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Product Info Summary
| SKU: | M00381 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-TPM3 Antibody
SKU/Catalog Number
M00381
Size
50 µl
Description
Boster Bio Anti-TPM3 Antibody (Catalog # M00381). Tested in WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TPM3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00381)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1649CT306.29.13
Isotype
IgG1,k
Immunogen
This TPM3 antibody is generated from a mouse immunized with a recombinant protein of human TPM3.
Reactive Species
M00381 is reactive to TPM3 in Human, Mouse
Calculated molecular weight
33.0 kDa
Background of TPM3
Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00381 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-TPM3 Antibody at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: NIH/3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 33 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-TPM3 Antibody (M00381)
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