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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin)|
|Assay Time:||90 Minutes|
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Rat MMP-2 PicoKine™ Quick ELISA Kit
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat MMP-2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Mmp2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat MMP-2 PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0639)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: A30-C662
625 pg/ml - 40,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
FEK0639 is reactive to Mmp2 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin)
FEK0639 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|FEK0639-CAP||Anti-tag Pre-coated 96-well Strip Microplate||1||12 strips of 8 wells|
|FEK0639-ST||Rat MMP-2 Standard||2||40 ng/tube|
|FEK0639-C||Rat Mmp2 Antibody Cocktail||1||6ml|
|AR1106-7||TBS-T Wash Buffer (25x)||1||12ml|
|AR1104||Color Developing Reagent (TMB)||1||10ml|
|PLA-SEA||Adhesive Plate Sealers||2||Piece|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat MMP-2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of MMP-2 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Mmp2 (Source: Uniprot.Org, NCBI)
72 kDa type IV collagenase
peptidase M10A family
72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 184.108.40.206; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase) Mmp2|matrix metallopeptidase 2|72 kDa type IV collagenase|72 kDa gelatinase|MMP-2|gelatinase A|matrix metalloproteinase-2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Mmp2, check out the Mmp2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Mmp2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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