Anti-BIN1/Amphiphysin Ii Monoclonal Antibody

Product Name

Anti-BIN1/Amphiphysin Ii Monoclonal Antibody

View all BIN1 Antibodies

SKU/Catalog Number

M01551

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-BIN1/Amphiphysin Ii Monoclonal Antibody (Catalog # M01551). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-BIN1/Amphiphysin Ii Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01551)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 99D IgG2b

Isotype

IgG2b

Immunogen

Anti-BIN1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with a fragment portion of recombinant human BIN1 protein followed by hybridoma development.

Reactive Species

M01551 is reactive to BIN1 in Human, Mouse

Calculated molecular weight

64.7 kDa

Background of BIN1

Bin1 is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, actin organization, programmed cell death, stress responses, and transcriptional control. The first mammalian BAR protein to be discovered, Amphiphysin I (AmphI), was identified in an immunoscreen for proteins associated with the plasma membranes of synaptic neurons, functions in the control of clathrin-dependent synaptic vesicle endocytosis. The mammalian Bin1 gene was first identified in a two hybrid screen for polypeptides that bind to the N-terminal Myc box 1 (MB1) portion of the c-Myc oncoprotein. Bin1 is similar to AmphI in overall structure, with an N-terminal BAR domain and a C-terminal SH3 domain. However, the Bin1 gene is more complex than the AmphI gene, encoding at least seven different splice variants that differ widely in subcellular localization, tissue distribution, and ascribed functions. Alternate splicing of the Bin1 gene results in ten transcript variants encoding different isoform. Bin1 is expressed ubiquitously in mammalian cells. Certain splice variants of Bin1 are expressed in the neurons, muscle cells or tumor cells. Bin1 may act as a cancer suppressor and inhibits malignant cell transformation. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Bin1 has also been implicated in Alzheimer disease and cardiac disease. Defects in Bin1 are the cause of centronuclear myopathy autosomal recessive; also known as autosomal recessive myotubular myopathy.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01551 is guaranteed for ELISA, Flow Cytometry, IP, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5000-1:50000
Flow Cytometry: 0.5-1x10^6 cells
IHC: 1:100-1:500
IP: 10-100 µL
WB: 1:500-1:1500
Anti-BIN1 antibody has been tested for use in ELISA, IP, and Western Blot. This antibody is suitable for use in IHC and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.

Validation Images & Assay Conditions

See full target information BIN1

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