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Product Info Summary
| SKU: | M00089 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-HGF Mouse Monoclonal Antibody [Clone ID: OTI1D2]
SKU/Catalog Number
M00089
Size
100 µl
Description
Boster Bio HGF mouse monoclonal antibody, clone OTI1D2 (formerly 1D2). Catalog# M00089. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HGF Mouse Monoclonal Antibody [Clone ID: OTI1D2] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00089)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1D2
Isotype
IgG2a
Immunogen
Human recombinant protein fragment corresponding to amino acids 200-494 of human HGF (NP_000592) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00089 is reactive to HGF in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
83.134kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00089 is guaranteed for IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-HGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HGF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HGF (1:2000).
Protein Target Info & Infographic
Gene/Protein Information For HGF (Source: Uniprot.org, NCBI)
Gene Name
HGF
Full Name
Hepatocyte growth factor
Weight
83.134kDa
Superfamily
peptidase S1 family
Alternative Names
deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A HGF DFNB39, F-TCFB, HPTA, SF, HGF hepatocyte growth factor hepatocyte growth factor|fibroblast-derived tumor cytotoxic factor|hepatocyte growth factor (hepapoietin A; scatter factor)|hepatopoietin-A|lung fibroblast-derived mitogen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HGF, check out the HGF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HGF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HGF Mouse Monoclonal Antibody [Clone ID: OTI1D2] (M00089)
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-HGF Mouse Monoclonal Antibody [Clone ID: OTI1D2]
Question
Is a blocking peptide available for product anti-HGF/SF Monoclonal antibody (7-2) (M00089)?
B. Parker
Verified customer
Asked: 2020-04-07
Answer
We do provide the blocking peptide for product anti-HGF/SF Monoclonal antibody (7-2) (M00089). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-07
Question
Is this M00089 anti-HGF/SF Monoclonal antibody (7-2) reactive to the isotypes of HGF?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
The immunogen of M00089 anti-HGF/SF Monoclonal antibody (7-2) is Human Hepatocyte Growth Factor/Scatter Factor. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-20
Question
We are interested in to test anti-HGF/SF Monoclonal antibody (7-2) M00089 on mouse placenta for research purposes, then I may be interested in using anti-HGF/SF Monoclonal antibody (7-2) M00089 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The products we sell, including anti-HGF/SF Monoclonal antibody (7-2) M00089, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-04
Question
Would M00089 anti-HGF/SF Monoclonal antibody (7-2) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Li
Verified customer
Asked: 2020-02-03
Answer
It shows on the product datasheet, M00089 anti-HGF/SF Monoclonal antibody (7-2) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-03
Question
We ordered your anti-HGF/SF Monoclonal antibody (7-2) for WB on placenta a few years ago. I am using human, and We intend to use the antibody for IHC next. I am interested in examining placenta as well as leukocyte in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
I looked at the website and datasheets of our anti-HGF/SF Monoclonal antibody (7-2) and it appears that M00089 has been tested on human in both WB and IHC. Thus M00089 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-17
Question
Please see the WB image, lot number and protocol we used for placenta using anti-HGF/SF Monoclonal antibody (7-2) M00089. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-12
Question
We have seen staining in human embryonic fibroblast. Do you have any suggestions? Is anti-HGF/SF Monoclonal antibody (7-2) supposed to stain embryonic fibroblast positively?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
Based on literature embryonic fibroblast does express HGF. Based on Uniprot.org, HGF is expressed in placenta, liver, leukocyte, lung fibroblast, embryonic fibroblast, brain, among other tissues. Regarding which tissues have HGF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Embryonic fibroblast, Pubmed ID: 1831266
Leukocyte, Pubmed ID: 2145836
Liver, Pubmed ID: 2531289
Lung fibroblast, Pubmed ID: 1824873
Placenta, Pubmed ID: 1826653, 2528952
Boster Scientific Support
Answered: 2019-05-29
Question
Do you have a BSA free version of anti-HGF/SF Monoclonal antibody (7-2) M00089 available?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HGF/SF Monoclonal antibody (7-2) M00089 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-21
Question
Does anti-HGF/SF Monoclonal antibody (7-2) M00089 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
According to the expression profile of placenta, HGF is highly expressed in placenta. So, it is likely that anti-HGF/SF Monoclonal antibody (7-2) M00089 will work for WB with placenta.
Boster Scientific Support
Answered: 2017-06-19
Question
Can you help my question with product M00089, anti-HGF/SF Monoclonal antibody (7-2) . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Jha
Verified customer
Asked: 2014-05-21
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00089 anti-HGF/SF Monoclonal antibody (7-2) , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-05-21
Question
I was wanting to use your anti-HGF/SF Monoclonal antibody (7-2) for WB for mouse placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse placenta identification?
J. Li
Verified customer
Asked: 2013-09-05
Answer
It shows on the product datasheet, M00089 anti-HGF/SF Monoclonal antibody (7-2) has been validated for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-09-05
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-HGF/SF Monoclonal antibody (7-2) M00089. Let me know if you need anything else.
K. Moore
Verified customer
Asked: 2013-08-14
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-08-14
Question
I see that the anti-HGF/SF Monoclonal antibody (7-2) M00089 works with WB, what is the protocol used to produce the result images on the product page?
Z. Wu
Verified customer
Asked: 2013-06-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-06-14
Question
We are currently using anti-HGF/SF Monoclonal antibody (7-2) M00089 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on feline tissues as well?
Z. Johnson
Verified customer
Asked: 2013-01-21
Answer
The anti-HGF/SF Monoclonal antibody (7-2) (M00089) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-01-21
