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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human PDGF-AB ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human PDGF-AB ELISA Kit PicoKine™ (96 Tests). Quantitate Human PDGFB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PDGF-AB ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0484)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: (S87-T211) + (S82-T190)
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0484 is reactive to PDGFB in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0484 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PDGFB
The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22. The standard product used in this kit is recombinant human PDGF-AB with the molecular mass of 27KDa.
|EK0484-CAP||Anti-Human PDGFB Pre-coated 96-well strip microplate||1|
|EK0484-ST||Human PDGFB Standard||2 vials, 10 ng/tube|
|EK0484-DA||Human PDGFB Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human PDGF-AB PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PDGF-AB ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of PDGFB in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For PDGFB (Source: Uniprot.Org, NCBI)
Platelet-derived growth factor subunit B
PDGF/VEGF growth factor family
PDGFBB; PDGF-BB PDGFB IBGC5, PDGF-2, PDGF2, SIS, SSV, c-sis platelet derived growth factor subunit B platelet-derived growth factor subunit B|PDGF subunit B|PDGF, B chain|becaplermin|epididymis secretory sperm binding protein|platelet-derived growth factor 2|platelet-derived growth factor B chain|platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)|platelet-derived growth factor, beta polypeptide (oncogene SIS)|proto-oncogene c-Sis*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on PDGFB, check out the PDGFB Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PDGFB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0484 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Anti-inflammatory Effect of Curcumin, Homotaurine, and Vitamin D3 on Human Vitreous in Patients With Diabetic Retinopathy
Functional assessment of autologous platelet-rich plasma (PRP) after long-term storage at −20 °C without any preservation agent
Bone intramedullary reaming grafts the fracture site with CD146+ skeletal progenitors and downmodulates the inflammatory environment
Platelet-derived alpha-granules are associated with inflammation in patients with NK/T-cell lymphoma-associated hemophagocytic syndrome
Takeuchi M, Kamei N, Shinomiya R, Sunagawa T, Suzuki O, Kamoda H, Ohtori S, Ochi M. Neuroreport. 2012 Aug 22;23(12):712-6. Doi: 10.1097/Wnr.0B013E3283567196. Human Platelet-Rich Plasma Promotes Axon Growth In Brain-Spinal Cord Coculture.
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