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MMP2 antibody (A00286-2) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 87 Publications

a00286 2 mmp2 primary antibodies fcm testing 5

Flow Cytometry analysis of U87 cells using anti-MMP2 antibody (A00286-2).
Overlay histogram showing U87 cells stained with A00286-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MMP2 Antibody (A00286-2, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-MMP2 Antibody Picoband®

MMP2 antibody

87 Publications

Reactive Species	Host	Application
Human, Rat	Rabbit	ELISA, Flow Cytometry, IHC, WB

Tested Applications

MMP2 antibody was positively detected by WB in:

human U87 whole cell, rat lung tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

MMP2 antibody was positively detected by IHC in:

human mammary cancer tissue, human prostatic cancer tissue, human prostatic cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

MMP2 antibody was positively detected by FC in:

U87 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Human

Tested

Recommend

Rat

Tested

Recommend

Monkey

Predicted

Mouse

Predicted

Dog

Predicted

Sheep

Predicted

Bovine

Predicted

Rabbit

Predicted

Product Information

Product Name

Anti-MMP2 Antibody Picoband®
View all MMP2 Antibodies

Form

Lyophilized

Uniprot

P08253

Gene ID

4313

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MMP2 recombinant protein (Position: L411-C660).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

72 kDa

Calculated Molecular Weight

73.9 kDa

Background of MMP2

Matrix metalloproteinase-2 (MMP2) is a Type IV collagenase, 72-kD, which is also known as gelatinase and is a member of a group of secreted zinc metalloproteases. The MMP2 gene is 17 kb long with 13 exons varying in size from 110 to 901 bp and 12 introns ranging from 175 to 4,350 bp, located within a region of human chromosome 16q13. In addition, the extra exons encode the amino acids of the fibronectin-like domain which has so far been found in only the 72- and 92-kDa type IV collagenase. MMP2, which has a critical role in the binding of progelatinase A and TIMP4 via the C-terminal hemopexin-like domain (C domain), is functionally associated on the surface of angiogenic blood vessels. Not only is a likely effector of endometrial menstrual breakdown, MMP2 is also effector and regulator of the inflammatory response. Moreover, MMP2 could be helpful in diagnosing Takayasu arteritis.

Cite This Product

Anti-MMP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00286-2)

Protocols

Western Blot protocol for MMP2 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for MMP2 antibody

Review Protocol

Flow Cytometry (FC) protocol for MMP2 antibody

Review Protocol

Publications

Macrophages orchestrate tail regeneration in lizards Scincella tsinlingensis through spatiotemporal control of immunity, oxidative stress, and myogenesis

Published: 2026-08-06

Journal: Frontiers in Cell and Developmental Biology

Application: IF

Impact Factor: 5.30

Species: Lizards

PMID: 42625655

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Free Secondary Antibody

Anti-MMP2 Antibody Picoband®

MMP2 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Macrophages orchestrate tail regeneration in lizards Scincella tsinlingensis through spatiotemporal control of immunity, oxidative stress, and myogenesis

Dioscin Suppresses Ovarian and Gastric Tumor Progression by Inhibiting ALDH1A3-Mediated Retinoic Acid Metabolism and Cancer Stemness

tRF-AspGTC Promotes Intracranial Aneurysm Formation by Controlling TRIM29-Mediated Galectin-3 Ubiquitination

Morinda officinalis iridoid glycosides, as an inhibitor of GSK-3β, alleviates rheumatoid arthritis through inhibition of NF-κB and JAK2/STAT3 pathway

Intracranial aneurysm circulating exosome-derived LncRNA ATP1A1-AS1 promotes smooth muscle cells phenotype switching and apoptosis

Trophoblast cellular adhesion molecule expression regulated by different genes and their correlation with pregnancy-induced hypertension

Comprehensive Analysis of Sideroflexin 4 in Hepatocellular Carcinoma by Bioinformatics and Experiments

Single and Combined Effects of Short-Term Selenium Deficiency and T-2 Toxin-Induced Kidney Pathological Injury Through the MMPs/TIMPs System

Licoflavone A Suppresses Gastric Cancer Growth and Metastasis by Blocking the VEGFR-2 Signaling Pathway

Hydrogen sulfide ameliorates doxorubicin‑induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway

Long Noncoding RNA TINCR-Mediated Regulation of Acetyl-CoA Metabolism Promotes Nasopharyngeal Carcinoma Progression and Chemoresistance

MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88

HMGB1 promoted P-glycoprotein at the blood-brain barrier in MCAO rats via TLR4/NF-κB signaling pathway

MiR-296-3p may affect the proliferation and migration of non-small cell lung cancer cells via regulating RABL3

Antitumor evaluation of carboxymethyl chitosan based norcantharidin conjugates against gastric cancer as novel polymer therapeutics

Employing the Sirolimus-Eluting Poly (Propylene Carbonate) Mesh for the Prevention of Arteriovenous Graft Stenosis in Rats

Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels

Involvement of S100A4/Mts1 and associated proteins in the protective effect of fluoxetine against MCT – Induced pulmonary hypertension in rats

Multilayered coating of titanium implants promotes coupled osteogenesis and angiogenesis in vitro and in vivo

RRM2 promotes the progression of human glioblastoma

Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells

Prophylactic effects of triptolide on colon cancer development in azoxymethane/dextran sulfate sodiuminduced mouse model

Ursolic acid ameliorates oxidative stress, inflammation and fibrosis in diabetic cardiomyopathy rats

Main components of pomegranate, ellagic acid and luteolin, inhibit metastasis of ovarian cancer by down-regulating MMP2 and MMP9

Cardioprotection of Sheng Mai Yin a classic formula on adriamycin induced myocardial injury in Wistar rats

DEK promoted EMT and angiogenesis through regulating PI3K/AKT/mTOR pathway in triple-negative breast cancer

Effects of baicalein on proliferation, apoptosis, migration and invasion of Ewing's sarcoma cells

Enhancing E-cadherin expression via promoter-targeted miR-373 suppresses bladder cancer cells growth and metastasis

Kallikrein-related peptidase 4 contributes to the tumor metastasis of oral squamous cell carcinoma

PAD1 promotes epithelial-mesenchymal transition and metastasis in triple-negative breast cancer cells by regulating MEK1-ERK1/2-MMP2 signaling

Tetrahydrocurcumin induces mesenchymal-epithelial transition and suppresses angiogenesis by targeting HIF-1α and autophagy in human osteosarcoma

Vimentin is a crucial target for anti-metastasis therapy of nasopharyngeal carcinoma

Overexpression of RASD1 inhibits glioma cell migration/invasion and inactivates the AKT/mTOR signaling pathway

Inhibition of glioblastoma growth and invasion by 125I brachytherapy in rat glioma model

Short interfering RNA targeting Net1 reduces the angiogenesis and tumor growth of in vivo cervical squamous cell carcinoma through VEGF down-regulation

In vivo inhibitory activity of andrographolide derivative ADN-9 against liver cancer and its mechanisms involved in inhibition of tumor angiogenesis

Silencing of astrocyte elevated gene-1 inhibits proliferation and migration of melanoma cells and induces apoptosis

Silencing of Y-box binding protein-1 by RNA interference inhibits proliferation, invasion, and metastasis, and enhances sensitivity to cisplatin through NF-κB signaling pathway in human neuroblastoma SH-SY5Y cells

Effect of crocetin on vascular smooth muscle cells migration induced by advanced glycosylation end products

The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment

miR-320a functions as a suppressor for gliomas by targeting SND1 and β-catenin, and predicts the prognosis of patients

The expression and significance of IL-6, IFN-γ, SM22α, and MMP-2 in rat model of aortic dissection.

Circular RNA 0000096 affects cell growth and migration in gastric cancer

Long-term intravenous administration of carboxylated single-walled carbon nanotubes induces persistent accumulation in the lungs and pulmonary fibrosis via the nuclear factor-kappa B pathway

Lichong decoction reduces Matrix Metalloproteinases-2 expression but increases Tissue Inhibitors of Matrix Metalloproteinases-2 expression in a rat model of uterine leiomyoma

Silencing of ATPase family AAA domain-containing protein 2 inhibits migration and invasion of colorectal cancer cells.

TGF-β1 promotes scar fibroblasts proliferation and transdifferentiation via up-regulating MicroRNA-21

Effects of electromagnetic pulse exposure on gelatinase of blood–brain barrier in vitro

Knockdown of placental growth factor (PLGF) mitigates hyperoxia-induced acute lung injury in neonatal rats: Suppressive effects on NFκB signaling pathway

SPOCK1 promotes the proliferation, migration and invasion of glioma cells through PI3K/AKT and Wnt/β-catenin signaling pathways

NOB1 silencing inhibits the growth and metastasis of laryngeal cancer cells through the regulation of JNK signaling pathway

Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial–mesenchymal transition via NF-κB activation

Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-κB Pathway

Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC‑α/ERK1/2 pathway: An in vitro investigation Corrigendum in /10.3892/mmr.2016.4976

Amniotic Mesenchymal Stem Cells Can Enhance Angiogenic Capacity via MMPs In Vitro and In Vivo

Effect of Ginkgo biloba extract on experimental cardiac remodeling

Shikonin inhibits the cell viability, adhesion, invasion and migration of the human gastric cancer cell line MGC-803 via the Toll-like receptor 2/nuclear factor-kappa B pathway

Afatinib inhibits proliferation and invasion and promotes apoptosis of the T24 bladder cancer cell line

Elevated expression levels of androgen receptors and matrix metalloproteinase-2 and -9 in 30 cases of hepatocellular carcinoma compared with adjacent tissues as predictors of cancer invasion and staging

Norcantharidin enhances TIMP‑2 anti‑vasculogenic mimicry activity for human gallbladder cancers through downregulating MMP‑2 and MT1‑MMP

Astragaloside IV inhibits migration and invasion in human lung cancer A549 cells via regulating PKC-α-ERK1/2-NF-κB pathway

Correlations of lysyl oxidase with MMP2/MMP9 expression and its prognostic value in non-small cell lung cancer

Morroniside Improves Microvascular Functional Integrity of the Neurovascular Unit after Cerebral Ischemia

Clinical significance of integrin β6 as a tumor recurrence factor in follicular thyroid carcinoma

Expression of the tissue inhibitor of metalloproteinase-3 by transplanted VSMCs modifies heart structure and function after myocardial infarction

Correlation between matrix metalloproteinase expression and activation of the focal adhesion kinase signaling pathway in herpes stromal keratitis

In vitro and in vivo study of dolichyl phosphate on the efflux activity of P-glycoprotein at the blood–brain barrier