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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (EDTA, citrate).|
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Human HGF/Hepatocyte growth factor ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human HGF/Hepatocyte growth factor ELISA Kit PicoKine™ (96 Tests). Quantitate Human HGF in cell culture supernatants, serum and plasma (EDTA, citrate). Sensitivity: 5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human HGF/Hepatocyte growth factor ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0369)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Q32-R494 (alpha) & V495-S728 (beta)
125 pg/ml - 8,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0369 is reactive to HGF in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (EDTA, citrate).
EK0369 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0369-CAP||Anti-Human HGF Pre-coated 96-well strip microplate||1|
|EK0369-ST||Human HGF Standard||2 vials, 10 ng/tube|
|EK0369-DA||Human HGF Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human HGF PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human HGF/Hepatocyte growth factor ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of HGF in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For HGF (Source: Uniprot.Org, NCBI)
Hepatocyte growth factor
peptidase S1 family
deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 188.8.131.52; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A HGF DFNB39, F-TCFB, HPTA, SF, HGF hepatocyte growth factor hepatocyte growth factor|fibroblast-derived tumor cytotoxic factor|hepatocyte growth factor (hepapoietin A; scatter factor)|hepatopoietin-A|lung fibroblast-derived mitogen*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on HGF, check out the HGF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for HGF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0369 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Casciaro F,Zia S,Forcato M,Zavatti M,Beretti F,Bertucci E,Zattoni A,Reschiglian P,Alviano F,Bonsi L,Follo MY,Demaria M,Roda B,Maraldi T.Unravelling Heterogeneity of Amplified Human Amniotic Fluid Stem Cells Sub-Populations.Cells.2021 Jan 15;10(1):158.doi:
EK0369 usage in article: APP:ELISA, SAMPLE:HAFSCS, DILUTION:NA
Serial changes of cytokines and growth factors in peripheral circulation after right lobe donor hepatectomy
Substrate-mediated delivery of gene complex nanoparticles via polydopamine coating for enhancing competitiveness of endothelial cells
Significance of epithelial growth factor in the epithelial-mesenchymal transition of human gallbladder cancer cells
Utility of Hepatocyte Growth Factor as a Biomarker for Early Diagnosis of Pulmonary Artery Hypertension
Human biliary tree stem/progenitor cells immunomodulation: Role of hepatocyte growth factor.
Assessment of the involvement of the macrophage migration inhibitory factor%u2013glucocorticoid regulatory dyad in the expression of matrix metalloproteinase%u20102 during %u2026
Role of Hepatocyte Growth Factor in the Immunomodulation Potential of Amniotic Fluid Stem Cells
Li J, Hu SB, Wang LY, Zhang X, Zhou X, Yang B, Li JH, Xiong J, Liu N, Li Y, Wu YZ, Zheng QC. Oncogene. 2017 Nov 30;36(48):6725-6737. doi: 10.1038/onc.2017.272. Epub 2017 Aug 7. Autophagy-dependent generation of Axin2+ cancer stem-like cells promot...
Guo Zy, Sun X, Xu Xl, Zhao Q, Peng J, Wang Y. Neural Regen Res. 2015 Apr;10(4):651-8. Doi: 10.4103/1673-5374.155442. Human Umbilical Cord Mesenchymal Stem Cells Promote Peripheral Nerve Repair Via Paracrine Mechanisms.
Secchiero P, Corallini F, Zavan B, Tripodo C, Vindigni V, Zauli G. Invest New Drugs. 2012 Apr;30(2):803-7. Doi: 10.1007/S10637-010-9534-Z. Epub 2010 Sep 9. Mesenchymal Stem Cells Display Hepato-Protective Activity In Lymphoma Bearing Xenografts.
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