Anti-CD146 Rabbit Monoclonal Antibody, Clone#RM249

Boster Bio Anti-CD146 Rabbit Monoclonal Antibody, Clone#RM249 catalog # M01683-2. Tested in IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M01683-2
Size: 100ul
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-CD146 Rabbit Monoclonal Antibody, Clone#RM249

See all MCAM/CD146 products

SKU/Catalog Number

M01683-2

Size

100ul

Description

Boster Bio Anti-CD146 Rabbit Monoclonal Antibody, Clone#RM249 catalog # M01683-2. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD146 Rabbit Monoclonal Antibody, Clone#RM249 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01683-2)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM249

Immunogen

A synthetic peptide corresponding to the residues near C-terminus of human CD146

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01683-2 is reactive to MCAM in Human

Applications

M01683-2 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (IHC): 1:200-1:500 dilution;
Western Blot (WB): 1:1000-1:2000 dilution.

Validation Images & Assay Conditions

Gene/Protein Information For MCAM (Source: Uniprot.Org, NCBI)

Uniprot ID

P43121

Gene ID

4162

Gene Name

MCAM

Full Name

Cell surface glycoprotein MUC18

Weight

71.607kDa

Alternative Names

CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin MCAM CD146, HEMCAM, METCAM, MUC18, MelCAM melanoma cell adhesion molecule cell surface glycoprotein MUC18|Gicerin|S-endo 1 endothelial-associated antigen|cell surface glycoprotein P1H12|melanoma adhesion molecule|melanoma-associated antigen A32|melanoma-associated antigen MUC18

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MCAM, check out the MCAM Infographic

MCAM infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MCAM: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for M01683-2

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5 Customer Q&As for Anti-CD146 Rabbit Monoclonal Antibody, Clone#RM249

Question

Our lab want to know about using your anti-CD146 Rabbit Monoclonal antibody, Clone#RM249 for anatomical structure morphogenesis studies. Has this antibody been tested with western blotting on breast cancer tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-02-06

Answer

I appreciate your inquiry. This M01683-2 anti-CD146 Rabbit Monoclonal antibody, Clone#RM249 is tested on a375 cells, breast cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-02-06

Question

We ordered your anti-CD146 Rabbit Monoclonal antibody, Clone

Verified Customer

Verified customer

Asked: 2019-12-18

Answer

I viewed the website and datasheets of our anti-CD146 Rabbit Monoclonal antibody, Clone

Boster Scientific Support

Answered: 2019-12-18

Question

We are currently using anti-CD146 Rabbit Monoclonal antibody, Clone#RM249 M01683-2 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-29

Answer

The anti-CD146 Rabbit Monoclonal antibody, Clone#RM249 (M01683-2) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-29

Question

We have observed staining in human melanoma. Do you have any suggestions? Is anti-CD146 Rabbit Monoclonal antibody, Clone#RM249 supposed to stain melanoma positively?

T. Mangal

Verified customer

Asked: 2016-11-09

Answer

From what I have seen in literature melanoma does express MCAM. From what I have seen in Uniprot.org, MCAM is expressed in popliteal artery, melanoma, trachea, aortic endothelium, pns, cervix carcinoma, liver, among other tissues. Regarding which tissues have MCAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Liver, Pubmed ID: 19159218
Melanoma, Pubmed ID: 2602381, 8378324
PNS, Pubmed ID: 15489334
Trachea, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2016-11-09

Question

My boss were well pleased with the WB result of your anti-CD146 Rabbit Monoclonal antibody, Clone

D. Williams

Verified customer

Asked: 2016-07-01

Answer

From literature, melanoma does express MCAM. Generally MCAM expresses in membrane. Regarding which tissues have MCAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Liver, Pubmed ID: 19159218
Melanoma, Pubmed ID: 2602381, 8378324
PNS, Pubmed ID: 15489334
Trachea, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2016-07-01

$382

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
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M01683-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$382.00

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