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- Table of Contents
Il18 antibody (RP1017) performs well in ELISA, IHC, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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IHC analysis of IL18 using anti-IL18 antibody (RP1017).
IL18 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL18 Antibody (RP1017) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
IL18 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL18 Antibody (RP1017) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Designing a Western blot for IL18? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the IL18 Western Blot GuideBundle Save
Anti-Interleukin-18 IL18 Antibody Picoband®
Il18 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | ELISA, IHC, WB |
Tested Applications
Il18 antibody was positively detected by WB in:
mouse J774A1 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Il18 antibody was positively detected by IHC in:
mouse spleen tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Interleukin-18 IL18 Antibody Picoband®
View all IL18 Antibodies
View all IL18 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse IL-18 recombinant protein (Position: N36-S192).
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated Molecular Weight
22.1 kDa
Background of Il18
Interleukin-18 also known as IL18 is a protein which in humans is encoded by the IL18 gene. The protein encoded by this gene is a proinflammatory cytokine.IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-gamma (IFN-gamma) or type II interferon that plays an important role in activating the macrophages or other cells.The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. The human interleukin 18 gene IL18 maps to 11q22.2-q22.3, closely linked to the DRD2 gene locus and distinct from mapped IDDM loci.
Cite This Product
Anti-Interleukin-18 IL18 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1017)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Cow
65%
Low homology
Human
64%
Low homology
Sheep
64%
Low homology
Dog
64%
Low homology
Monkey
62%
Low homology
Rabbit
60%
Low homology
Rat
5%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Il18 antibody
Immunohistochemistry (IHC) protocol for Il18 antibody
Publications
Application:
Species:
Betaine eliminates CFA-induced depressive-like behaviour in mice may be through inhibition of microglia and astrocyte activation and polarization
Published: 2023-12-23
Journal: BRAIN RESEARCH BULLETIN
Application: WB
Impact Factor: 3.80
Species: Mouse
PMID: 38145759
Hydroxysafflor yellow A mitigates myocardial fibrosis induced by isoproterenol and angiotensin II
Published: 2022-12-15
Journal: American Journal of Translational Research
Application: WB,IHC
Impact Factor: 3.94
Species: Mouse
PMID: 36628216
Betaine Ameliorates Acute Sever Ulcerative Colitis by Inhibiting Oxidative Stress Induced Inflammatory Pyroptosis
Published: 2022-09-07
Journal: MOLECULAR NUTRITION & FOOD RESEARCH
Application: WB
Impact Factor: 6.58
Species: Mouse
PMID: 36069237
Targeting PERK mediated endoplasmic reticulum stress attenuates neuroinflammation and alleviates lipopolysaccharide-induced depressive-like behavior in male mice
Published: 2022-08-05
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 5.71
Species: Mouse
PMID: 35940075
Fibrinogen-like protein 2 aggravates nonalcoholic steatohepatitis via interaction with TLR4, eliciting inflammation in macrophages and inducing hepatic lipid metabolism disorder
Published: 2020-08-01
Journal: Theranostics
Application: WB
Impact Factor: 8.58
Species: Mouse
PMID: 32863955
Carnosic acid alleviates brain injury through NF‑κB‑regulated inflammation and Caspase‑3‑associated apoptosis in high fat‑induced mouse models
Published: 2019-05-27
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 1.85
Species: Mouse
PMID: 31180544
Role of interleukin 18 in acute lung inflammation induced by gut ischemia reperfusion
Published: 2005-08-07
Journal: WORLD JOURNAL OF GASTROENTEROLOGY
Application: IHC
Impact Factor: 0.00
Species: Mouse
PMID: 16052682
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Product Promise
We promise Anti-Interleukin-18 IL18 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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RP1017
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 30, 2026