Anti-HE4/Wfdc2 Biotinylated Antibody

Boster Bio Anti-HE4/Wfdc2 Biotinylated Antibody catalog # A02685-Biotin. Tested in ELISA applications. This antibody reacts with Rat.

Product Info Summary

SKU: A02685-Biotin
Size: 50ug/vial
Reactive Species: Rat
Host: Rabbit
Application: ELISA

Product Name

Anti-HE4/Wfdc2 Biotinylated Antibody

See all HE4/WFDC2 products

SKU/Catalog Number

A02685-Biotin

Size

50ug/vial

Form

Lyophilized

Description

Boster Bio Anti-HE4/Wfdc2 Biotinylated Antibody catalog # A02685-Biotin. Tested in ELISA applications. This antibody reacts with Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HE4/Wfdc2 Biotinylated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02685-Biotin)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived rat HE4 recombinant protein (Position: E31-F168).

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A02685-Biotin is reactive to Wfdc2 in Rat

Applications

A02685-Biotin is guaranteed for ELISA Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

ELISA Detection (Matched Antibody Pair), 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For Wfdc2 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q14508

Gene ID

10406

Gene Name

Wfdc2

Full Name

WAP four-disulfide core domain protein 2

Weight

12.993kDa

Alternative Names

dJ461P17.6; EDDM4; Epididymal secretory protein E4; epididymis-specific, whey-acidic protein type, four-disulfide core; HE4; HE4MGC57529; Major epididymis-specific protein E4; Putative protease inhibitor WAP5; WAP domain containing protein HE4-V4; WAP four-disulfide core domain 2; WAP four-disulfide core domain protein 2; WAP5; WAP5epididymal protein 4; WFDC2 WFDC2 EDDM4, HE4, WAP5, dJ461P17.6 WAP four-disulfide core domain 2 WAP four-disulfide core domain protein 2|WAP domain containing protein HE4-V4|epididymal protein 4|epididymal secretory protein E4|epididymis secretory sperm binding protein|epididymis-specific, whey-acidic protein type, four-disulfide core|human epididymis protein 4|major epididymis-specific protein E4|putative protease inhibitor WAP5

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Wfdc2, check out the Wfdc2 Infographic

Wfdc2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Wfdc2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Anti-HE4/Wfdc2 Biotinylated Antibody

Question

I was wanting to use your anti-HE4/Wfdc2 Biotinylated antibody for ELISA for rat colon on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat colon identification?

Verified Customer

Verified customer

Asked: 2019-11-07

Answer

As indicated on the product datasheet, A02685-Biotin anti-HE4/Wfdc2 Biotinylated antibody has been validated for ELISA on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat colon in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-07

Question

Will A02685-Biotin anti-HE4/Wfdc2 Biotinylated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-11-03

Answer

It shows on the product datasheet, A02685-Biotin anti-HE4/Wfdc2 Biotinylated antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-11-03

Question

Will anti-HE4/Wfdc2 Biotinylated antibody A02685-Biotin work for ELISA with colon?

Verified Customer

Verified customer

Asked: 2017-11-02

Answer

According to the expression profile of colon, WFDC2 is highly expressed in colon. So, it is likely that anti-HE4/Wfdc2 Biotinylated antibody A02685-Biotin will work for ELISA with colon.

Boster Scientific Support

Answered: 2017-11-02

Question

Is this A02685-Biotin anti-HE4/Wfdc2 Biotinylated antibody reactive to the isotypes of WFDC2?

Verified Customer

Verified customer

Asked: 2017-08-29

Answer

The immunogen of A02685-Biotin anti-HE4/Wfdc2 Biotinylated antibody is E. coli-derived rat HE4 recombinant protein (Position: E31-F168). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-08-29

Question

I have attached the WB image, lot number and protocol we used for colon using anti-HE4/Wfdc2 Biotinylated antibody A02685-Biotin. Please let me know if you require anything else.

P. Baker

Verified customer

Asked: 2016-07-06

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-07-06

Question

I see that the anti-HE4/Wfdc2 Biotinylated antibody A02685-Biotin works with ELISA, what is the protocol used to produce the result images on the product page?

R. Jackson

Verified customer

Asked: 2015-04-06

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-04-06

Conjugation

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A02685-Biotin
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