Anti-MMP2 Antibody Picoband®

Western blot analysis of MMP2 using anti-MMP2 antibody (A00286-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: rat lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP2 antigen affinity purified polyclonal antibody (Catalog # A00286-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP2 at approximately 72KD. The expected band size for MMP2 is at 72KD.

IHC analysis of MMP2 using anti-MMP2 antibody (A00286-2).
MMP2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MMP2 Antibody (A00286-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

IHC analysis of MMP2 using anti-MMP2 antibody (A00286-2).
MMP2 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MMP2 Antibody (A00286-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

IHC analysis of MMP2 using anti-MMP2 antibody (A00286-2).
MMP2 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MMP2 Antibody (A00286-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Flow Cytometry analysis of U87 cells using anti-MMP2 antibody (A00286-2).
Overlay histogram showing U87 cells stained with A00286-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MMP2 Antibody (A00286-2, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Overexpression of RASD1 inhibits the invasion ability of glioma cells. ( A ) A transwell invasion assay was performed in U251 and U87 cells. Overexpression of RASD1 significantly decreased the number of cells that passed through the Matrigel. Scale bar: 100 μm; *** P < 0.001. ( B ) MMP2 Western blot analysis was employed to reflect the invasion ability of U251 and U87 cells. Overexpression of RASD1 significantly decreased the protein levels of MMP2. * P < 0.05; *** P < 0.001.
Index in PubMed under a CC BY license. PMID: 28600528

SFXN4 knockdown inhibits HCC migration and invasion. (A-B) Transwell assays were conducted to measure migration and invasion of Huh7 (A) and MHCC97H (B). (C-D) The qRT-PCR and Western blot were performed to detect MMP2 mRNA (C) and protein (D) expression in indicated cells. *** P <0.001, ** P <0.01. Scale bars: 100 μm.
Index in PubMed under a CC BY license. PMID: 37786439

Effects of MOIG on adhesion, migration, invasion and the expression of associated proteins of TNF-α-stimulated FLSs cells. (A) Adhesion of FLSs cells. (B) and (C) The wound healing of FLSs at 24 and 48 h; (D) and (F) The migration of FLSs at 6 h; (E) and (G) The invasion of FLSs at 24 h; ( H–M ) the expression of ICAM-1, VCAM-1, cadherin 11, MMP2 and MMP3 of FLSs. The data were expressed as mean ± SD (n = 3). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. normal ctrl group; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. TNF-α model group.
Index in PubMed under a CC BY license. PMID: 39444614