Click image to see more details
Product Info Summary
| SKU: | A12221-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC |
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Product info
Product Name
Anti-ATP5G3 Antibody
SKU/Catalog Number
A12221-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ATP5G3 Antibody (Catalog# A12221-1). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATP5G3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12221-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ATP5G3. Immunogen sequence location: 1-50
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12221-1 is reactive to ATP5MC3 in Human, Rat
Applications
A12221-1 is guaranteed for ELISA, IF, IHC-P, ICC Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
14.693kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using ATP5G3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of A549 cells, using ATP5G3 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For ATP5MC3 (Source: Uniprot.org, NCBI)
Gene Name
ATP5MC3
Full Name
ATP synthase F(0) complex subunit C3, mitochondrial
Weight
14.693kDa
Superfamily
ATPase C chain family
Alternative Names
ATP synthase F(0) complex subunit C3, mitochondrial ATP5MC3 ATP5G3, P3 ATP synthase membrane subunit c locus 3 ATP synthase F(0) complex subunit C3, mitochondrial|ATP synthase lipid-binding protein, mitochondrial|ATP synthase proteolipid P3|ATP synthase proton-transporting mitochondrial F(0) complex subunit C3|ATP synthase subunit 9|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C3 (subunit 9)|ATP synthase, H+ transporting, mitochondrial Fo complex subunit C3 (subunit 9)|ATP synthase, mitochondrial, C subunit-3|ATPase protein 9|ATPase subunit C|dicyclohexylcarbodiimide (DCCD)-reactive proteolipid subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP5MC3, check out the ATP5MC3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP5MC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATP5G3 Antibody (A12221-1)
Hello CJ!
No publications found for A12221-1
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-ATP5G3 Antibody
Question
Can you help my question with product A12221-1, anti-ATP5G3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12221-1 anti-ATP5G3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-03
Question
Is this A12221-1 anti-ATP5G3 antibody reactive to the isotypes of ATP5G3?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The immunogen of A12221-1 anti-ATP5G3 antibody is A peptide derived from human ATP5G3. Immunogen sequence location: 1-50. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-24
Question
Is a blocking peptide available for product anti-ATP5G3 antibody (A12221-1)?
Verified Customer
Verified customer
Asked: 2019-02-01
Answer
We do provide the blocking peptide for product anti-ATP5G3 antibody (A12221-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-02-01
Question
We are currently using anti-ATP5G3 antibody A12221-1 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-20
Answer
The anti-ATP5G3 antibody (A12221-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-20
