 antibody reactive to the isotypes of Atp5g2?

Verified Customer

Verified customer

Asked: 2020-04-17

Answer

The immunogen of A11264 anti-ATP5G2 antibody is Synthesized peptide derived from internal of human ATP5G2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-17

Question

I am interested in to test anti-ATP5G2 antibody A11264 on human liver for research purposes, then I may be interested in using anti-ATP5G2 antibody A11264 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

The products we sell, including anti-ATP5G2 antibody A11264, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-09

Question

See attached the WB image, lot number and protocol we used for liver using anti-ATP5G2 antibody A11264. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-06

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-06

Question

I see that the anti-ATP5G2 antibody A11264 works with IHC, what is the protocol used to produce the result images on the product page?

O. Anderson

Verified customer

Asked: 2019-06-17

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-17

Question

We are currently using anti-ATP5G2 antibody A11264 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2018-06-04

Answer

The anti-ATP5G2 antibody (A11264) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-06-04

Order DetailsPrice
A11264-100uL

100uL

$360

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A11264
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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