Human PDGF-AB ELISA Kit PicoKine®

Human PDGF-AB ELISA Kit PicoKine™ (96 Tests). Quantitate Human PDGF-AB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. Cited in 5 publication(s).

Product Info Summary

SKU: EK0484
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human PDGF-AB ELISA Kit PicoKine®

SKU/Catalog Number

EK0484

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human PDGF-AB ELISA Kit PicoKine™ (96 Tests). Quantitate Human PDGF-AB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human PDGF-AB ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0484)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: (S87-T211) + (S82-T190)

Sensitivity

<2 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0484 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0484 is reactive to in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0484 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of

The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22. The standard product used in this kit is recombinant human PDGF-AB with the molecular mass of 27KDa.

Kit Components

Catalog Number Description Quantity
EK0484-CAP Anti-Human Pre-coated 96-well strip microplate 1
EK0484-ST Human Standard 2 vials, 10 ng/tube
EK0484-DA Human Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0230.1260.2520.4230.9221.3581.8562.095

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, For serum, dilution ratio is 1:20 and concentration is 5432-34260 pg/ml.
For plasma, dilution ratio is 1:1 and concentration is 34-732 pg/ml..

Some articles we found to cite concentrations of in samples: 8632294 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human PDGF-AB ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)6025980862271865
Standard deviation3.9614.7651.714.5219.5168.33
CV (%)6.6%5.7%6.4%7.3%7.2%7.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 160696060623.896.2%
Sample 22592672732602645.672.1%
Sample 380880983283982213.721.6%
*number of samples for each test n=16.

Hello CJ!

EK0484 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Anti-inflammatory Effect of Curcumin, Homotaurine, and Vitamin D3 on Human Vitreous in Patients With Diabetic Retinopathy

Functional assessment of autologous platelet-rich plasma (PRP) after long-term storage at −20 °C without any preservation agent

Bone intramedullary reaming grafts the fracture site with CD146+ skeletal progenitors and downmodulates the inflammatory environment

Platelet-derived alpha-granules are associated with inflammation in patients with NK/T-cell lymphoma-associated hemophagocytic syndrome

Takeuchi M, Kamei N, Shinomiya R, Sunagawa T, Suzuki O, Kamoda H, Ohtori S, Ochi M. Neuroreport. 2012 Aug 22;23(12):712-6. Doi: 10.1097/Wnr.0B013E3283567196. Human Platelet-Rich Plasma Promotes Axon Growth In Brain-Spinal Cord Coculture.

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9 Customer Q&As for Human PDGF-AB ELISA Kit PicoKine®

Question

Q: What is the optimal O.D. value for PDGFB ELISA kit? I performed your PDGFB ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain PDGFB even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-10-18

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this PDGFB ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of PDGFB that can be declared/interpreted as positive by the above standard is 2pg/ml.

Boster Scientific Support

Answered: 2020-10-18

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification PDGFB using the EK0484 Human PDGFB Picokine® ELISA Kit?

G. Mehta

Verified customer

Asked: 2020-08-15

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2020-08-15

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

G. Phillips

Verified customer

Asked: 2018-05-17

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-05-17

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-04-27

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-04-27

Question

Q: how to proceed with the analysis of ELISA data? I have obtained PDGFB level in serum.

T. Brown

Verified customer

Asked: 2017-09-29

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2017-09-29

Question

Q: is it okay to use citrate plasma as samples in Human PDGFB Picokine® ELISA Kit (Catalog # EK0484)?

J. Taylor

Verified customer

Asked: 2017-07-01

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of PDGFB causing disruption of its protein structure. PDGFB may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the PDGFB ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2017-07-01

Question

Q: if the enzyme conjugated PDGFB antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the PDGFB antigen?

Verified Customer

Verified customer

Asked: 2016-12-18

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-12-18

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

P. Harris

Verified customer

Asked: 2016-06-09

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2016-06-09

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-04-14

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-04-14

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