Human HGF/Hepatocyte growth factor ELISA Kit PicoKine®

HGF ELISA kit for Human

Human HGF/Hepatocyte growth factor ELISA Kit PicoKine™ (96 Tests). Quantitate Human HGF in cell culture supernatants, serum and plasma (EDTA, citrate). Sensitivity: 5pg/ml. Cited in 22 publication(s).

Product Info Summary

SKU: EK0369
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (EDTA, citrate).

Product Name

Human HGF/Hepatocyte growth factor ELISA Kit PicoKine®

View all HGF ELISA kits

SKU/Catalog Number

EK0369

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human HGF/Hepatocyte growth factor ELISA Kit PicoKine™ (96 Tests). Quantitate Human HGF in cell culture supernatants, serum and plasma (EDTA, citrate). Sensitivity: 5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human HGF/Hepatocyte growth factor ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0369)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q32-R494 (alpha) & V495-S728 (beta)

Sensitivity

<5 pg/ml

Assay Range

125 pg/ml - 8,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0369 is reactive to HGF in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (EDTA, citrate).

Application Guarantee

EK0369 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of HGF

Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.

Kit Components

Catalog Number Description Quantity
EK0369-CAP Anti-Human HGF Pre-coated 96-well strip microplate 1
EK0369-ST Human HGF Standard 2 vials, 10 ng/tube
EK0369-DA Human HGF Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)01252505001000200040008000
O.D.0.0140.0810.1160.2170.3570.6311.2612.033

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human HGF/Hepatocyte growth factor ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)1691246418316813644239
Standard deviation9.887.22267.719.91118.66313.68
CV (%)5.8%7%6.4%5.9%8.7%7.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of HGF in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11691591651521616.413.9%
Sample 21246110311321240118063.615.3%
Sample 341833905430836574013252.156.2%
*number of samples for each test n=16.

Gene/Protein Information For HGF (Source: Uniprot.Org, NCBI)

Uniprot ID

P14210

Gene ID

3082

Gene Name

HGF

Full Name

Hepatocyte growth factor

Weight

83.134kDa

Superfamily

peptidase S1 family

Alternative Names

deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A HGF DFNB39, F-TCFB, HPTA, SF, HGF hepatocyte growth factor hepatocyte growth factor|fibroblast-derived tumor cytotoxic factor|hepatocyte growth factor (hepapoietin A; scatter factor)|hepatopoietin-A|lung fibroblast-derived mitogen

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on HGF, check out the HGF Infographic

HGF infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for HGF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0369 has been cited in 22 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Role of Hepatocyte Growth Factor in the Immunomodulation Potential of Amniotic Fluid Stem Cells

Unravelling Heterogeneity of Amplified Human Amniotic Fluid Stem Cells Sub-Populations

Twist1 Contributes to the Maintenance of Some Biological Properties of Dermal Papilla Cells in vitro by Forming a Complex With Tcf4 and β-Catenin

Human biliary tree stem/progenitor cells immunomodulation: Role of hepatocyte growth factor

Cucurbit[8]uril Supramolecular Assembly for Positively Charged Ultrathin Films as Nanocontainers

Substrate-mediated delivery of gene complex nanoparticles via polydopamine coating for enhancing competitiveness of endothelial cells

Utility of Hepatocyte Growth Factor as a Biomarker for Early Diagnosis of Pulmonary Artery Hypertension

Serial changes of cytokines and growth factors in peripheral circulation after right lobe donor hepatectomy

Exosomes derived from adipose tissue, bone marrow, and umbilical cord blood for cardioprotection after myocardial infarction

The (PrS/HGF‐pDNA) multilayer films for gene‐eluting stent coating: Gene‐protecting, anticoagulation, antibacterial properties, and in vivo antirestenosis evaluation

Surface-mediated functional gene delivery: An effective strategy for enhancing competitiveness of endothelial cells over smooth muscle cells
Species: Human

Casciaro F,Zia S,Forcato M,Zavatti M,Beretti F,Bertucci E,Zattoni A,Reschiglian P,Alviano F,Bonsi L,Follo MY,Demaria M,Roda B,Maraldi T.Unravelling Heterogeneity of Amplified Human Amniotic Fluid Stem Cells Sub-Populations.Cells.2021 Jan 15;10(1):158.doi:
Species: Human
EK0369 usage in article: APP:ELISA, SAMPLE:HAFSCS, DILUTION:NA

Serial changes of cytokines and growth factors in peripheral circulation after right lobe donor hepatectomy

Substrate-mediated delivery of gene complex nanoparticles via polydopamine coating for enhancing competitiveness of endothelial cells

Significance of epithelial growth factor in the epithelial-mesenchymal transition of human gallbladder cancer cells

Utility of Hepatocyte Growth Factor as a Biomarker for Early Diagnosis of Pulmonary Artery Hypertension

Human biliary tree stem/progenitor cells immunomodulation: Role of hepatocyte growth factor.

Assessment of the involvement of the macrophage migration inhibitory factor%u2013glucocorticoid regulatory dyad in the expression of matrix metalloproteinase%u20102 during %u2026

Role of Hepatocyte Growth Factor in the Immunomodulation Potential of Amniotic Fluid Stem Cells

Li J, Hu SB, Wang LY, Zhang X, Zhou X, Yang B, Li JH, Xiong J, Liu N, Li Y, Wu YZ, Zheng QC. Oncogene. 2017 Nov 30;36(48):6725-6737. doi: 10.1038/onc.2017.272. Epub 2017 Aug 7. Autophagy-dependent generation of Axin2+ cancer stem-like cells promot...

Guo Zy, Sun X, Xu Xl, Zhao Q, Peng J, Wang Y. Neural Regen Res. 2015 Apr;10(4):651-8. Doi: 10.4103/1673-5374.155442. Human Umbilical Cord Mesenchymal Stem Cells Promote Peripheral Nerve Repair Via Paracrine Mechanisms.

Secchiero P, Corallini F, Zavan B, Tripodo C, Vindigni V, Zauli G. Invest New Drugs. 2012 Apr;30(2):803-7. Doi: 10.1007/S10637-010-9534-Z. Epub 2010 Sep 9. Mesenchymal Stem Cells Display Hepato-Protective Activity In Lymphoma Bearing Xenografts.

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21 Customer Q&As for Human HGF/Hepatocyte growth factor ELISA Kit PicoKine®

Question

What is the lower limit of quantification (LLOQ) and measuring absorbance (wavelength) of EK0369?

Verified customer

Asked: 2022-05-06

Answer

For the Human HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK0369), the lower limit of quantification (LLOQ) is 125pg/ml and measuring absorbance (wavelength) is 450nm.

Boster Scientific Support

Answered: 2022-05-07

Question

What tissue types have you tested with EK0369?

Verified customer

Asked: 2022-05-06

Answer

Our lab hasn't tested any tissue with the Human HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK0369). Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend to run a pilot test as described below to confirm the affect of endogeous biotin first; Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, the customer can work on the tissue sample by using the kit.

Boster Scientific Support

Answered: 2022-05-07

Question

What plasma/serum concentration did you see in your samples when preparing EK0369?

Verified customer

Asked: 2022-05-06

Answer

The plasma/serum concentration observed is 300-3300pg/ml.

Boster Scientific Support

Answered: 2022-05-07

Question

Is there a recommended dilution factor for plasma/serum samples using EK0369?

Verified customer

Asked: 2022-05-06

Answer

The recommended dilution factor for the Human HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK0369) is 1:1.

Boster Scientific Support

Answered: 2022-05-07

Question

What is the Host Species for the capture and detection antibodies of EK0369?

Verified customer

Asked: 2022-05-06

Answer

For the Human HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK0369), the Host Species for the capture antibody is mouse while the Host Species for the detection antibody is goat.

Boster Scientific Support

Answered: 2022-05-07

Question

What is the Isotype for the capture and detection antibodies of EK0369?

Verified customer

Asked: 2022-05-06

Answer

For the Human HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK0369), the capture antibody Isotype is IgG1 while the detection antibody Isotype is IgG.

Boster Scientific Support

Answered: 2022-05-07

Question

What is the clonality for the capture and detection antibodies of EK0369?

Verified customer

Asked: 2022-05-06

Answer

For the Human HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK0369), the capture antibody clonality is monoclonal while the detection antibody clonality is polyclonal.

Boster Scientific Support

Answered: 2022-05-07

Question

What is the peptide sequence or general region of the protein used for EK0369?

Verified customer

Asked: 2022-05-06

Answer

The peptide sequence or general region of the protein used for EK0369 is Q32-R494 (alpha) & V495-S728 (beta).

Boster Scientific Support

Answered: 2022-05-07

Question

What expression system was used in EK0369 if the immunogen is a recombinant protein?

Verified customer

Asked: 2022-05-06

Answer

NS0 was used for the standard of the Human HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK0369).

Boster Scientific Support

Answered: 2022-05-07

Question

What is the sensitivity of EK0369?

Verified customer

Asked: 2022-05-06

Answer

The Human HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK0369) sensitivity is <5 pg/ml.

Boster Scientific Support

Answered: 2022-05-07

Question

Has the cross-reactivity of EK0369 been tested?

Verified customer

Asked: 2022-05-06

Answer

Our lab tested the recombinant proteins listed below using the Human HGF/Hepatocyte Growth Factor ELISA Kit PicoKine® (EK0369) and no obvious cross-reactivity was observed. Recombinant human: EGF, G-CSF, GM-CSF, HGF R, M-CSF, Met, PDGF-AA, PDGF-AB, PDGF-BB, VEGF

Boster Scientific Support

Answered: 2022-05-07

Question

Q: if the enzyme conjugated HGF antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the HGF antigen?

D. Sunder

Verified customer

Asked: 2020-04-06

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-04-06

Question

Q: how do I analyze ELISA data? I have performed HGF level in plasma.

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-02-14

Question

Does EK0369 cross react with mouse?

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

EK0369 does not cross react with mouse.

Boster Scientific Support

Answered: 2019-09-26

Question

Does EK0369 detect the active form of HGF?

Verified Customer

Verified customer

Asked: 2019-06-13

Answer

EK0369 is able to detect the active form of HGF.

Boster Scientific Support

Answered: 2019-06-13

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

K. Kaur

Verified customer

Asked: 2018-09-17

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-09-17

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Y. Zhao

Verified customer

Asked: 2018-09-03

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-09-03

Question

Q: can I use heparin plasma as samples in Human HGF Picokine® ELISA Kit (Catalog # EK0369)?

C. Jha

Verified customer

Asked: 2018-06-21

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of HGF causing degradation of its protein structure. HGF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the HGF ELISA, treating samples with a number of anticoagulants and decided that EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-06-21

Question

I hope that you week is going well! I have a customer interested in this product but has a few questions. Could you assist? Could you let me know if: 1) the antibodies are monoclonal or polyclonal? 2) Whether cross species reactivity to mouse has been checked? 3) Does this kit work on tissue lysate?

Verified Customer

Verified customer

Asked: 2017-07-21

Answer

The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. EK0369 doesn't cross react with mouse.

Boster Scientific Support

Answered: 2017-07-21

Question

Q: can you recommend the dilution ratio of serum samples for detection of HGF in Human plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

A. Campbell

Verified customer

Asked: 2014-11-29

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the HGF ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2014-11-29

Question

Is this kit able to detect the active form of HGF?

Verified Customer

Verified customer

Asked: 2013-05-10

Answer

EK0369 is able to detect the active form of HGF.

Boster Scientific Support

Answered: 2013-05-10

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