Anti-Claudin-1 CLDN1 Antibody

Product Name

Anti-Claudin-1 CLDN1 Antibody

View all Claudin-1 Antibodies

SKU/Catalog Number

A01585-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Claudin-1 CLDN1 Antibody (Catalog # A01585-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Rat.

Storage & Handling

CLDN1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Claudin-1 CLDN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01585-1)

Host

Rabbit

Contents

CLDN1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

Anti-CLDN1 antibody was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of human CLDN1. The immunogen is located within the last 50 amino acids of CLDN1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

PPM1D antibody is human, mouse and rat reactive. At least two isoforms of PPM1D are known to exist; this antibody will only detect the larger isoform.

Reactive Species

A01585-1 is reactive to CLDN1 in Human, Rat

Applications

A01585-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

22.744kDa

Background of Claudin-1

Claudin1 (CLDN1), a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Tight junctions are specialized regions of cell to cell contact consisting of networking strands that act as a molecular gasket for preventing the leakage of ions, water, etc., between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition and CLDN1 expression is often cell type and tissue dependent. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. CLDN1 and CLDN2 were found to be overexpressed in colonal cancer tissues and may be useful as tumor markers and targets for the treatment of colorectal cancer. Characterization of Claudins expression in human tumors can be an additional diagnostic tool. Recent studies show that CLDN1 has gastric tumor suppressive activity and is a direct transcriptional target of RUNX3. Along with SCARB1, LDL-R, and the tetraspanin superfamily member CD81, CLDN1 has been reported to be an entry factor for the Hepatitis C virus.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1-2 μg/mL; ICC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: CLDN1, A01585-1 (1 μg/mL), CLDN1, (2 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Western Blot Validation in Human HepG2 Cell Lysate
Loading: 15 μg of lysates per lane.Antibodies: CLDN1, A01585-1 (A: 1 μg/mL and B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Western Blot Validation in Human Tissue Lysates
Loading: 15 μg of lysates per lane. Antibodies: CLDN1, A01585-1 (1 μg/mL) , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Western Blot Validation in Human Tissue Lysates
Loading: 15 μg of lysates per lane. Antibodies: CLDN1

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Immunofluorescence Validation of CLDN1 in HepG2 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling CLDN1 with A01585-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Gene/Protein Information For CLDN1 (Source: Uniprot.org, NCBI)

Alternative Names

claudin 1; Claudin1; Claudin-1; CLD1; CLDN1; ILVASCSenescence-associated epithelial membrane protein; SEMP1; SEMP1senescence-associated epithelial membrane protein 1 CLDN1 CLD1, ILVASC, SEMP1 claudin 1 claudin-1|senescence-associated epithelial membrane protein 1

For more info on CLDN1, check out the CLDN1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CLDN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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