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Product Info Summary
| SKU: | M00656 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MMP14/Mt1 Mmp Rabbit Monoclonal Antibody
SKU/Catalog Number
M00656
BM4119 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MMP14/Mt1 Mmp Rabbit Monoclonal Antibody catalog # M00656. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MMP14/Mt1 Mmp Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00656)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BFD-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MMP14
Reactive Species
M00656 is reactive to MMP14 in Human, Mouse, Rat
Observed Molecular Weight
55 kDa
Calculated molecular weight
65.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00656 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: human placenta tissue, rat kidney tissue, rat heart tissue, mouse kidney tissue, mouse heart tissue
IHC: human kdieny tissue
Validation Images & Assay Conditions
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Western blot analysis of MT1-MMP/MMP14 using anti-MT1-MMP/MMP14 antibody (M00656).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MT1-MMP/MMP14 antigen affinity purified monoclonal antibody (M00656) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MT1-MMP/MMP14 at approximately 55 kDa. The expected band size for MT1-MMP/MMP14 is at 66 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human kdieny, using MMP14 Antibody .
Click image to see more details
IF analysis of MMP14 using anti-MMP14 antibody (M00656).
MMP14 was detected in an immunocytochemical section of Mouse cell culture coverslip. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1:200 rabbit anti-CBL Antibody (PB9077) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a laser confocol.
Specific Publications For Anti-MMP14/Mt1 Mmp Rabbit Monoclonal Antibody (M00656)
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Customer Reviews
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1 Reviews For Anti-MMP14/Mt1 Mmp Rabbit Monoclonal Antibody
Highly prone to positive signals, with precise expression localization
Excellent
| SKU | M00656 |
|---|---|
| Application | Immunofluorenscence |
| Sample | Mouse MC-8 cells |
| Sample Processing Description | 4% paraformaldehyde for 15 minutes |
| Primary Antibody | Anti-MMP14/Mt1 Mmp Rabbit Monoclonal Antibody |
| Primary Incubation | 1:200, overnight at 4 ℃ |
| Blocking Agent | Goat serum |
| Secondary Antibody | DyLight 488-conjugated Goat Anti-Rabbit IgG. |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Laser confocal microscopy |
| Results Summary | I will purchase Boster products again and recommend them to my classmates and colleagues. |
Guangyu Mei, Tongji University
Verified customer
Submitted 2025-10-17
Customer Q&As
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16 Customer Q&As for Anti-MMP14/Mt1 Mmp Rabbit Monoclonal Antibody
Question
Will anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 work for IHC with skin?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
According to the expression profile of skin, MMP14 is highly expressed in skin. So, it is likely that anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 will work for IHC with skin.
Boster Scientific Support
Answered: 2020-04-28
Question
I see that the anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-03-30
Question
Is a blocking peptide available for product anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody (M00656)?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
We do provide the blocking peptide for product anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody (M00656). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-18
Question
I have a question about product M00656, anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00656 anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-28
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-12
Question
Do you have a BSA free version of anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 available?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-27
Question
We have seen staining in mouse lung. Any tips? Is anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2018-12-06
Answer
Based on literature lung does express MMP14. Based on Uniprot.org, MMP14 is expressed in endocervix, placenta, lung, tongue, skin, melanoma, among other tissues. Regarding which tissues have MMP14 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 7649159
Melanoma, Pubmed ID: 17081065
Placenta, Pubmed ID: 7721107, 8015608
Skin, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-12-06
Question
Is this M00656 anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody reactive to the isotypes of MMP14?
S. Brown
Verified customer
Asked: 2018-09-24
Answer
The immunogen of M00656 anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody is A synthesized peptide derived from human MMP14 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-24
Question
See below the WB image, lot number and protocol we used for skin using anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656. Please let me know if you require anything else.
P. Johnson
Verified customer
Asked: 2018-08-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-27
Question
Would anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 work on feline IF with placenta?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
Our lab technicians have not tested anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 on feline. You can run a BLAST between feline and the immunogen sequence of anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline placenta in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-02
Question
We are interested in to test anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 on human skin for research purposes, then I may be interested in using anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-02-23
Answer
The products we sell, including anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-23
Question
Will M00656 anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Roberts
Verified customer
Asked: 2017-07-20
Answer
It shows on the product datasheet, M00656 anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-20
Question
We are currently using anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
J. Jones
Verified customer
Asked: 2017-04-13
Answer
The anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody (M00656) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-04-13
Question
Our lab were well pleased with the WB result of your anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody. However we have seen positive staining in endocervix membrane using this antibody. Is that expected? Could you tell me where is MMP14 supposed to be expressed?
O. Wu
Verified customer
Asked: 2015-05-07
Answer
From what I have seen in literature, endocervix does express MMP14. Generally MMP14 expresses in membrane. Regarding which tissues have MMP14 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 7649159
Melanoma, Pubmed ID: 17081065
Placenta, Pubmed ID: 7721107, 8015608
Skin, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-05-07
Question
We have tried in the past anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody for IP on tongue in the past. I am using human, and We are going to use the antibody for IHC next. you antibody examining tongue as well as melanoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
S. Dhar
Verified customer
Asked: 2014-07-24
Answer
I viewed the website and datasheets of our anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody and it seems that M00656 has been validated on human in both IP and IHC. Thus M00656 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-07-24
Question
I was wanting to use your anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody for IHC for human skin on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human skin identification?
Z. Walker
Verified customer
Asked: 2013-09-18
Answer
As indicated on the product datasheet, M00656 anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody has been validated for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-09-18
