Anti-Actin/ACTA1 Antibody Picoband™ (monoclonal, 3H5)

ACTA1 antibody

Boster Bio Anti-Actin/ACTA1 Antibody Picoband™ (monoclonal, 3H5) catalog # M02014-5. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 9 publication(s).

Product Info Summary

SKU: M02014-5
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: IHC, WB

Product Name

Anti-Actin/ACTA1 Antibody Picoband™ (monoclonal, 3H5)

View all ACTA1 Antibodies

SKU/Catalog Number

M02014-5

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Actin/ACTA1 Antibody Picoband™ (monoclonal, 3H5) catalog # M02014-5. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Actin/ACTA1 Antibody Picoband™ (monoclonal, 3H5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02014-5)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Monoclonal

Clone Number

3H5

Isotype

Mouse IgG1

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Actin, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02014-5 is reactive to ACTA1 in Human, Mouse, Rat

Applications

M02014-5 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

43 kDa

Calculated molecular weight

42.051kDa

Background of ACTA1

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ACTA1 (Source: Uniprot.org, NCBI)

Gene Name

ACTA1

Full Name

Actin, alpha skeletal muscle

Weight

42.051kDa

Superfamily

actin family

Alternative Names

ACTA; Actin; actin, alpha 1, skeletal muscle; alpha skeletal muscle actin; alpha skeletal muscle; alpha-actin-1; ASMA; CFTD; CFTDM; MPFD; NEM1; NEM2; NEM3 ACTA1 ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3, SHPM actin alpha 1, skeletal muscle actin, alpha skeletal muscle|nemaline myopathy type 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACTA1, check out the ACTA1 Infographic

ACTA1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACTA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M02014-5 has been cited in 9 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Grape Seed Proanthocyanidins (GSPs) Inhibit the Development of Cutaneous Squamous Cell Carcinoma by Regulating the hsa_circ_0070934/miR-136-5p/PRAF2 Axis

MDR1/P-gp and VEGF Synergistically Enhance the Invasion of Hep-2 Cells with Multidrug Resistance Induced by Taxol

Telomerase catalyzed fluorescent probes for sensitive protein profiling based on one-dimensional microfluidic beads array

Liao L,Huang L,Wei X,Yin L,Wei X,Li T.Bioinformatic and biochemical studies of formononetin against liver injure.Life Sci.2021 Feb 16:119229.doi:10.1016/j.lfs.2021.119229.Epub ahead of print.PMID:33607154.
Species: Mouse
M02014-5 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA

Liao L,Huang L,Wei X,Yin L,Wei X,Li T.Bioinformatic and biochemical studies of formononetin against liver injure.Life Sci.2021 Feb 16:119229.doi:10.1016/j.lfs.2021.119229.Epub ahead of print.PMID:33607154.
Species: Mouse
M02014-5 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA

Cheng J,Luan J,Chen P,Kuang X,Jiang P,Zhang R,Chen S,Cheng F,Gou X.Immunosuppressive receptor LILRB1 acts as a potential regulator in hepatocellular carcinoma by integrating with SHP1.Cancer Biomark.2020; 28(3):309-319.doi:10.3233/CBM-190940.PMID:32390601
Species: Human
M02014-5 usage in article: APP:WB, SAMPLE:LO2,MHCC97L,HUH 7 AND HEPG2 CELLS, DILUTION:NA

Xu T,Pan L,Li L,Hu S,Zhou H,Yang C,Yang J,Li H,Liu Y,Meng X,Li J.MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.doi: 10.1111/jc
Species: Human
M02014-5 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1000

Wang R, Wu G, Dai T, Lang Y, Chi Z, Yang S, Dong D. Naringin attenuates renal interstitial fibrosis by regulating the TGF-β/Smad signaling pathway and inflammation. Exp Ther Med. 2021 Jan;21(1):66. doi: 10.3892/etm.2020.9498. Epub 2020 Nov 23. PMID: 33365
Species: Rat
M02014-5 usage in article: APP:IF, SAMPLE:KIDNEY TISSUE, DILUTION:1:70

Liao J, Wu Q, Qian C, Zhao N, Zhao Z, Lu K, Zhang S, Dong Q, Chen L, Li Q, Du Y. TRPV4 blockade suppresses atrial fibrillation in sterile pericarditis rats. JCI Insight. 2020 Oct 29:137528. doi: 10.1172/jci.insight.137528. Epub ahead of print. PMID: 33119
Species: Rat
M02014-5 usage in article: APP:IHC, SAMPLA:ATRIA TISSUE, DILUTION:1:50

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Total: $370

SKU:M02014-5

In stock, 1 left.

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M02014-5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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