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Product Info Summary
| SKU: | A04079 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CHD2 Antibody Picoband®
SKU/Catalog Number
A04079
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CHD2 Antibody Picoband® catalog # A04079. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CHD2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04079)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CHD2 recombinant protein (Position: R1124-K1351). Human CHD2 shares 97.4% amino acid (aa) sequence identity with mouse CHD2.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04079 is reactive to CHD2 in Human, Monkey, Mouse, Rat
Observed Molecular Weight
250 kDa
Calculated molecular weight
211.3 kDa
Background of CHD2
Chromodomain-helicase-DNA-binding protein 2 is an enzyme that in humans is encoded by the CHD2 gene. The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. CHD2 catalyzes the assembly of chromatin into periodic arrays; and the N-terminal region of CHD2, which contains tandem chromodomains, serves an auto-inhibitory role in both the DNA-binding and ATPase activities of CHD2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04079 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat
Positive Control
WB: monkey COS-7 whole cell, human Siha whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
IHC: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of CHD2 using anti-CHD2 antibody (A04079).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: monkey COS-7 whole cell lysates,
Lane 2: human Siha whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHD2 antigen affinity purified polyclonal antibody (Catalog # A04079) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHD2 at approximately 250 kDa. The expected band size for CHD2 is at 211 kDa.
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IHC analysis of CHD2 using anti-CHD2 antibody (A04079).
CHD2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CHD2 Antibody (A04079) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of CHD2 using anti-CHD2 antibody (A04079).
CHD2 was detected in paraffin-embedded section of rat brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CHD2 Antibody (A04079) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of CHD2 using anti-CHD2 antibody (A04079).
CHD2 was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CHD2 Antibody (A04079) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-CHD2 Antibody Picoband® (A04079)
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Customer Q&As
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13 Customer Q&As for Anti-CHD2 Antibody Picoband®
Question
Does A04079 anti-CHD2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
It shows on the product datasheet, A04079 anti-CHD2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-22
Question
Would anti-CHD2 antibody A04079 work for WB with liver?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
According to the expression profile of liver, CHD2 is highly expressed in liver. So, it is likely that anti-CHD2 antibody A04079 will work for WB with liver.
Boster Scientific Support
Answered: 2019-12-03
Question
I see that the anti-CHD2 antibody A04079 works with WB, what is the protocol used to produce the result images on the product page?
T. Zhao
Verified customer
Asked: 2019-11-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-15
Question
Is a blocking peptide available for product anti-CHD2 antibody (A04079)?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
We do provide the blocking peptide for product anti-CHD2 antibody (A04079). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-10-10
Question
Would anti-CHD2 antibody A04079 work on monkey WB with liver?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
Our lab technicians have not validated anti-CHD2 antibody A04079 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-CHD2 antibody A04079 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-30
Question
We are currently using anti-CHD2 antibody A04079 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
The anti-CHD2 antibody (A04079) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-24
Question
I have a question about product A04079, anti-CHD2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04079 anti-CHD2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-24
Question
Here is the WB image, lot number and protocol we used for liver using anti-CHD2 antibody A04079. Please let me know if you require anything else.
F. Zhao
Verified customer
Asked: 2019-02-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-05
Question
Is this A04079 anti-CHD2 antibody reactive to the isotypes of CHD2?
Verified Customer
Verified customer
Asked: 2019-01-07
Answer
The immunogen of A04079 anti-CHD2 antibody is E. coli-derived human CHD2 recombinant protein (Position: R1124-K1351). Human CHD2 shares 97.4% amino acid (aa) sequence identity with mouse CHD2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-07
Question
I was wanting to use to test anti-CHD2 antibody A04079 on rat liver for research purposes, then I may be interested in using anti-CHD2 antibody A04079 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
O. Mangal
Verified customer
Asked: 2018-11-16
Answer
The products we sell, including anti-CHD2 antibody A04079, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-11-16
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CHD2 antibody A04079. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-04-24
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-24
Question
Is there a BSA free version of anti-CHD2 antibody A04079 available?
Verified Customer
Verified customer
Asked: 2018-03-02
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CHD2 antibody A04079 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-02
Question
I was wanting to use your anti-CHD2 antibody for WB for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?
A. Li
Verified customer
Asked: 2018-02-08
Answer
As indicated on the product datasheet, A04079 anti-CHD2 antibody has been tested for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-08
