Product Info Summary
SKU: | FEK0755 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Assay Time: | 120 Minutes |
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Product info
Introduction
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Product Name
Human EPCAM/Trop1 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0755
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human EPCAM/Trop1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human EPCAM in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human EPCAM/Trop1 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0755)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q24 - K265
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0755 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0755 is reactive to EPCAM in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
FEK0755 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of EpCAM/TROP1
Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. Also it is involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since it is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.
Kit Components
Catalog number | Description | Quantity |
---|---|---|
FEK0755-CAP | 96-well plate precoated with anti-Human EpCAM/TROP1 antibody | 1 |
EK0755-ST | lyophilized recombinant Human EpCAM/TROP1 standard | 10 ng/tube |
FEK0755-DA | Biotinylated anti-Human EpCAM/TROP1 detection antibody (50x) | 100 ul |
- | Avidin-Biotin-Peroxidase Complex (30x) | 400 µl |
AR1106-1 | Sample diluent | 30 ml |
AR1106-2 | Antibody diluent | 12 ml |
- | Avidin-Biotin-Peroxidase Diluent | 12 ml |
AR1106-5 | Wash Buffer (25x) | 20 ml |
AR1104 | Color Developing Reagent (TMB) | 10 ml |
AR1105 | Stop Solution | 10 ml |
PLA-SEA | Plate Sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.024 | 0.288 | 0.433 | 0.647 | 1.088 | 1.501 | 2.024 | 2.689 |
Data Example Images
Click image to see more details
Human EPCAM PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 38 | 143 | 444 | 35 | 150 | 444 |
Standard deviation | 1.63 | 6 | 33.74 | 1.82 | 7.5 | 37.29 |
CV (%) | 4.3% | 4.2% | 7.6% | 5.2% | 5% | 8.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of EpCAM/TROP1 in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 38 | 35 | 32 | 35 | 35 | 2.12 | 6% |
Sample 2 | 143 | 161 | 140 | 157 | 150 | 8.92 | 5.9% |
Sample 3 | 444 | 418 | 452 | 413 | 431 | 16.58 | 3.8% |
Protein Target Info & Infographic
Gene/Protein Information For EPCAM (Source: Uniprot.Org, NCBI)
Uniprot ID
P16422
Gene ID
4072
Gene Name
EPCAM
Full Name
Epithelial cell adhesion molecule
Weight
34.932kDa
Superfamily
EPCAM family
Alternative Names
17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A EPCAM DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1 epithelial cell adhesion molecule epithelial cell adhesion molecule|adenocarcinoma-associated antigen|cell surface glycoprotein Trop-1|epithelial glycoprotein 314|human epithelial glycoprotein-2|major gastrointestinal tumor-associated protein GA733-2|membrane component, chromosome 4, surface marker (35kD glycoprotein)|trophoblast cell surface antigen 1|tumor-associated calcium signal transducer 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on EPCAM, check out the EPCAM Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for EPCAM: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human EPCAM/Trop1 PicoKine® Quick ELISA Kit (FEK0755)
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Customer Q&As
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2 Customer Q&As for Human EPCAM/Trop1 PicoKine® Quick ELISA Kit
Question
Dear Support: May I know the storage buffer of this antibody Human EPCAM/Trop1 PicoKine
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
The storage buffer of this antibody FEK0755; peptide is stored in TK Buffer (30mM HEPES, 150mM NaCl, 2mM EDTA, 0.001% Tween-20 and pH 6.75)
Boster Scientific Support
Answered: 2020-01-10
Question
I would like to know the molecular weight of this antibody FEK0755. I checked the datasheet and it states that the observed band size is 35kDa, is these the value of the light chain or heavy chain or the whole antibody? As I know in textbooks it is about 150kDa.
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
The molecular weight for FEK0755 antibody is 160kDa (two light chains: 23-25 kDa each; two heavy chains; 53kDa each). The observed band size of 35kDa in the datasheets refers to the molecular weight of the EpCAM protein detected by the antibody FEK0755.
Boster Scientific Support
Answered: 2019-11-26