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Product Info Summary
| SKU: | EK0484 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
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Product info
Product Name
Human PDGF-AB ELISA Kit PicoKine®
SKU/Catalog Number
EK0484
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human PDGF-AB ELISA Kit PicoKine™ (96 Tests). Quantitate Human PDGFB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PDGF-AB ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0484)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: (S87-T211) + (S82-T190)
Sensitivity
<2 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0484 is reactive to PDGFB in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0484 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PDGFB
The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22. The standard product used in this kit is recombinant human PDGF-AB with the molecular mass of 27KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0484-CAP | Anti-Human PDGFB Pre-coated 96-well strip microplate | 1 |
| EK0484-ST | Human PDGFB Standard | 2 vials, 10 ng/tube |
| EK0484-DA | Human PDGFB Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.023 | 0.126 | 0.252 | 0.423 | 0.922 | 1.358 | 1.856 | 2.195 |
Data Example Images
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Human PDGF-AB PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PDGF-AB ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 60 | 259 | 808 | 62 | 271 | 865 |
| Standard deviation | 3.96 | 14.76 | 51.71 | 4.52 | 19.51 | 68.33 |
| CV (%) | 6.6% | 5.7% | 6.4% | 7.3% | 7.2% | 7.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of PDGFB in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 60 | 69 | 60 | 60 | 62 | 3.89 | 6.2% |
| Sample 2 | 259 | 267 | 273 | 260 | 264 | 5.67 | 2.1% |
| Sample 3 | 808 | 809 | 832 | 839 | 822 | 13.72 | 1.6% |
Protein Target Info & Infographic
Gene/Protein Information For PDGFB (Source: Uniprot.Org, NCBI)
Uniprot ID
P01127
Gene ID
5155
Gene Name
PDGFB
Full Name
Platelet-derived growth factor subunit B
Weight
27.283kDa
Superfamily
PDGF/VEGF growth factor family
Alternative Names
PDGFBB; PDGF-BB PDGFB IBGC5, PDGF-2, PDGF2, SIS, SSV, c-sis platelet derived growth factor subunit B platelet-derived growth factor subunit B|PDGF subunit B|PDGF, B chain|becaplermin|epididymis secretory sperm binding protein|platelet-derived growth factor 2|platelet-derived growth factor B chain|platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)|platelet-derived growth factor, beta polypeptide (oncogene SIS)|proto-oncogene c-Sis
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PDGFB, check out the PDGFB Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PDGFB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human PDGF-AB ELISA Kit PicoKine® (EK0484)
EK0484 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Anti-inflammatory Effect of Curcumin, Homotaurine, and Vitamin D3 on Human Vitreous in Patients With Diabetic Retinopathy
Functional assessment of autologous platelet-rich plasma (PRP) after long-term storage at −20 °C without any preservation agent
Bone intramedullary reaming grafts the fracture site with CD146+ skeletal progenitors and downmodulates the inflammatory environment
Platelet-derived alpha-granules are associated with inflammation in patients with NK/T-cell lymphoma-associated hemophagocytic syndrome
Takeuchi M, Kamei N, Shinomiya R, Sunagawa T, Suzuki O, Kamoda H, Ohtori S, Ochi M. Neuroreport. 2012 Aug 22;23(12):712-6. Doi: 10.1097/Wnr.0B013E3283567196. Human Platelet-Rich Plasma Promotes Axon Growth In Brain-Spinal Cord Coculture.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
9 Customer Q&As for Human PDGF-AB ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for PDGFB ELISA kit? I performed your PDGFB ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain PDGFB even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-10-18
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this PDGFB ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of PDGFB that can be declared/interpreted as positive by the above standard is 2pg/ml.
Boster Scientific Support
Answered: 2020-10-18
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification PDGFB using the EK0484 Human PDGFB Picokine® ELISA Kit?
G. Mehta
Verified customer
Asked: 2020-08-15
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2020-08-15
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
G. Phillips
Verified customer
Asked: 2018-05-17
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-05-17
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-04-27
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-04-27
Question
Q: how to proceed with the analysis of ELISA data? I have obtained PDGFB level in serum.
T. Brown
Verified customer
Asked: 2017-09-29
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-09-29
Question
Q: is it okay to use citrate plasma as samples in Human PDGFB Picokine® ELISA Kit (Catalog # EK0484)?
J. Taylor
Verified customer
Asked: 2017-07-01
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of PDGFB causing disruption of its protein structure. PDGFB may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the PDGFB ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-07-01
Question
Q: if the enzyme conjugated PDGFB antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the PDGFB antigen?
Verified Customer
Verified customer
Asked: 2016-12-18
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-12-18
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
P. Harris
Verified customer
Asked: 2016-06-09
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-06-09
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-04-14
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-04-14
