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ACTA1 antibody (RP1070) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 48 Publications

rp1070 actin primary antibodies ihc testing 3

IHC analysis of Actin using anti-Actin antibody (RP1070).
Actin was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Actin Antibody (RP1070) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-Actin/ACTA1 Antibody Picoband®

ACTA1 antibody

48 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IHC, WB

Tested Applications

ACTA1 antibody was positively detected by WB in:

human placenta tissue, human Hela whole cell, human HepG2 whole cell, human 293T whole cell, human A431 whole cell, human U87 whole cell, human U937 whole cell, human K562 whole cell, rat heart tissue, rat brain tissue, rat kidney tissue, mouse heart tissue, mouse brain tissue, mouse spleen tissue, mouse kidney tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

ACTA1 antibody was positively detected by IHC in:

human breast cancer tissue, human colorectal adenocarcinoma tissue, human placenta tissue, human tonsil tissue, mouse heart tissue, rat heart tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Rabbit

Predicted

Bovine

Predicted

Sheep

Predicted

Monkey

Predicted

Zebrafish

Predicted

Product Information

Product Name

Anti-Actin/ACTA1 Antibody Picoband®
View all ACTA1 Antibodies

Form

Lyophilized

Uniprot

P68133

Gene ID

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Actin, identical to the related mouse and rat sequences.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

42 kDa

Calculated Molecular Weight

42.1 kDa

Background of ACTA1

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Cite This Product

Anti-Actin/ACTA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1070)

Protocols

Western Blot protocol for ACTA1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for ACTA1 antibody

Review Protocol

Publications

Pan-cancer analysis of UGGT1 in human tumors and experimental validation in breast cancer

Published: 2025-07-24

Journal: Scientific Reports

Application: WB

Impact Factor: 3.90

Species: Human

PMID: 40707677

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Anti-Actin/ACTA1 Antibody Picoband®

ACTA1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Pan-cancer analysis of UGGT1 in human tumors and experimental validation in breast cancer

Secoisolariciresinol diglucoside Ameliorates Osteoarthritis via Nuclear factor-erythroid 2-related factor-2/ nuclear factor kappa B Pathway: In vitro and in vivo experiments

Dl-3-n-butylphthalide prevents chronic restraint stress-induced depression-like behaviors and cognitive impairment via regulating CaMKII/CREB/BDNF signaling pathway in hippocampus

Bioinformatic and biochemical studies of formononetin against liver injure

Immunohistochemical evidence of striated muscle cells within midfacial superficial musculoaponeurotic system

Identification of Filamin A Mechanobinding Partner II: Fimbacin Is a Novel Actin Cross-Linking and Filamin A Binding Protein

A pectic polysaccharide from water decoction of Xinjiang Lycium barbarum fruit protects against intestinal endoplasmic reticulum stress

Retinoic Acid-Induced Protein 14 (RAI14) Promotes mTOR-Mediated Inflammation Under Inflammatory Stress and Chemical Hypoxia in a U87 Glioblastoma Cell Line

miR-340 alleviates chemoresistance of osteosarcoma cells by targeting ZEB1

Myoendothelial gap junctions mediate regulation of angiopoietin-2-induced vascular hyporeactivity after hypoxia through connexin 43-gated cAMP transfer

Clinical biocharacterization of immunophenotype in hepatocellular carcinoma patients

Tumor grade related expression of neuroglobin is negatively regulated by PPARγ and confers antioxidant activity in glioma progression

Down-regulation of microRNA-338-3p promoted angiogenesis in hepatocellular carcinoma

Potential neuroprotection of protodioscin against cerebral ischemia-reperfusion injury in rats through intervening inflammation and apoptosis

miR-494 inhibits ovarian cancer cell proliferation and promotes apoptosis by targeting FGFR2

Downregulation of SUMF2 gene in ovalbumin-induced rat model of allergic inflammation

The expansion of autologous adipose-derived stem cells in vitro for the functional reconstruction of nasal mucosal tissue

miR-153 inhibits epithelial-to-mesenchymal transition in hepatocellular carcinoma by targeting Snail

Tailoring of the titanium surface by preparing cardiovascular endothelial extracellular matrix layer on the hyaluronic acid micro-pattern for improving biocompatibility

The up-regulation of endothelin-1 and down-regulation of miRNA-125a-5p, -155, and -199a/b-3p in human atherosclerotic coronary artery

The Effect of 5'-Adenylic Acid on Hepatic Proteome of Mice Radiated by 60Co γ-ray

Neurotransmitter noradrenaline downregulate cytoskeletal protein expression of VSMCs

Targeting of the Sonic Hedgehog Pathway by Atractylenolides Promotes Chondrogenic Differentiation of Mesenchymal Stem Cells

Engineered myocardial tissues constructed in vivo using cardiomyocyte-like cells derived from bone marrow mesenchymal stem cells in rats

Constitutive heat shock protein 70 interacts with α-enolase and protects cardiomyocytes against oxidative stress

Involvement of calmodulin and actin in directed differentiation of rat cortical neural stem cells into neurons

Isoproterenol-induced FKBP12.6/12 downregulation is modulated by ETA and ETB receptors and reversed by argirhein, a derivative of rhein

High dietary intake of medium-chain fatty acids during pregnancy in rats prevents later-life obesity in their offspring

In Vitro and In Vivo Inhibition of MRP Gene Expression and Reversal of Multidrug Resistance by siRNA

Oriented immobilization of anti-CD34 antibody on titanium surface for self-endothelialization induction

Proteomics Analysis of Differential Expression of Chicken Brain Tissue Proteins in Response to the Neurovirulent H5N1 Avian Influenza Virus Infection

Calcium-sensing receptor activation contributed to apoptosis stimulates TRPC6 channel in rat neonatal ventricular myocytes

Biocompatibility of pure titanium modified by human endothelial cell-derived extracellular matrix

CPU228, a derivative of dofetilide, relieves cardiac dysfunction by normalizing FKBP12.6, NADPH oxidase and protein kinase C ε in the myocardium

Autologous bone marrow mesenchymal cell transplantation improves left ventricular function in a rabbit model of dilated cardiomyopathy

Homocysteine-induced enhanced expression of tissue factor in human vascular smooth muscle cells

Endothelin receptor antagonist CPU0213 and vitamin E reverse downregulation of FKBP12.6 and SERCA2a: A role of hyperphosphorylation of PKCε

CPU86017 and its isomers improve hypoxic pulmonary hypertension by attenuating increased ETA receptor expression and extracellular matrix accumulation

Over-expressed and truncated midkines promote proliferation of BGC823 cells in vitro and tumor growth in vivo

Effect of Dopamine Receptor 1 on Apoptosis of Cultured Neonatal Rat Cardiomyocytes in Simulated Ischaemia/Reperfusion

Genistein induces G 2 /M cell cycle arrest via stable activation of ERK1/2 pathway in MDA-MB-231 breast cancer cells

A novel synthetic oleanolic acid derivative (CPU-II2) attenuates liver fibrosis in mice through regulating the function of hepatic stellate cells

siRNA targeting midkine inhibits gastric cancer cells growth and induces apoptosis involved caspase-3,8,9 activation and mitochondrial depolarization

A cytotoxin isolated from Agkistrodon acutus snake venom induces apoptosis via Fas pathway in A549 cells

Calcium-sensing receptor induces rat neonatal ventricular cardiomyocyte apoptosis

Inhibition of the PI3K pathway sensitizes fludarabine-induced apoptosis in human leukemic cells through an inactivation of MAPK-dependent pathway

Antitumor effects of human ribonuclease inhibitor gene transfected on B16 melanoma cells

Down-regulation of apoptosis-inducing factor protein by RNA interference inhibits UVA-induced cell death

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