Anti-HGF Mouse Monoclonal Antibody [Clone ID: OTI1D2]

HGF antibody

Boster Bio HGF mouse monoclonal antibody, clone OTI1D2 (formerly 1D2). Catalog# M00089. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: M00089
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: IHC, WB

Product Name

Anti-HGF Mouse Monoclonal Antibody [Clone ID: OTI1D2]

View all HGF Antibodies

SKU/Catalog Number

M00089

Size

100 µl

Description

Boster Bio HGF mouse monoclonal antibody, clone OTI1D2 (formerly 1D2). Catalog# M00089. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HGF Mouse Monoclonal Antibody [Clone ID: OTI1D2] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00089)

Host

Mouse

Contents

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

OTI1D2

Isotype

IgG2a

Immunogen

Human recombinant protein fragment corresponding to amino acids 200-494 of human HGF (NP_000592) produced in E.coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00089 is reactive to HGF in Human, Mouse, Rat

Reconstitution

Calculated molecular weight

83.134kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M00089 is guaranteed for IHC, WB Boster Guarantee

Validation Images & Assay Conditions

Gene/Protein Information For HGF (Source: Uniprot.org, NCBI)

Gene Name

HGF

Full Name

Hepatocyte growth factor

Weight

83.134kDa

Superfamily

peptidase S1 family

Alternative Names

deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A HGF DFNB39, F-TCFB, HPTA, SF, HGF hepatocyte growth factor hepatocyte growth factor|fibroblast-derived tumor cytotoxic factor|hepatocyte growth factor (hepapoietin A; scatter factor)|hepatopoietin-A|lung fibroblast-derived mitogen

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HGF, check out the HGF Infographic

HGF infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HGF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00089 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Corticosterone Mediates the Inhibitory Effect of Restraint Stress on the Migration of Mesenchymal Stem Cell to Carbon Tetrachloride-Induced Fibrotic Liver by Downregulating CXCR4/7 Expression

Therapeutic role of human hepatocyte growth factor (HGF) in treating hair loss

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14 Customer Q&As for Anti-HGF Mouse Monoclonal Antibody [Clone ID: OTI1D2]

Question

Is a blocking peptide available for product anti-HGF/SF Monoclonal antibody (7-2) (M00089)?

B. Parker

Verified customer

Asked: 2020-04-07

Answer

We do provide the blocking peptide for product anti-HGF/SF Monoclonal antibody (7-2) (M00089). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-07

Question

Is this M00089 anti-HGF/SF Monoclonal antibody (7-2) reactive to the isotypes of HGF?

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

The immunogen of M00089 anti-HGF/SF Monoclonal antibody (7-2) is Human Hepatocyte Growth Factor/Scatter Factor. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-20

Question

We are interested in to test anti-HGF/SF Monoclonal antibody (7-2) M00089 on mouse placenta for research purposes, then I may be interested in using anti-HGF/SF Monoclonal antibody (7-2) M00089 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-04

Answer

The products we sell, including anti-HGF/SF Monoclonal antibody (7-2) M00089, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-04

Question

Would M00089 anti-HGF/SF Monoclonal antibody (7-2) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

K. Li

Verified customer

Asked: 2020-02-03

Answer

It shows on the product datasheet, M00089 anti-HGF/SF Monoclonal antibody (7-2) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-03

Question

We ordered your anti-HGF/SF Monoclonal antibody (7-2) for WB on placenta a few years ago. I am using human, and We intend to use the antibody for IHC next. I am interested in examining placenta as well as leukocyte in our next experiment. Could give a recommendation on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2020-01-17

Answer

I looked at the website and datasheets of our anti-HGF/SF Monoclonal antibody (7-2) and it appears that M00089 has been tested on human in both WB and IHC. Thus M00089 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-01-17

Question

Please see the WB image, lot number and protocol we used for placenta using anti-HGF/SF Monoclonal antibody (7-2) M00089. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-12

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-12

Question

We have seen staining in human embryonic fibroblast. Do you have any suggestions? Is anti-HGF/SF Monoclonal antibody (7-2) supposed to stain embryonic fibroblast positively?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

Based on literature embryonic fibroblast does express HGF. Based on Uniprot.org, HGF is expressed in placenta, liver, leukocyte, lung fibroblast, embryonic fibroblast, brain, among other tissues. Regarding which tissues have HGF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Embryonic fibroblast, Pubmed ID: 1831266
Leukocyte, Pubmed ID: 2145836
Liver, Pubmed ID: 2531289
Lung fibroblast, Pubmed ID: 1824873
Placenta, Pubmed ID: 1826653, 2528952

Boster Scientific Support

Answered: 2019-05-29

Question

Do you have a BSA free version of anti-HGF/SF Monoclonal antibody (7-2) M00089 available?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HGF/SF Monoclonal antibody (7-2) M00089 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-05-21

Question

Does anti-HGF/SF Monoclonal antibody (7-2) M00089 work for WB with placenta?

Verified Customer

Verified customer

Asked: 2017-06-19

Answer

According to the expression profile of placenta, HGF is highly expressed in placenta. So, it is likely that anti-HGF/SF Monoclonal antibody (7-2) M00089 will work for WB with placenta.

Boster Scientific Support

Answered: 2017-06-19

Question

Can you help my question with product M00089, anti-HGF/SF Monoclonal antibody (7-2) . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

M. Jha

Verified customer

Asked: 2014-05-21

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00089 anti-HGF/SF Monoclonal antibody (7-2) , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-05-21

Question

I was wanting to use your anti-HGF/SF Monoclonal antibody (7-2) for WB for mouse placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse placenta identification?

J. Li

Verified customer

Asked: 2013-09-05

Answer

It shows on the product datasheet, M00089 anti-HGF/SF Monoclonal antibody (7-2) has been validated for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-09-05

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-HGF/SF Monoclonal antibody (7-2) M00089. Let me know if you need anything else.

K. Moore

Verified customer

Asked: 2013-08-14

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-08-14

Question

I see that the anti-HGF/SF Monoclonal antibody (7-2) M00089 works with WB, what is the protocol used to produce the result images on the product page?

Z. Wu

Verified customer

Asked: 2013-06-14

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-06-14

Question

We are currently using anti-HGF/SF Monoclonal antibody (7-2) M00089 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on feline tissues as well?

Z. Johnson

Verified customer

Asked: 2013-01-21

Answer

The anti-HGF/SF Monoclonal antibody (7-2) (M00089) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-01-21

$523
Backordered.

Lead time for this item is typically 2 weeks

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M00089
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$523.00

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