Rabbit IgG polyclonal antibody for Pleiotrophin(PTN) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Cited in 1 publication(s).
Anti-Pleiotrophin/PTN Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
Dystroglycan 1 (DAG1) is a laminin binding component of the dystrophin-glycoprotein complex which provides a connection between the subsarcolemmal cytoskeleton and the extracellular matrix. The N-terminal domain of alpha-dystroglycan is secreted into the cerebrospinal fluid. The effect of DAG1 on the nervous system remains vague. The complete dystroglycan complex is expressed in a various tissues and has a role in processes such as laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. DAG1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic decrease of DAG1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, making muscle fibers more susceptible to necrosis.
|Product Name||Anti-Pleiotrophin/PTN Antibody
See all Pleiotrophin/PTN primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for Pleiotrophin/PTN detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Pleiotrophin/PTN information: Molecular Weight: 18942 MW; Subcellular Localization: Secreted; Tissue Specificity: Osteoblast and brain.|
|Cite This Product||Anti-Pleiotrophin/PTN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1414)|
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|Anti-Pleiotrophin/PTN Antibody may replace the following items: sc 74443|sc 1394|sc 1395|sc 20716.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Pleiotrophin(150-168aa QAESKKKKKEGKKQEKMLD), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For PTN (Source: Uniprot.org, NCBI)
|NCBI Gene Id||5764|
|Species Of This Entry||Human|
|Alternative Names||Pleiotrophin/PTN|HARP; HBBM; HB-GAM; HBGF8; HBNF; HBNF1; HBNF-1; Heparin-binding brain mitogen; HNGF-8; NEGF1HBGF-8; neurite growth-promoting factor 1; neurite growth-promoting factor1); OSF-1; Osteoblast-specific factor 1; Pleiotrophin; PTN|
|Post Tranlational Modifications||Phosphorylation; Nitration; Cleavage; Methylation; Dephosphorylation; Nitrosylation; Deiodination; Citrullination; Ubiquitination; Myristoylation|
|Gene Location||7q33, on Chromasome 7, gene sequence: NC_000007.14 (137227341..137343733, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For PTN (Source: Uniprot.org, NCBI)
|Protein Function||Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells (PubMed:1768439, PubMed:1733956). Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation (PubMed:11278720). Binds to cell-surface target proteins via their chondroitin sulfate groups (PubMed:26896299). Down-regulates PTPRZ1 activity (PubMed:16814777).|
|Tissue Specificity||Osteoblast and brain.|
|Research Areas||Cell Cycle and Replication|
|Related Diseases||Neoplasms; Malignant Neoplasms; Tumor Angiogenesis; Malignant Paraganglionic Neoplasm; Nervousness; Pathologic Neovascularization; Neoplasm Metastasis; Carcinoma; Lymphoma; Glioblastoma; Anaplastic Lymphoma; Pain; Tissue Adhesions; Malignant Neoplasm Of Breast; Brain Neoplasms; Cell Invasion; Mammary Neoplasms; Melanoma; Fracture|
|Related Pathways||Angiogenesis; Regeneration; Cell Proliferation; Localization; Cell Growth; Cell Migration; Pathogenesis; Cell Adhesion; Reflex; Osteoblast Differentiation; Neurogenesis; Locomotion; Cell Cycle; Secretion; Endothelial Cell Migration; Chemotaxis; System Development; Rna Interference; Cell-cell Adhesion; Gene Silencing|
|Background||Pleiotrophin(PTN) also known as heparin-binding brain mitogen(HBBM) or heparin-binding growth factor 8(HBGF-8) or neurite growth-promoting factor 1(NEGF1) or heparin affinity regulatory peptide(HARP) or heparin binding growth associated molecule(HB-GAM) is a protein that in humans is encoded by the PTN gene. PTN is the first member of a family of developmentally regulated cytokines. The PTN gene is mapped to 7q33-q34. A mutant PTN that contained only the first N-terminal 40 amino acids was a dominant negative. Pleiotrophin is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. Pleiotrophin binds to cell-surface nucleolin as a low affinity receptor. This binding can inhibit HIV infection.|
|Scientific References||PMID: 1701634 by Tezuka K.I., et al. Isolation of mouse and human cDNA clones encoding a protein expressed specifically in osteoblasts and brain tissues.|
PMID: 2270483 by Li Y.-S., et al. Cloning and expression of a developmentally regulated protein that induces mitogenic and neurite outgrowth activity.
Our Boster Quality Guarantee for Anti-Pleiotrophin/PTN Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: HT1080 Cell Lysate
Lane 5: SMMC Cell Lysate
IHC(P): Human Mammary Cancer Tissue
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