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Product Info Summary
| SKU: | M01885 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-FSH-beta (Follicle Stimulating Hormone-beta) Monoclonal Antibody
SKU/Catalog Number
M01885
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-FSH-beta (Follicle Stimulating Hormone-beta) Monoclonal Antibody (Catalog # M01885). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-FSH-beta (Follicle Stimulating Hormone-beta) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01885)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM107
Isotype
IgG1, kappa
Immunogen
Full length Purified Human FSH
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M01885 is reactive to FSHB in Human
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
14700 MW
Background of FSH beta
This monoclonal antibody reacts with a protein of 22kDa, identified as beta sub-unit of FSH. It does not cross react with the alpha sub-unit. Follicle stimulating hormone (FSH) is a hormone synthesized and secreted by gonadotrophs in the anterior pituitary gland. In the ovary, FSH stimulates the growth of immature Graafian follicles to maturation. As the follicle grows, it releases inhibin, which deactivates the FSH production. In men, FSH enhances the production of androgen-binding protein by the Sertoli cells of the testis and is critical for spermatogenesis. FSH and LH act synergistically in reproduction. FSH is a useful marker in the classification of pituitary tumors and the study of pituitary disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01885 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml
30 min RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Pituitary stained with FSH-beta Monoclonal Antibody (SPM107).
Protein Target Info & Infographic
Gene/Protein Information For FSHB (Source: Uniprot.org, NCBI)
Gene Name
FSHB
Full Name
Follitropin subunit beta
Weight
14700 MW
Superfamily
glycoprotein hormones subunit beta family
Alternative Names
Follitropin subunit beta;Follicle-stimulating hormone beta subunit;FSH-B;FSH-beta;Follitropin beta chain;FSHB; FSHB HH24 follicle stimulating hormone subunit beta follitropin subunit beta|FSH-B|FSH-beta|follicle stimulating hormone beta subunit|follicle stimulating hormone, beta polypeptide|follitropin, beta chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FSHB, check out the FSHB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FSHB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FSH-beta (Follicle Stimulating Hormone-beta) Monoclonal Antibody (M01885)
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No publications found for M01885
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-FSH-beta (Follicle Stimulating Hormone-beta) Monoclonal Antibody
Question
Does M01885 anti-FSH-beta (Follicle Stimulating Hormone-beta) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-20
Answer
You can see on the product datasheet, M01885 anti-FSH-beta (Follicle Stimulating Hormone-beta) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-20
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pituitary gland using anti-FSH-beta (Follicle Stimulating Hormone-beta) Monoclonal antibody M01885. Let me know if you need anything else.
E. Krishna
Verified customer
Asked: 2017-09-04
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-04
Question
you antibody to test anti-FSH-beta (Follicle Stimulating Hormone-beta) Monoclonal antibody M01885 on human pituitary gland for research purposes, then I may be interested in using anti-FSH-beta (Follicle Stimulating Hormone-beta) Monoclonal antibody M01885 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
O. Brown
Verified customer
Asked: 2017-07-11
Answer
The products we sell, including anti-FSH-beta (Follicle Stimulating Hormone-beta) Monoclonal antibody M01885, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-11
Question
Can you help my question with product M01885, anti-FSH-beta (Follicle Stimulating Hormone-beta) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Taylor
Verified customer
Asked: 2017-03-08
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01885 anti-FSH-beta (Follicle Stimulating Hormone-beta) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-03-08
