Click image to see more details
Product Info Summary
| SKU: | A03683 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CLAUDIN4 CLDN4 Antibody
SKU/Catalog Number
A03683
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CLAUDIN4 CLDN4 Antibody (Catalog # A03683). Tested in ELISA, WB, IF applications. This antibody reacts with Human.
Storage & Handling
CLAUDIN4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CLAUDIN4 CLDN4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03683)
Host
Rabbit
Contents
CLAUDIN4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-CLAUDIN4 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human CLAUDIN4. The immunogen is located within the last 50 amino acids of CLAUDIN4.
Cross-reactivity
At least four isoforms of CLAUDIN4 are known to exist; CLAUDIN4 antibody will detect all four isoforms
Reactive Species
A03683 is reactive to CLDN4 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
22.1 kDa
Background of CLDN4
CLAUDIN4 is an integral membrane protein belonging to the claudin family, a family of cellular adhesion molecules that are components of tight junctions. CLAUDIN4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The CLAUDIN4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems. CLAUDIN4 is frequently overexpressed in ovarian and other epithelial cancers.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03683 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5-2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: CLAUDIN4, A03683 (1 μg/mL), CLAUDIN4, 24-028 (1 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: CLAUDIN4, A03683 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Western Blot Validation in Human Colon Tissue Lysate
Loading: 15 μg of lysates per lane.
Antibodies: CLAUDIN4, A03683 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Western Blot Validation in Human Colon Tissue Lysate
Loading: 15 μg of lysates per lane.
Antibodies: CLAUDIN4, A03683 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Specific Publications For Anti-CLAUDIN4 CLDN4 Antibody (A03683)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CLAUDIN4 CLDN4 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-CLAUDIN4 CLDN4 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
