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Product Info Summary
| SKU: | A12221-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-ATP5G3 Antibody
SKU/Catalog Number
A12221-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ATP5G3 Antibody (Catalog# A12221-1). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATP5G3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12221-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ATP5G3. Immunogen sequence location: 1-50
Reactive Species
A12221-1 is reactive to ATP5G3 in Human, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
14.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12221-1 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Recommend Dilution
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:40000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of A549 cells, using ATP5G3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using ATP5G3 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-ATP5G3 Antibody (A12221-1)
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4 Customer Q&As for Anti-ATP5G3 Antibody
Question
Can you help my question with product A12221-1, anti-ATP5G3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12221-1 anti-ATP5G3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-03
Question
Is this A12221-1 anti-ATP5G3 antibody reactive to the isotypes of ATP5G3?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The immunogen of A12221-1 anti-ATP5G3 antibody is A peptide derived from human ATP5G3. Immunogen sequence location: 1-50. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-24
Question
Is a blocking peptide available for product anti-ATP5G3 antibody (A12221-1)?
Verified Customer
Verified customer
Asked: 2019-02-01
Answer
We do provide the blocking peptide for product anti-ATP5G3 antibody (A12221-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-02-01
Question
We are currently using anti-ATP5G3 antibody A12221-1 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-20
Answer
The anti-ATP5G3 antibody (A12221-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-20
