Anti-Interleukin-18 IL18 Antibody

Boster Bio Anti-Interleukin-18 IL18 Antibody catalog # RP1017. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse. Cited in 4 publication(s).

Product Info Summary

SKU: RP1017
Size: 100μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-Interleukin-18 IL18 Antibody

See all IL-18/IL-1F4 products

SKU/Catalog Number

RP1017

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Interleukin-18 IL18 Antibody catalog # RP1017. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Interleukin-18 IL18 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1017)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Immunogen

E. coli-derived mouse IL-18 recombinant protein (Position: N36-S192).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1017 is reactive to Il18 in Mouse

Applications

RP1017 is guaranteed for ELISA, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
ELISA , 0.1-0.5μg/ml, Mouse, -
Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For Il18 (Source: Uniprot.Org, NCBI)

Uniprot ID

P70380

Gene ID

16173

Gene Name

Il18

Full Name

Interleukin-18

Weight

22.135kDa

Superfamily

IL-1 family

Alternative Names

Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18 Il18|Ig, Igif, Il-, Il-18|interleukin 18|interleukin-18|IFN-gamma-inducing factor|IL-1 gamma|interferon gamma-inducing factor|interleukin-1 gamma

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il18, check out the Il18 Infographic

Il18 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il18: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

RP1017 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Yang L,Liu Y,Wang Y,Li J,Liu N. Azeliragon ameliorates Alzheimer‘s disease via the Janus tyrosine kinase and signal transducer and activator of transcription signaling pathway. Clinics (Sao Paulo). 2021 Mar 8;76:e2348.doi:10.6061/clinics/2021/e2348.PMID:33681944;PMCID:PMC7920406.
Species: Rat
RP1017 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:NA

Yang Yj, Shen Y, Chen Sh, Ge Xr. World J Gastroenterol. 2005 Aug 7;11(29):4524-9. Role Of Interleukin 18 In Acute Lung Inflammation Induced By Gut Ischemia Reperfusion.

Wang Y, Zhang X, Zhang Y, Xu H, Fang G. J Reprod Immunol. 2011 Dec;92(1-2):45-53. Doi: 10.1016/J.Jri.2011.07.002. Epub 2011 Sep 13. Expression And Localization Of Il-18 In The Hypothalamic-Pituitary-Ovarian Axis Of Non-Pregnant, Pregnant, And Abor...

Si Lf, Zhang Sy, Gao Cs, Chen Sl, Zhao J, Cheng Xc. Asian-Australas J Anim Sci. 2013 Oct;26(10):1399-405. Doi: 10.5713/Ajas.2013.13101. Effects Of Ifn-?? On Il-18 Expression In Pregnant Rats And Pregnancy Outcomes.

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5 Customer Q&As for Anti-Interleukin-18 IL18 Antibody

Question

Can RP1017 detect pro-IL18 ( precursor) or mature IL-18 (active form)?

Verified customer

Asked: 2020-12-07

Answer

The Anti-Interleukin-18 IL18 Antibody (RP1017) anti-mouse IL18 antibody reacts with precursor IL-18.

Boster Scientific Support

Answered: 2020-12-09

Question

We are currently using anti-IL18 antibody RP1017 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

The anti-IL18 antibody (RP1017) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-14

Question

We have seen staining in mouse peripheral blood. What should we do? Is anti-IL18 antibody supposed to stain peripheral blood positively?

Verified Customer

Verified customer

Asked: 2019-11-06

Answer

According to literature peripheral blood does express IL18. According to Uniprot.org, IL18 is expressed in lower esophagus mucosa, liver, urinary bladder, peripheral blood, among other tissues. Regarding which tissues have IL18 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 8666798, 15326478
Peripheral blood, Pubmed ID: 10653850
Urinary bladder, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-11-06

Question

We ordered your anti-IL18 antibody for IHC on lower esophagus mucosa in the past. I am using mouse, and We are going to use the antibody for ELISA next. you antibody examining lower esophagus mucosa as well as peripheral blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2017-07-12

Answer

I took a look at the website and datasheets of our anti-IL18 antibody and I see that RP1017 has been validated on mouse in both IHC and ELISA. Thus RP1017 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-07-12

Question

Our team were satisfied with the WB result of your anti-IL18 antibody. However we have seen positive staining in peripheral blood secreted. using this antibody. Is that expected? Could you tell me where is IL18 supposed to be expressed?

M. Singh

Verified customer

Asked: 2013-03-22

Answer

Based on literature, peripheral blood does express IL18. Generally IL18 expresses in secreted. Regarding which tissues have IL18 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 8666798, 15326478
Peripheral blood, Pubmed ID: 10653850
Urinary bladder, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2013-03-22

Conjugation

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RP1017
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