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Product Info Summary
| SKU: | A00124-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-IL18 Antibody Picoband®
SKU/Catalog Number
A00124-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL18 Antibody Picoband® catalog # A00124-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL18 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00124-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse IL18 recombinant protein (Position: N36-N177).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00124-1 is reactive to Il18 in Mouse, Rat
Observed Molecular Weight
22 kDa
Calculated molecular weight
22.1 kDa
Background of Il18
Interleukin-18 also known as IL18 is a protein which in humans is encoded by the IL18 gene. The protein encoded by this gene is a proinflammatory cytokine.IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-gamma (IFN-gamma) or type II interferon that plays an important role in activating the macrophages or other cells.The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. The human interleukin 18 gene IL18 maps to 11q22.2-q22.3, closely linked to the DRD2 gene locus and distinct from mapped IDDM loci.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00124-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Mouse, Rat
Flow Cytometry(Fixed), 1-3μg/1x106 cells, Mouse
ELISA, 0.1-0.5μg/ml, -, Rat
Positive Control
WB: rat thymus tissue, rat spleen tissue, rat lung tissue, rat liver tissue, mouse thymus tissue, mouse spleen tissue, mouse RAW2647 whole cell
FCM: ANA-1 cell, mouse spleen tissue
Validation Images & Assay Conditions
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Western blot analysis of IL18 using anti-IL18 antibody (A00124-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse spleen tissue lysates,
Lane 7: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 antigen affinity purified polyclonal antibody (Catalog # A00124-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL18 at approximately 22 kDa. The expected band size for IL18 is at 22 kDa.
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Flow Cytometry analysis of ANA-1 cells using anti-IL18 antibody (A00124-1).
Overlay histogram showing ANA-1 cells stained with A00124-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IL18 Antibody (A00124-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Flow Cytometry analysis of mouse spleen tissues using anti-IL18 antibody (A00124-1).
Overlay histogram showing mouse spleen tissues stained with A00124-1 (Blue line). The tissues were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IL18 Antibody (A00124-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Specific Publications For Anti-IL18 Antibody Picoband® (A00124-1)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-IL18 Antibody Picoband®
Question
Is A00124-1 for the detection of pro-IL-18 or mature IL-18?
Verified customer
Asked: 2020-12-06
Answer
The Anti-IL18 Antibody Picoband™ (A00124-1) is for the detection of pro-IL-18.
Boster Scientific Support
Answered: 2020-12-10
Question
We were content with the WB result of your anti-IL18 antibody. However we have seen positive staining in peripheral blood secreted. using this antibody. Is that expected? Could you tell me where is IL18 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
According to literature, peripheral blood does express IL18. Generally IL18 expresses in secreted. Regarding which tissues have IL18 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 8666798, 15326478
Peripheral blood, Pubmed ID: 10653850
Urinary bladder, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-04-06
Question
I am interested in using your anti-IL18 antibody for detection of mechanical stimulus involved in sensory perception of pain studies. Has this antibody been tested with western blotting on lung tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
Thanks for your inquiry. This A00124-1 anti-IL18 antibody is tested on mouse thymus tissue, spleen tissue, lung tissue, liver tissue, testis tissue. It is guaranteed to work for ELISA, Flow Cytometry, WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-03
Question
We have tried in the past anti-IL18 antibody for WB on lower esophagus mucosa in the past. I am using mouse, and We intend to use the antibody for ELISA next. We want examining lower esophagus mucosa as well as urinary bladder in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
I looked at the website and datasheets of our anti-IL18 antibody and it seems that A00124-1 has been validated on mouse in both WB and ELISA. Thus A00124-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-22
Question
We have been able to see staining in mouse urinary bladder. Do you have any suggestions? Is anti-IL18 antibody supposed to stain urinary bladder positively?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
From literature urinary bladder does express IL18. From Uniprot.org, IL18 is expressed in lower esophagus mucosa, liver, urinary bladder, peripheral blood, among other tissues. Regarding which tissues have IL18 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 8666798, 15326478
Peripheral blood, Pubmed ID: 10653850
Urinary bladder, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-06-25
Question
We are currently using anti-IL18 antibody A00124-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
The anti-IL18 antibody (A00124-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-02
