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Product Info Summary
| SKU: | M00602-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CD68/Sr D1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00602-1
BM4593 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD68/Sr D1 Rabbit Monoclonal Antibody catalog # M00602-1. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD68/Sr D1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00602-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GBE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD68
Reactive Species
M00602-1 is reactive to CD68 in Human, Mouse, Rat
Observed Molecular Weight
37 kDa
Calculated molecular weight
37.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00602-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of CD68 expression in Jurkat cell lysate.
Specific Publications For Anti-CD68/Sr D1 Rabbit Monoclonal Antibody (M00602-1)
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Customer Q&As
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4 Customer Q&As for Anti-CD68/Sr D1 Rabbit Monoclonal Antibody
Question
We have seen staining in rat skin. Any tips? Is anti-CD68/Sr D1 Rabbit Monoclonal antibody supposed to stain skin positively?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
From literature skin does express CD68. From Uniprot.org, CD68 is expressed in leukocyte, spleen umbilical cord blood, adipose tissue, skin, platelet, liver, among other tissues. Regarding which tissues have CD68 expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 16625196
Liver, Pubmed ID: 19159218
Platelet, Pubmed ID: 16263699
Skin, Pubmed ID: 15489334
Spleen, and Umbilical cord blood, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-09-03
Question
We are currently using anti-CD68/Sr D1 Rabbit Monoclonal antibody M00602-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
The anti-CD68/Sr D1 Rabbit Monoclonal antibody (M00602-1) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-14
Question
I am interested in using your anti-CD68/Sr D1 Rabbit Monoclonal antibody for negative regulation of dendritic cell antigen processing and presentation studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-09-04
Answer
We appreciate your inquiry. This M00602-1 anti-CD68/Sr D1 Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-09-04
Question
My team were content with the WB result of your anti-CD68/Sr D1 Rabbit Monoclonal antibody. However we have seen positive staining in leukocyte isoform short: cell membrane using this antibody. Is that expected? Could you tell me where is CD68 supposed to be expressed?
B. Wu
Verified customer
Asked: 2017-07-11
Answer
Based on literature, leukocyte does express CD68. Generally CD68 expresses in isoform short: cell membrane. Regarding which tissues have CD68 expression, here are a few articles citing expression in various tissues:
Adipose tissue, Pubmed ID: 16625196
Liver, Pubmed ID: 19159218
Platelet, Pubmed ID: 16263699
Skin, Pubmed ID: 15489334
Spleen, and Umbilical cord blood, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-07-11
