Human HGF/Hepatocyte growth factor ELISA Kit PicoKine®

Product Name

Human HGF/Hepatocyte growth factor ELISA Kit PicoKine®

SKU/Catalog Number

EK0369

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

HGF→MET receptor tyrosine kinase signaling engaging PI3K/AKT and MAPK pathways to drive proliferation, motility, morphogenesis, and tissue repair (research). Assay context: sandwich ELISA for human serum/plasma (EDTA, citrate) and cell culture supernatants. Often analyzed with matrix enzymes like MMP-2 to link invasive potential with motogenic cues; may be contrasted with TGF-β axis markers in EMT contexts (putative).

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human HGF/Hepatocyte growth factor ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0369)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: Q32-R494 (alpha) & V495-S728 (beta)

Sensitivity

<5 pg/ml

Assay Range

125 pg/ml - 8,000 pg/ml

Standard Dilution Instructions

See datasheet of EK0369 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0369 is reactive to HGF in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (EDTA, citrate).

Application Guarantee

EK0369 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of HGF

Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.

Kit Components

Catalog Number	Description	Quantity
EK0369-CAP	Anti-Human HGF Pre-coated 96-well strip microplate	1
EK0369-ST	Human HGF Standard	2 vials, 10 ng/tube
EK0369-DA	Human HGF Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.024	0.082	0.142	0.273	0.523	1.03	1.741	2.466

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect HGF, For serum, dilution ratio is 1:1 and concentration is 799-3642 pg/ml.
For plasma, dilution ratio is 1:1 and concentration is 375-1946 pg/ml..

Some articles we found to cite concentrations of HGF in samples: 25697336 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human HGF/Hepatocyte growth factor ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

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