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Human PDGF-AB ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human PDGF-AB. 96wells/kit, with removable strips. Cited in 1 publication(s).
Human PDGF-AB ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0484 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Human |
| Sample Type: | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <2pg/ml |
| Assay Range: | 31.2pg/ml-2000pg/ml |
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Overview
| Product Name | Human PDGF-AB ELISA Kit PicoKine™
See all PDGFB primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0484 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Human Pdgf Bb/PDGFB with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Pdgf Bb/PDGFB tissue specificity: Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung. . |
| Cite This Product | Human PDGF-AB ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0484) |
Product Specs
| Reactivity/Species | Human |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity | <2pg/ml |
| Assay Range | 31.2pg/ml-2000pg/ml |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: (S87-T211)+(S82-T190) |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P01127 |
|---|---|
| Gene Name | PDGFB |
| Protein Name | Platelet-derived growth factor subunit B,Platelet-derived growth factor subunit A |
| Subcellular Localization | Secreted. Released by platelets upon wounding. |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity). . |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Human PDGF-AB ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0484-CAP | 96-well plate precoated with anti-Human PDGFB antibody | 1 |
| EK0484-ST | lyophilized recombinant Human PDGFB standard | 10ng/tube |
| EK0484-DA | biotinylated anti-Human PDGFB antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0484 Human PDGF-AB ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.023 | 0.126 | 0.252 | 0.423 | 0.922 | 1.358 | 1.856 | 2.195 |
We measured random samples of Human PDGF-AB ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 60 | 259 | 808 | 62 | 271 | 865 |
| Standard deviation | 3.96 | 14.76 | 51.71 | 4.52 | 19.51 | 68.33 |
| CV(%) | 6.6% | 5.7% | 6.4% | 7.3% | 7.2% | 7.9% |
We measured the performance consistency of Human PDGF-AB ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 60 | 69 | 60 | 60 | 62 | 3.89 | 6.2% |
| Sample 2 | 259 | 267 | 273 | 260 | 264 | 5.67 | 2.1% |
| Sample 3 | 808 | 809 | 832 | 839 | 822 | 13.72 | 1.6% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 1
| Takeuchi M, Kamei N, Shinomiya R, Sunagawa T, Suzuki O, Kamoda H, Ohtori S, Ochi M. Neuroreport. 2012 Aug 22;23(12):712-6. Doi: 10.1097/Wnr.0B013E3283567196. Human Platelet-Rich Plasma Promotes Axon Growth In Brain-Spinal Cord Coculture. | PubMed ID 22750774 |
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Shipping Information
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
Contact Information
| [email protected] | |
| (888) 466-3604 |
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