Rat MMP-2 PicoKine® Quick ELISA Kit

MMP-2 ELISA kit, 90-minute one-step protocol

Rat MMP-2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Mmp2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Product Info Summary

SKU: FEK0639
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin)
Assay Time: 90 Minutes

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Product Name

Rat MMP-2 PicoKine® Quick ELISA Kit

See all MMP-2 products

SKU/Catalog Number

FEK0639

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat MMP-2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Mmp2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat MMP-2 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0639)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A30-C662

Sensitivity

<10 pg/ml

Assay Range

625 pg/ml - 40,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0639 is reactive to Mmp2 in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin)

Application Guarantee

FEK0639 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of MMP-2

Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

Kit Components

Catalog numberDescription QuantityVolume
FEK0639-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK0639-STRat MMP-2 Standard 2 40 ng/tube
FEK0639-CRat Mmp2 Antibody Cocktail 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)0625125025005000100002000040000
O.D.0.0290.1150.1730.3100.5370.9761.5552.153

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)20014516236141093454321263
Standard deviation176.09320.641157.0986.35367.981318.31
CV (%)8.8%7.1%4.9%7.9%8.1%6.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MMP-2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1200110939861110129863.584.9%
Sample 245163756453146954375253.725.8%
Sample 323614236972245726546240791396.555.8%
*number of samples for each test n=16.

Gene/Protein Information For Mmp2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P33436

Gene ID

81686

Gene Name

Mmp2

Full Name

72 kDa type IV collagenase

Weight

74.149kDa

Superfamily

peptidase M10A family

Alternative Names

72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase) Mmp2|matrix metallopeptidase 2|72 kDa type IV collagenase|72 kDa gelatinase|MMP-2|gelatinase A|matrix metalloproteinase-2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Mmp2, check out the Mmp2 Infographic

Mmp2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Mmp2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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