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Product Info Summary
| SKU: | M02014-5 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Actin/ACTA1 Antibody Picoband® (monoclonal, 3H5)
SKU/Catalog Number
M02014-5
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Actin/ACTA1 Antibody Picoband® (monoclonal, 3H5) catalog # M02014-5. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Actin/ACTA1 Antibody Picoband® (monoclonal, 3H5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02014-5)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Monoclonal
Clone Number
3H5
Isotype
Mouse IgG1
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Actin, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M02014-5 is reactive to ACTA1 in Human, Mouse, Rat
Observed Molecular Weight
43 kDa
Calculated molecular weight
42.1 kDa
Background of ACTA1
Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02014-5 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Positive Control
WB: human HepG2 whole cell, human SK-OV-3 whole cell, human PANC-1 whole cell, human Hela whole cell, human A549 whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell, mouse HEPA1-6 whole cell
IHC: human bladder cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Actin/ACTA1 using anti-Actin/ACTA1 antibody (M02014-5).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human SK-OV-3 whole cell lysates,
Lane 3: human PANC-1 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Actin/ACTA1 antigen affinity purified monoclonal antibody (Catalog # M02014-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Actin/ACTA1 at approximately 43 kDa. The expected band size for Actin/ACTA1 is at 43 kDa.
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IHC analysis of Actin/ACTA1 using anti-Actin/ACTA1 antibody (M02014-5).
Actin/ACTA1 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml mouse anti-Actin/ACTA1 Antibody (M02014-5) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Specific Publications For Anti-Actin/ACTA1 Antibody Picoband® (monoclonal, 3H5) (M02014-5)
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