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Product Info Summary
| SKU: | A00470-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA (Cap) |
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Product info
Product Name
Anti-Galectin 1 Lgals1 Antibody
SKU/Catalog Number
A00470-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Galectin 1 Lgals1 Antibody catalog # A00470-2. Tested in ELISA applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Galectin 1 Lgals1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00470-2)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Galectin 1 recombinant protein (Position: A2-E135).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00470-2 is reactive to Lgals1 in Mouse
Calculated molecular weight
14.9 kDa
Background of Lgals1
Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. It is thought to play a role in creating immune tolerance in pregnancy. It has been found that Galectin-1-mediated production of IL6 may assist in augmenting the innate immune response against NiV. Galectin-1 may also be a significant factor that augments the efficiency of the HIV-1 infection process.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00470-2 is guaranteed for ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA Capture (Matched Antibody Pair), 2-8μg/ml
Validation Images & Assay Conditions
Click image to see more details
Sandwich ELISA - Recombinant mouse Galectin 1 protein standard curve.
Use in combination with reagents from Mouse Galectin 1 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0763).
Specific Publications For Anti-Galectin 1 Lgals1 Antibody (A00470-2)
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Galectin 1 Lgals1 Antibody
Question
Is there a BSA free version of anti-Galectin 1 antibody A00470-2 available?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Galectin 1 antibody A00470-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-17
Question
My question regarding product A00470-2, anti-Galectin 1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00470-2 anti-Galectin 1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-25
Question
I see that the anti-Galectin 1 antibody A00470-2 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-15
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammary cancer using anti-Galectin 1 antibody A00470-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-12
Question
We are currently using anti-Galectin 1 antibody A00470-2 for mouse tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
The anti-Galectin 1 antibody (A00470-2) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-04
Question
I was wanting to use your anti-Galectin 1 antibody for ELISA for mouse mammary cancer on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse mammary cancer identification?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
You can see on the product datasheet, A00470-2 anti-Galectin 1 antibody has been tested for ELISA on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse mammary cancer in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-03
Question
Does anti-Galectin 1 antibody A00470-2 work for ELISA with mammary cancer?
K. Baker
Verified customer
Asked: 2019-05-27
Answer
According to the expression profile of mammary cancer, LGALS1 is highly expressed in mammary cancer. So, it is likely that anti-Galectin 1 antibody A00470-2 will work for ELISA with mammary cancer.
Boster Scientific Support
Answered: 2019-05-27
Question
We were happy with the WB result of your anti-Galectin 1 antibody. However we have been able to see positive staining in brain extracellular using this antibody. Is that expected? Could you tell me where is LGALS1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-04-10
Answer
From literature, brain does express LGALS1. Generally LGALS1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have LGALS1 expression, here are a few articles citing expression in various tissues:
Hepatoma, Pubmed ID: 2719646
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 2719964
Lung fibroblast, Pubmed ID: 12761501
Lymphocyte, Pubmed ID: 1988031
Mammary cancer, Pubmed ID: 19497882
Melanoma, Pubmed ID: 1386213
Placenta, Pubmed ID: 3065332, 3611046, 10369126, 18824694
Placenta, and Promyelocytic leukemia, Pubmed ID: 2910856
Placenta, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Spleen, Pubmed ID: 2383549
Thalamus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-04-10
Question
We have seen staining in mouse brain. Are there any suggestions? Is anti-Galectin 1 antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-01-31
Answer
From literature brain does express LGALS1. From Uniprot.org, LGALS1 is expressed in adipose tissue, lung, placenta promyelocytic leukemia, hepatoma, placenta, lymphocyte, lung fibroblast, thalamus, placenta skin, brain, platelet, brain, cajal-retzius cell fetal brain cortex, melanoma, spleen, mammary cancer, liver, among other tissues. Regarding which tissues have LGALS1 expression, here are a few articles citing expression in various tissues:
Hepatoma, Pubmed ID: 2719646
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 2719964
Lung fibroblast, Pubmed ID: 12761501
Lymphocyte, Pubmed ID: 1988031
Mammary cancer, Pubmed ID: 19497882
Melanoma, Pubmed ID: 1386213
Placenta, Pubmed ID: 3065332, 3611046, 10369126, 18824694
Placenta, and Promyelocytic leukemia, Pubmed ID: 2910856
Placenta, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Spleen, Pubmed ID: 2383549
Thalamus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-01-31
Question
See attached the WB image, lot number and protocol we used for mammary cancer using anti-Galectin 1 antibody A00470-2. Please let me know if you require anything else.
M. Miller
Verified customer
Asked: 2019-01-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-28
Question
Is this A00470-2 anti-Galectin 1 antibody reactive to the isotypes of LGALS1?
S. Wu
Verified customer
Asked: 2018-12-06
Answer
The immunogen of A00470-2 anti-Galectin 1 antibody is E. coli-derived mouse Galectin 1 recombinant protein (Position: A2-E135). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-06
Question
We are interested in to test anti-Galectin 1 antibody A00470-2 on mouse mammary cancer for research purposes, then I may be interested in using anti-Galectin 1 antibody A00470-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Baker
Verified customer
Asked: 2017-01-11
Answer
The products we sell, including anti-Galectin 1 antibody A00470-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-01-11
Question
Is a blocking peptide available for product anti-Galectin 1 antibody (A00470-2)?
S. Moore
Verified customer
Asked: 2016-03-01
Answer
We do provide the blocking peptide for product anti-Galectin 1 antibody (A00470-2). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2016-03-01
Question
Would A00470-2 anti-Galectin 1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Gonzalez
Verified customer
Asked: 2014-11-11
Answer
As indicated on the product datasheet, A00470-2 anti-Galectin 1 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-11-11
