Anti-MMP14 Antibody Picoband™

MMP-14/MT1-MMP antibody

Boster Bio Anti-MMP14 Antibody Picoband™ catalog # PB9283. Tested in WB applications. This antibody reacts with Human. Cited in 9 publication(s).

Product Info Summary

SKU: PB9283
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-MMP14 Antibody Picoband™

View all MMP-14/MT1-MMP Antibodies

SKU/Catalog Number

PB9283

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MMP14 Antibody Picoband™ catalog # PB9283. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MMP14 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9283)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MMP14, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9283 is reactive to MMP14 in Human

Applications

PB9283 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

65.894kDa

Background of MMP-14/MT1-MMP

Matrix metalloproteinase-14, also called MT-MMP 1, is an enzyme that in humans is encoded by the MMP14 gene. The protein encoded by this gene is a member of the membrane-type MMP subfamily. This gene is mapped to 14q11.2. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. This gene may be involved in actin cytoskeleton reorganization by cleaving PTK7. It acts as a positive regulator of cell growth and migration via activation of MMP15.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For MMP14 (Source: Uniprot.org, NCBI)

Gene Name

MMP14

Full Name

Matrix metalloproteinase-14

Weight

65.894kDa

Superfamily

peptidase M10A family

Alternative Names

EC 3.4.24; EC 3.4.24.80; matrix metallopeptidase 14 (membrane-inserted); matrix metalloproteinase 14 (membrane-inserted); matrix metalloproteinase-14; membrane type 1 metalloprotease; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP14; MMP-14; MMP-X1; MT1MMP; MT1-MMP; MT1-MMPMTMMP1; MT-MMP 1; MT-MMP1 MMP14 MMP-14, MMP-X1, MT-MMP, MT-MMP 1, MT1-MMP, MT1MMP, MTMMP1, WNCHRS matrix metallopeptidase 14 matrix metalloproteinase-14|matrix metallopeptidase 14 (membrane-inserted)|membrane type 1 metalloprotease|membrane-type-1 matrix metalloproteinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MMP14, check out the MMP14 Infographic

MMP14 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MMP14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9283 has been cited in 9 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway

The inhibition of calpains ameliorates vascular restenosis through MMP2/TGF-?1 pathway

Zhang Jt, Sun W, Zhang Wz, Ge Cy, Liu Zy, Zhao Zm, Lu Xs, Fan Yz. Bmc Cancer. 2014 Mar 15;14:193. Doi: 10.1186/1471-2407-14-193. Norcantharidin Inhibits Tumor Growth And Vasculogenic Mimicry Of Human Gallbladder Carcinomas By Suppression Of The Pi...

Zhou H, Wu A, Fu W, Lv Z, Zhang Z. Oncol Lett. 2014 May;7(5):1395-1400. Epub 2014 Feb 27. Significance Of Semaphorin-3A And Mmp-14 Protein Expression In Non-Small Cell Lung Cancer.

Dong Q, Yu D, Yang Cm, Jiang B, Zhang H. Int J Exp Pathol. 2010 Aug;91(4):368-73. Doi: 10.1111/J.1365-2613.2010.00724.X. Epub 2010 Jun 24. Expression Of The Reversion-Inducing Cysteine-Rich Protein With Kazal Motifs And Matrix Metalloproteinase-14...

Shi Ch, Wang Ws, Zhang Zd, Li Cj, Guo Fj, Li F, Chen Am. J Huazhong Univ Sci Technolog Med Sci. 2015 Feb;35(1):111-6. Doi: 10.1007/S11596-015-1398-1. Epub 2015 Feb 12. Effect Of Lentivirus-Mediated Upa Silencing On The Proliferation And Apoptosis ...

Xiao H, Li H, Yu G, Xiao W, Hu J, Tang K, Zeng J, He W, Zeng G, Ye Z, Xu H. Oncol Rep. 2014 Apr;31(4):1832-8. Doi: 10.3892/Or.2014.3048. Epub 2014 Feb 24. Microrna-10B Promotes Migration And Invasion Through Klf4 And Hoxd10 In Human Bladder Cancer.

Jin F, Jiang S, Yang D, Zhang X, Yang Y, Zhang Y, Li K, Yang Y, Ma S. Biochem Biophys Res Commun. 2012 Nov 9;428(1):86-92. Doi: 10.1016/J.Bbrc.2012.10.011. Epub 2012 Oct 8. Acipimox Attenuates Atherosclerosis And Enhances Plaque Stability In Apoe-...

Jiang S, Jin F, Li D, Zhang X, Yang Y, Yang D, Li K, Yang Y, Ma S. High Alt Med Biol. 2013 Jun;14(2):175-80. Doi: 10.1089/Ham.2012.1083. Intermittent Hypobaric Hypoxia Promotes Atherosclerotic Plaque Instability In Apoe-Deficient Mice.

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15 Customer Q&As for Anti-MMP14 Antibody Picoband™

Question

Do you have a BSA free version of anti-MMP14 antibody PB9283 available?

Verified Customer

Verified customer

Asked: 2020-04-28

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MMP14 antibody PB9283 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-28

Question

We are currently using anti-MMP14 antibody PB9283 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-03

Answer

The anti-MMP14 antibody (PB9283) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-03

Question

Our team were happy with the WB result of your anti-MMP14 antibody. However we have observed positive staining in skin membrane using this antibody. Is that expected? Could you tell me where is MMP14 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-02-27

Answer

According to literature, skin does express MMP14. Generally MMP14 expresses in membrane. Regarding which tissues have MMP14 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 7649159
Melanoma, Pubmed ID: 17081065
Placenta, Pubmed ID: 7721107, 8015608
Skin, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-02-27

Question

We have seen staining in human tongue. Are there any suggestions? Is anti-MMP14 antibody supposed to stain tongue positively?

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

From what I have seen in literature tongue does express MMP14. From what I have seen in Uniprot.org, MMP14 is expressed in endocervix, placenta, lung, tongue, skin, melanoma, among other tissues. Regarding which tissues have MMP14 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 7649159
Melanoma, Pubmed ID: 17081065
Placenta, Pubmed ID: 7721107, 8015608
Skin, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-02-26

Question

I was wanting to use your anti-MMP14 antibody for WB for human skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human skin identification?

Verified Customer

Verified customer

Asked: 2020-02-03

Answer

It shows on the product datasheet, PB9283 anti-MMP14 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human skin in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-03

Question

I am interested in using your anti-MMP14 antibody for branching morphogenesis of an epithelial tube studies. Has this antibody been tested with western blotting on human placenta tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-04

Answer

I appreciate your inquiry. This PB9283 anti-MMP14 antibody is validated on human placenta tissue, tissue lysate, hela whole cell lysate, u87 whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-04

Question

I see that the anti-MMP14 antibody PB9283 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-12

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-12

Question

I have a question about product PB9283, anti-MMP14 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9283 anti-MMP14 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-24

Question

Is this PB9283 anti-MMP14 antibody reactive to the isotypes of MMP14?

J. Taylor

Verified customer

Asked: 2019-02-12

Answer

The immunogen of PB9283 anti-MMP14 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human MMP14 (557-582aa LAVFFFRRHGTPRRLLYCQRSLLDKV), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-02-12

Question

Please see the WB image, lot number and protocol we used for skin using anti-MMP14 antibody PB9283. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-08-15

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-08-15

Question

I would like to test anti-MMP14 antibody PB9283 on human skin for research purposes, then I may be interested in using anti-MMP14 antibody PB9283 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-11-27

Answer

The products we sell, including anti-MMP14 antibody PB9283, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-11-27

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-MMP14 antibody PB9283. Let me know if you need anything else.

K. Thomas

Verified customer

Asked: 2017-11-13

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-11-13

Question

Is a blocking peptide available for product anti-MMP14 antibody (PB9283)?

Verified Customer

Verified customer

Asked: 2017-08-29

Answer

We do provide the blocking peptide for product anti-MMP14 antibody (PB9283). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-08-29

Question

Would anti-MMP14 antibody PB9283 work for WB with skin?

Verified Customer

Verified customer

Asked: 2017-06-29

Answer

According to the expression profile of skin, MMP14 is highly expressed in skin. So, it is likely that anti-MMP14 antibody PB9283 will work for WB with skin.

Boster Scientific Support

Answered: 2017-06-29

Question

Does PB9283 anti-MMP14 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

S. Singh

Verified customer

Asked: 2016-11-15

Answer

It shows on the product datasheet, PB9283 anti-MMP14 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-11-15

Order DetailsPrice
PB9283

100μg

$370
PB9283-10ug

10μg sample (liquid)

$99
PB9283-Biotin

100 μg Biotin conjugated

$570
PB9283-Cy3

100 μg Cy3 conjugated

$570
PB9283-Dylight488

100 μg Dylight488 conjugated

$570
PB9283-Dylight550

100 μg Dylight550 conjugated

$570
PB9283-Dylight594

100 μg Dylight594 conjugated

$570
PB9283-FITC

100 μg FITC conjugated

$570
PB9283-HRP

100 μg HRP conjugated

$570
PB9283-APC

100 μg APC conjugated

$670
PB9283-PE

100 μg PE conjugated

$670
PB9283-carrier-free

Carrier Free

$370

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