Human EPCAM/Trop1 PicoKine® Quick ELISA Kit

EpCAM/TROP1 ELISA kit, 120-minute protocol

Human EPCAM/Trop1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human EPCAM in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: FEK0755
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay Time: 120 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human EPCAM/Trop1 PicoKine® Quick ELISA Kit

See all EpCAM/TROP1 products

SKU/Catalog Number

FEK0755

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human EPCAM/Trop1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human EPCAM in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human EPCAM/Trop1 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0755)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q24 - K265

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0755 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0755 is reactive to EPCAM in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK0755 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of EpCAM/TROP1

Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. Also it is involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since it is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.

Kit Components

Catalog number Description Quantity
FEK0755-CAP 96-well plate precoated with anti-Human EpCAM/TROP1 antibody 1
EK0755-ST lyophilized recombinant Human EpCAM/TROP1 standard 10 ng/tube
FEK0755-DA Biotinylated anti-Human EpCAM/TROP1 detection antibody (50x) 100 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0240.2880.4330.6471.0881.5012.0242.689

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3814344435150444
Standard deviation1.63633.741.827.537.29
CV (%)4.3%4.2%7.6%5.2%5%8.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of EpCAM/TROP1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 138353235352.126%
Sample 21431611401571508.925.9%
Sample 344441845241343116.583.8%
*number of samples for each test n=16.

Gene/Protein Information For EPCAM (Source: Uniprot.Org, NCBI)

Uniprot ID

P16422

Gene ID

4072

Gene Name

EPCAM

Full Name

Epithelial cell adhesion molecule

Weight

34.932kDa

Superfamily

EPCAM family

Alternative Names

17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A EPCAM DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1 epithelial cell adhesion molecule epithelial cell adhesion molecule|adenocarcinoma-associated antigen|cell surface glycoprotein Trop-1|epithelial glycoprotein 314|human epithelial glycoprotein-2|major gastrointestinal tumor-associated protein GA733-2|membrane component, chromosome 4, surface marker (35kD glycoprotein)|trophoblast cell surface antigen 1|tumor-associated calcium signal transducer 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on EPCAM, check out the EPCAM Infographic

EPCAM infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for EPCAM: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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2 Customer Q&As for Human EPCAM/Trop1 PicoKine® Quick ELISA Kit

Question

Dear Support: May I know the storage buffer of this antibody Human EPCAM/Trop1 PicoKine

Verified Customer

Verified customer

Asked: 2020-01-10

Answer

The storage buffer of this antibody FEK0755; peptide is stored in TK Buffer (30mM HEPES, 150mM NaCl, 2mM EDTA, 0.001% Tween-20 and pH 6.75)

Boster Scientific Support

Answered: 2020-01-10

Question

I would like to know the molecular weight of this antibody FEK0755. I checked the datasheet and it states that the observed band size is 35kDa, is these the value of the light chain or heavy chain or the whole antibody? As I know in textbooks it is about 150kDa.

Verified Customer

Verified customer

Asked: 2019-11-26

Answer

The molecular weight for FEK0755 antibody is 160kDa (two light chains: 23-25 kDa each; two heavy chains; 53kDa each). The observed band size of 35kDa in the datasheets refers to the molecular weight of the EpCAM protein detected by the antibody FEK0755.

Boster Scientific Support

Answered: 2019-11-26

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