Anti-Galectin 1 Lgals1 Antibody Picoband™

Galectin-1 antibody

Boster Bio Anti-Galectin 1 Lgals1 Antibody Picoband™ catalog # A00470-2. Tested in ELISA applications. This antibody reacts with Mouse.

Product Info Summary

SKU: A00470-2
Size: 100 μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: ELISA

Product Name

Anti-Galectin 1 Lgals1 Antibody Picoband™

View all Galectin-1 Antibodies

SKU/Catalog Number

A00470-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Galectin 1 Lgals1 Antibody Picoband™ catalog # A00470-2. Tested in ELISA applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Galectin 1 Lgals1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00470-2)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse Galectin 1 recombinant protein (Position: A2-E135).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00470-2 is reactive to LGALS1 in Mouse

Applications

A00470-2 is guaranteed for ELISA Boster Guarantee

Observed Molecular Weight

15 kDa

Calculated molecular weight

14.716kDa

Background of Galectin-1

Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. It is thought to play a role in creating immune tolerance in pregnancy. It has been found that Galectin-1-mediated production of IL6 may assist in augmenting the innate immune response against NiV. Galectin-1 may also be a significant factor that augments the efficiency of the HIV-1 infection process.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA Capture (Matched Antibody Pair), 2-8μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For LGALS1 (Source: Uniprot.org, NCBI)

Gene Name

LGALS1

Full Name

Galectin-1

Weight

14.716kDa

Alternative Names

14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; BHL; DKFZp686E23103; GAL1; gal-1; Galaptin; galectin 1; Galectin1; Galectin-1; GBP; HBL; HLBP14; HPL; L-14; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; LGALS1; Putative MAPK-activating protein PM12; S-Lac lectin 1 LGALS1 GAL1, GBP galectin 1 galectin-1|14 kDa laminin-binding protein|14 kDa lectin|HBL|HLBP14|HPL|S-Lac lectin 1|beta-galactoside-binding lectin L-14-I|beta-galactoside-binding protein 14kDa|epididymis secretory sperm binding protein|gal-1|galaptin|lactose-binding lectin 1|lectin, galactoside-binding, soluble, 1|putative MAPK-activating protein PM12

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LGALS1, check out the LGALS1 Infographic

LGALS1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LGALS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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14 Customer Q&As for Anti-Galectin 1 Lgals1 Antibody Picoband™

Question

Is there a BSA free version of anti-Galectin 1 antibody A00470-2 available?

Verified Customer

Verified customer

Asked: 2020-03-17

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Galectin 1 antibody A00470-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-17

Question

My question regarding product A00470-2, anti-Galectin 1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00470-2 anti-Galectin 1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-25

Question

I see that the anti-Galectin 1 antibody A00470-2 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-15

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-15

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammary cancer using anti-Galectin 1 antibody A00470-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-09-12

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-12

Question

We are currently using anti-Galectin 1 antibody A00470-2 for mouse tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-04

Answer

The anti-Galectin 1 antibody (A00470-2) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-04

Question

I was wanting to use your anti-Galectin 1 antibody for ELISA for mouse mammary cancer on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse mammary cancer identification?

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

You can see on the product datasheet, A00470-2 anti-Galectin 1 antibody has been tested for ELISA on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse mammary cancer in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-03

Question

Does anti-Galectin 1 antibody A00470-2 work for ELISA with mammary cancer?

K. Baker

Verified customer

Asked: 2019-05-27

Answer

According to the expression profile of mammary cancer, LGALS1 is highly expressed in mammary cancer. So, it is likely that anti-Galectin 1 antibody A00470-2 will work for ELISA with mammary cancer.

Boster Scientific Support

Answered: 2019-05-27

Question

We were happy with the WB result of your anti-Galectin 1 antibody. However we have been able to see positive staining in brain extracellular using this antibody. Is that expected? Could you tell me where is LGALS1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-04-10

Answer

From literature, brain does express LGALS1. Generally LGALS1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have LGALS1 expression, here are a few articles citing expression in various tissues:
Hepatoma, Pubmed ID: 2719646
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 2719964
Lung fibroblast, Pubmed ID: 12761501
Lymphocyte, Pubmed ID: 1988031
Mammary cancer, Pubmed ID: 19497882
Melanoma, Pubmed ID: 1386213
Placenta, Pubmed ID: 3065332, 3611046, 10369126, 18824694
Placenta, and Promyelocytic leukemia, Pubmed ID: 2910856
Placenta, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Spleen, Pubmed ID: 2383549
Thalamus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-04-10

Question

We have seen staining in mouse brain. Are there any suggestions? Is anti-Galectin 1 antibody supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2019-01-31

Answer

From literature brain does express LGALS1. From Uniprot.org, LGALS1 is expressed in adipose tissue, lung, placenta promyelocytic leukemia, hepatoma, placenta, lymphocyte, lung fibroblast, thalamus, placenta skin, brain, platelet, brain, cajal-retzius cell fetal brain cortex, melanoma, spleen, mammary cancer, liver, among other tissues. Regarding which tissues have LGALS1 expression, here are a few articles citing expression in various tissues:
Hepatoma, Pubmed ID: 2719646
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 2719964
Lung fibroblast, Pubmed ID: 12761501
Lymphocyte, Pubmed ID: 1988031
Mammary cancer, Pubmed ID: 19497882
Melanoma, Pubmed ID: 1386213
Placenta, Pubmed ID: 3065332, 3611046, 10369126, 18824694
Placenta, and Promyelocytic leukemia, Pubmed ID: 2910856
Placenta, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Spleen, Pubmed ID: 2383549
Thalamus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-01-31

Question

See attached the WB image, lot number and protocol we used for mammary cancer using anti-Galectin 1 antibody A00470-2. Please let me know if you require anything else.

M. Miller

Verified customer

Asked: 2019-01-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-01-28

Question

Is this A00470-2 anti-Galectin 1 antibody reactive to the isotypes of LGALS1?

S. Wu

Verified customer

Asked: 2018-12-06

Answer

The immunogen of A00470-2 anti-Galectin 1 antibody is E. coli-derived mouse Galectin 1 recombinant protein (Position: A2-E135). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-12-06

Question

We are interested in to test anti-Galectin 1 antibody A00470-2 on mouse mammary cancer for research purposes, then I may be interested in using anti-Galectin 1 antibody A00470-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

S. Baker

Verified customer

Asked: 2017-01-11

Answer

The products we sell, including anti-Galectin 1 antibody A00470-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-01-11

Question

Is a blocking peptide available for product anti-Galectin 1 antibody (A00470-2)?

S. Moore

Verified customer

Asked: 2016-03-01

Answer

We do provide the blocking peptide for product anti-Galectin 1 antibody (A00470-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-03-01

Question

Would A00470-2 anti-Galectin 1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

D. Gonzalez

Verified customer

Asked: 2014-11-11

Answer

As indicated on the product datasheet, A00470-2 anti-Galectin 1 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-11-11

Order DetailsPrice
A00470-2

100μg

$370
A00470-2-10ug

10μg sample (liquid)

$99
A00470-2-Biotin

100 μg Biotin conjugated

$570
A00470-2-Cy3

100 μg Cy3 conjugated

$570
A00470-2-Dylight488

100 μg Dylight488 conjugated

$570
A00470-2-Dylight550

100 μg Dylight550 conjugated

$570
A00470-2-Dylight594

100 μg Dylight594 conjugated

$570
A00470-2-FITC

100 μg FITC conjugated

$570
A00470-2-HRP

100 μg HRP conjugated

$570
A00470-2-APC

100 μg APC conjugated

$670
A00470-2-PE

100 μg PE conjugated

$670
A00470-2-carrier-free

Carrier Free

$370

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