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Product Info Summary
| SKU: | M01683-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD146 Rabbit Monoclonal Antibody, Clone#RM249
SKU/Catalog Number
M01683-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CD146 Rabbit Monoclonal Antibody, Clone#RM249 (Catalog # M01683-2). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD146 Rabbit Monoclonal Antibody, Clone#RM249 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01683-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM249
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to the residues near C-terminus of human CD146
Cross-reactivity
This antibody reacts to human CD146 (Cell surface glycoprotein MUC18). This antibody may also react to mouse or rat CD146, as predicted by immunogen homology
Reactive Species
M01683-2 is reactive to MCAM in Human
Calculated molecular weight
71.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01683-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:200-1:500 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of A375 cell lysate, using Anti-CD146 RM249 at a 1:1000 dilution, showed CD146 (~110 kDa) expression in A375 cells.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-CD146 RM249 at a 1:400 dilution.
Specific Publications For Anti-CD146 Rabbit Monoclonal Antibody, Clone#RM249 (M01683-2)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-CD146 Rabbit Monoclonal Antibody, Clone#RM249
Question
Our lab want to know about using your anti-CD146 Rabbit Monoclonal antibody, Clone#RM249 for anatomical structure morphogenesis studies. Has this antibody been tested with western blotting on breast cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
I appreciate your inquiry. This M01683-2 anti-CD146 Rabbit Monoclonal antibody, Clone#RM249 is tested on a375 cells, breast cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-06
Question
We ordered your anti-CD146 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
I viewed the website and datasheets of our anti-CD146 Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-12-18
Question
We are currently using anti-CD146 Rabbit Monoclonal antibody, Clone#RM249 M01683-2 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
The anti-CD146 Rabbit Monoclonal antibody, Clone#RM249 (M01683-2) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-29
Question
We have observed staining in human melanoma. Do you have any suggestions? Is anti-CD146 Rabbit Monoclonal antibody, Clone#RM249 supposed to stain melanoma positively?
T. Mangal
Verified customer
Asked: 2016-11-09
Answer
From what I have seen in literature melanoma does express MCAM. From what I have seen in Uniprot.org, MCAM is expressed in popliteal artery, melanoma, trachea, aortic endothelium, pns, cervix carcinoma, liver, among other tissues. Regarding which tissues have MCAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Liver, Pubmed ID: 19159218
Melanoma, Pubmed ID: 2602381, 8378324
PNS, Pubmed ID: 15489334
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2016-11-09
Question
My boss were well pleased with the WB result of your anti-CD146 Rabbit Monoclonal antibody, Clone
D. Williams
Verified customer
Asked: 2016-07-01
Answer
From literature, melanoma does express MCAM. Generally MCAM expresses in membrane. Regarding which tissues have MCAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Liver, Pubmed ID: 19159218
Melanoma, Pubmed ID: 2602381, 8378324
PNS, Pubmed ID: 15489334
Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2016-07-01
