Product Info Summary
SKU: | A02685-Biotin |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Rat |
Host: | Rabbit |
Application: | ELISA |
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Product info
Product Name
Anti-HE4/Wfdc2 Biotinylated Antibody
SKU/Catalog Number
A02685-Biotin
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HE4/Wfdc2 Biotinylated Antibody catalog # A02685-Biotin. Tested in ELISA applications. This antibody reacts with Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HE4/Wfdc2 Biotinylated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02685-Biotin)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat HE4 recombinant protein (Position: E31-F168).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02685-Biotin is reactive to WFDC2 in Rat
Applications
A02685-Biotin is guaranteed for ELISA Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
12.993kDa
Background of HE4/WFDC2
WAP four-disulfide core domain protein 2, also known as Human Epididymis Protein 4 (HE4), is a protein that in humans is encoded by the WFDC2 gene. This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA Detection (Matched Antibody Pair), 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For WFDC2 (Source: Uniprot.org, NCBI)
Gene Name
WFDC2
Full Name
WAP four-disulfide core domain protein 2
Weight
12.993kDa
Alternative Names
dJ461P17.6; EDDM4; Epididymal secretory protein E4; epididymis-specific, whey-acidic protein type, four-disulfide core; HE4; HE4MGC57529; Major epididymis-specific protein E4; Putative protease inhibitor WAP5; WAP domain containing protein HE4-V4; WAP four-disulfide core domain 2; WAP four-disulfide core domain protein 2; WAP5; WAP5epididymal protein 4; WFDC2 WFDC2 EDDM4, HE4, WAP5, dJ461P17.6 WAP four-disulfide core domain 2 WAP four-disulfide core domain protein 2|WAP domain containing protein HE4-V4|epididymal protein 4|epididymal secretory protein E4|epididymis secretory sperm binding protein|epididymis-specific, whey-acidic protein type, four-disulfide core|human epididymis protein 4|major epididymis-specific protein E4|putative protease inhibitor WAP5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WFDC2, check out the WFDC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WFDC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HE4/Wfdc2 Biotinylated Antibody (A02685-Biotin)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-HE4/Wfdc2 Biotinylated Antibody
Question
I was wanting to use your anti-HE4/Wfdc2 Biotinylated antibody for ELISA for rat colon on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat colon identification?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
As indicated on the product datasheet, A02685-Biotin anti-HE4/Wfdc2 Biotinylated antibody has been validated for ELISA on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat colon in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-07
Question
Will A02685-Biotin anti-HE4/Wfdc2 Biotinylated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-03
Answer
It shows on the product datasheet, A02685-Biotin anti-HE4/Wfdc2 Biotinylated antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-03
Question
Will anti-HE4/Wfdc2 Biotinylated antibody A02685-Biotin work for ELISA with colon?
Verified Customer
Verified customer
Asked: 2017-11-02
Answer
According to the expression profile of colon, WFDC2 is highly expressed in colon. So, it is likely that anti-HE4/Wfdc2 Biotinylated antibody A02685-Biotin will work for ELISA with colon.
Boster Scientific Support
Answered: 2017-11-02
Question
Is this A02685-Biotin anti-HE4/Wfdc2 Biotinylated antibody reactive to the isotypes of WFDC2?
Verified Customer
Verified customer
Asked: 2017-08-29
Answer
The immunogen of A02685-Biotin anti-HE4/Wfdc2 Biotinylated antibody is E. coli-derived rat HE4 recombinant protein (Position: E31-F168). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-08-29
Question
I have attached the WB image, lot number and protocol we used for colon using anti-HE4/Wfdc2 Biotinylated antibody A02685-Biotin. Please let me know if you require anything else.
P. Baker
Verified customer
Asked: 2016-07-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-07-06
Question
I see that the anti-HE4/Wfdc2 Biotinylated antibody A02685-Biotin works with ELISA, what is the protocol used to produce the result images on the product page?
R. Jackson
Verified customer
Asked: 2015-04-06
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-04-06