Anti-Actin/ACTA1 Antibody

Boster Bio Anti-Actin/ACTA1 Antibody catalog # RP1070. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 65 publication(s).

Product Info Summary

SKU: RP1070
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Actin/ACTA1 Antibody

See all ACTA1 products

SKU/Catalog Number

RP1070

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Actin/ACTA1 Antibody catalog # RP1070. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Actin/ACTA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1070)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Actin (359-377aa ITKQEYDEAGPSIVHRKCF), identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1070 is reactive to ACTA1 in Human, Mouse, Rat

Applications

RP1070 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For ACTA1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P68133

Gene ID

58

Gene Name

ACTA1

Full Name

Actin, alpha skeletal muscle

Weight

42.051kDa

Superfamily

actin family

Alternative Names

ACTA; Actin; actin, alpha 1, skeletal muscle; alpha skeletal muscle actin; alpha skeletal muscle; alpha-actin-1; ASMA; CFTD; CFTDM; MPFD; NEM1; NEM2; NEM3 ACTA1 ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3, SHPM actin alpha 1, skeletal muscle actin, alpha skeletal muscle|nemaline myopathy type 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ACTA1, check out the ACTA1 Infographic

ACTA1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ACTA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

RP1070 has been cited in 65 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Cheng J,Luan J,Chen P,Kuang X,Jiang P,Zhang R,Chen S,Cheng F,Gou X.Immunosuppressive receptor LILRB1 acts as a potential regulator in hepatocellular carcinoma by integrating with SHP1.Cancer Biomark.2020; 28(3):309-319.doi:10.3233/CBM-190940.PMID:32390601
Species: Human
RP1070 usage in article: APP:WB, SAMPLE:LO2,MHCC97L,HUH 7 AND HEPG2 CELLS, DILUTION:NA

Sandulescu T, Weniger J, Philippou S, Mücke T, Naumova EA, Arnold WH. Immunohistochemical evidence of striated muscle cells within midfacial superficial musculoaponeurotic system. Ann Anat. 2020 Nov 19:151647. doi: 10.1016/j.aanat.2020.151647. Epub ahead
Species: Human

Jia-Shen decoction-medicated serum inhibits angiotensin-II induced cardiac fibroblast proliferation via the TGF-?1/Smad signaling pathway

Expression changes of ionic channels in early phase of cultured rat atrial myocytes induced by rapid pacing

The Effect of 5?-Adenylic Acid on Hepatic Proteome of Mice Radiated by 60Co ?-ray

Protective effects of non-mitogenic human acidic fibroblast growth factor on hydrogen peroxide-induced damage to cardiomyocytes in vitro

Inhibition of human La protein by RNA interference downregulates hepatitis B virus mRNA in 2.2.15 cells

Engineered myocardial tissues constructed in vivo using cardiomyocyte-like cells derived from bone marrow mesenchymal stem cells in rats

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Chen Y, Liu W, Li W, Gao C. Exp Mol Pathol. 2010 Apr;88(2):311-5. Doi: 10.1016/J.Yexmp.2009.12.002. Epub 2009 Dec 11. Autologous Bone Marrow Mesenchymal Cell Transplantation Improves Left Ventricular Function In A Rabbit Model Of Dilated Cardiomyo...

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Lu X, Cheng C, Zhu S, Yang Y, Zheng L, Wang G, Shu X, Wu K, Liu K, Tong Q. Oncol Rep. 2010 Oct;24(4):981-7. Satb1 Is An Independent Prognostic Marker For Gastric Cancer In A Chinese Population.

Wang Yh, Wu Mw, Yang Ak, Zhang Wd, Sun J, Liu Tr, Chen Yf. Med Oncol. 2011 Dec;28 Suppl 1:S360-6. Doi: 10.1007/S12032-010-9737-3. Epub 2010 Nov 11. Cox-2 Gene Increases Tongue Cancer Cell Proliferation And Invasion Through Vegf-C Pathway.

Liao L, Zheng R, Wang C, Gao J, Ying Y, Ning Q, Luo X. Pediatr Res. 2011 Jun;69(6):497-503. Doi: 10.1203/Pdr.0B013E31821769Bd. The Influence Of Down-Regulation Of Suppressor Of Cellular Signaling Proteins By Rnai On Glucose Transport Of Intrauteri...

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Yang L, Ye D, Ye W, Jiao C, Zhu L, Mao J, Jacob Tj, Wang L, Chen L. J Cell Physiol. 2011 Oct;226(10):2516-26. Doi: 10.1002/Jcp.22596. Clc-3 Is A Main Component Of Background Chloride Channels Activated Under Isotonic Conditions By Autocrine Atp In...

Xie S, Xie N, Li Y, Wang P, Zhang C, Li Q, Liu X, Deng J, Zhang C, Lv C. Mol Cell Biochem. 2012 Feb;361(1-2):305-14. Doi: 10.1007/S11010-011-1116-7. Epub 2011 Oct 20. Upregulation Of Trb2 Induced By Mir-98 In The Early Lesions Of Large Artery Of T...

Zhang Y, Yao W, Qiu J, Qian W, Zhu C, Zhang C. Neurosci Lett. 2011 Nov 14;505(2):71-5. Doi: 10.1016/J.Neulet.2011.09.055. Epub 2011 Oct 1. The Involvement Of Down-Regulation Of Cdh1-Apc In Hippocampal Neuronal Apoptosis After Global Cerebral Ische...

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Hu C, Zou Mj, Zhao L, Lu N, Sun Yj, Gou Sh, Xi T, Guo Ql. Toxicol Lett. 2012 Apr 5;210(1):78-86. Doi: 10.1016/J.Toxlet.2012.01.019. Epub 2012 Jan 31. E Platinum, A Newly Synthesized Platinum Compound, Induces Autophagy Via Inhibiting Phosphorylati...

Shi Jh, Hu Dh, Zhang Zf, Bai Xz, Wang Ht, Zhu Xx, Su Yj, Tang Cw. Arch Dermatol Res. 2012 Apr;304(3):209-15. Doi: 10.1007/S00403-012-1204-X. Epub 2012 Jan 12. Reduced Expression Of Microtubule-Associated Protein 1 Light Chain 3 In Hypertrophic Scars.

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Leng X, Wang X, Pang W, Zhan R, Zhang Z, Wang L, Gao X, Qian L. Cell Stress Chaperones. 2013 Jul;18(4):483-93. Doi: 10.1007/S12192-013-0404-4. Epub 2013 Jan 19. Evidence Of A Role For Both Anti-Hsp70 Antibody And Endothelial Surface Membrane Hsp70...

Deng X, Zhong Y, Gu L, Shen W, Guo J. Brain Res Bull. 2013 May;94:56-62. Doi: 10.1016/J.Brainresbull.2013.02.007. Epub 2013 Mar 7. Mir-21 Involve In Erk-Mediated Upregulation Of Mmp9 In The Rat Hippocampus Following Cerebral Ischemia.

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Zhu X, Wu L, Qiao H, Han T, Chen S, Liu X, Jiang R, Wei Y, Feng D, Zhang Y, Ma Y, Zhang S, Zhang J. J Cell Biochem. 2013 Dec;114(12):2643-53. Doi: 10.1002/Jcb.24611. Autophagy Stimulates Apoptosis In Her2-Overexpressing Breast Cancers Treated By L...

Ma Yx, Guo Z, Sun T. Neurosci Lett. 2013 Aug 9;549:130-4. Doi: 10.1016/J.Neulet.2013.05.028. Epub 2013 May 25. Cgrp Inhibits Norepinephrine Induced Apoptosis With Restoration Of Bcl-2/Bax In Cultured Cardiomyocytes Of Rat.

Tao L, Fu R, Wang X, Yao J, Zhou Y, Dai Q, Li Z, Lu N, Wang W. Toxicol Lett. 2014 Jul 3;228(1):1-12. Doi: 10.1016/J.Toxlet.2014.04.002. Epub 2014 Apr 19. Ll-202, A Newly Synthesized Flavonoid, Inhibits Tumor Growth Via Inducing G(2)/M Phase Arrest...

Li Xf, Chen Dp, Ouyang Fz, Chen Mm, Wu Y, Kuang Dm, Zheng L. J Hepatol. 2015 Jan;62(1):131-9. Doi: 10.1016/J.Jhep.2014.08.023. Epub 2014 Aug 23. Increased Autophagy Sustains The Survival And Pro-Tumourigenic Effects Of Neutrophils In Human Hepatoc...

Zhou D, Wang Y, Tokunaga K, Huang F, Sun B, Yang R. Virus Res. 2015 Jan 2;195:25-34. Doi: 10.1016/J.Virusres.2014.08.021. Epub 2014 Sep 6. The Hiv-1 Accessory Protein Vpr Induces The Degradation Of The Anti-Hiv-1 Agent Apobec3G Through A Vprbp-Med...

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18 Customer Q&As for Anti-Actin/ACTA1 Antibody

Question

I have a question about product RP1070, anti-Actin/ACTA1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-03-24

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1070 anti-Actin/ACTA1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-24

Question

We are interested in to test anti-Actin/ACTA1 antibody RP1070 on mouse skeletal muscle for research purposes, then I may be interested in using anti-Actin/ACTA1 antibody RP1070 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-21

Answer

The products we sell, including anti-Actin/ACTA1 antibody RP1070, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-21

Question

Is this RP1070 anti-Actin/ACTA1 antibody reactive to the isotypes of ACTA1?

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

The immunogen of RP1070 anti-Actin/ACTA1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Actin (359-377aa ITKQEYDEAGPSIVHRKCF), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-13

Question

Is a blocking peptide available for product anti-Actin/ACTA1 antibody (RP1070)?

Verified Customer

Verified customer

Asked: 2020-01-27

Answer

We do provide the blocking peptide for product anti-Actin/ACTA1 antibody (RP1070). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-27

Question

We were happy with the WB result of your anti-Actin/ACTA1 antibody. However we have been able to see positive staining in biceps brachii cytoskeleton. using this antibody. Is that expected? Could you tell me where is ACTA1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

According to literature, biceps brachii does express ACTA1. Generally ACTA1 expresses in cytoplasm, cytoskeleton. Regarding which tissues have ACTA1 expression, here are a few articles citing expression in various tissues:
Skeletal muscle, Pubmed ID: 6190133, 15489334

Boster Scientific Support

Answered: 2019-12-23

Question

Do you have a BSA free version of anti-Actin/ACTA1 antibody RP1070 available?

Verified Customer

Verified customer

Asked: 2019-11-20

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Actin/ACTA1 antibody RP1070 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-11-20

Question

I see that the anti-Actin/ACTA1 antibody RP1070 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-20

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-20

Question

See below the WB image, lot number and protocol we used for skeletal muscle using anti-Actin/ACTA1 antibody RP1070. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-03

Question

Does RP1070 anti-Actin/ACTA1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

T. Krishna

Verified customer

Asked: 2019-09-02

Answer

You can see on the product datasheet, RP1070 anti-Actin/ACTA1 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-02

Question

We are currently using anti-Actin/ACTA1 antibody RP1070 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-04-17

Answer

The anti-Actin/ACTA1 antibody (RP1070) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-04-17

Question

Our lab used your anti-Actin/ACTA1 antibody for IHC on biceps brachii in a previous project. I am using rat, and We want to use the antibody for WB next. We are interested in examining biceps brachii as well as skeletal muscle in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-01-02

Answer

I viewed the website and datasheets of our anti-Actin/ACTA1 antibody and I see that RP1070 has been validated on rat in both IHC and WB. Thus RP1070 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-01-02

Question

We are currently using anti-Actin/ACTA1 antibody RP1070 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?

C. Thomas

Verified customer

Asked: 2018-12-31

Answer

The anti-Actin/ACTA1 antibody (RP1070) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-12-31

Question

I was wanting to use your anti-Actin/ACTA1 antibody for IHC for mouse skeletal muscle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse skeletal muscle identification?

Verified Customer

Verified customer

Asked: 2018-09-28

Answer

As indicated on the product datasheet, RP1070 anti-Actin/ACTA1 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse skeletal muscle in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-09-28

Question

We have observed staining in human biceps brachii. What should we do? Is anti-Actin/ACTA1 antibody supposed to stain biceps brachii positively?

Verified Customer

Verified customer

Asked: 2017-06-15

Answer

Based on literature biceps brachii does express ACTA1. Based on Uniprot.org, ACTA1 is expressed in biceps brachii, skeletal muscle, among other tissues. Regarding which tissues have ACTA1 expression, here are a few articles citing expression in various tissues:
Skeletal muscle, Pubmed ID: 6190133, 15489334

Boster Scientific Support

Answered: 2017-06-15

Question

I am looking for using your anti-Actin/ACTA1 antibody for mesenchyme migration studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

H. Lewis

Verified customer

Asked: 2017-05-04

Answer

Thank you for your inquiry. This RP1070 anti-Actin/ACTA1 antibody is validated on rat liver tissue, tissue lysate, kidney tissue, mouse spleen tissue, hela whole cell lysate, colo320 whole cell lysate, smmc whole cell lysate, a549 whole cell lysate, intestine tissue, mammary cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-05-04

Question

Will anti-Actin/ACTA1 antibody RP1070 work on primate WB with skeletal muscle?

J. Baker

Verified customer

Asked: 2016-01-18

Answer

Our lab technicians have not validated anti-Actin/ACTA1 antibody RP1070 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Actin/ACTA1 antibody RP1070 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate skeletal muscle in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-01-18

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skeletal muscle using anti-Actin/ACTA1 antibody RP1070. Let me know if you need anything else.

R. Williams

Verified customer

Asked: 2014-04-02

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-04-02

Question

Does anti-Actin/ACTA1 antibody RP1070 work for IHC with skeletal muscle?

O. Carter

Verified customer

Asked: 2013-01-03

Answer

According to the expression profile of skeletal muscle, ACTA1 is highly expressed in skeletal muscle. So, it is likely that anti-Actin/ACTA1 antibody RP1070 will work for IHC with skeletal muscle.

Boster Scientific Support

Answered: 2013-01-03

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