Human HGF ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human HGF. 96wells/kit, with removable strips. Cited in 7 publication(s).
Human HGF ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, serum and plasma(EDTA, citrate).|
|Sample Volume:||100μl per well|
|Product Name||Human HGF ELISA Kit PicoKine™
See all HGF primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human HGF with <5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. HGF tissue specificity:|
|Cite This Product||Human HGF ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0369)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum and plasma(EDTA, citrate).|
|Immunogen||Expression system for standard: sf21; Immunogen sequence: Q32-R494(alpha)&V495-S728(beta)|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Protein Target Info (Source: Uniprot.org)
|Protein Name||Hepatocyte growth factor|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. .|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Kit Components And Assay QC Data Details Of Human HGF ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0369-CAP||96-well plate precoated with anti-Human HGF antibody||1|
|EK0369-ST||lyophilized recombinant Human HGF standard||10ng/tube|
|EK0369-DA||biotinylated anti-Human HGF antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0369 Human HGF ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human HGF ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human HGF ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 7
|Secchiero P, Corallini F, Zavan B, Tripodo C, Vindigni V, Zauli G. Invest New Drugs. 2012 Apr;30(2):803-7. Doi: 10.1007/S10637-010-9534-Z. Epub 2010 Sep 9. Mesenchymal Stem Cells Display Hepato-Protective Activity In Lymphoma Bearing Xenografts.||PubMed ID 20827501|
|Guo Zy, Sun X, Xu Xl, Zhao Q, Peng J, Wang Y. Neural Regen Res. 2015 Apr;10(4):651-8. Doi: 10.4103/1673-5374.155442. Human Umbilical Cord Mesenchymal Stem Cells Promote Peripheral Nerve Repair Via Paracrine Mechanisms.||PubMed ID 26170829|
|Li J, Hu SB, Wang LY, Zhang X, Zhou X, Yang B, Li JH, Xiong J, Liu N, Li Y, Wu YZ, Zheng QC. Oncogene. 2017 Nov 30;36(48):6725-6737. doi: 10.1038/onc.2017.272. Epub 2017 Aug 7. Autophagy-dependent generation of Axin2+ cancer stem-like cells promot...||PubMed ID 28783177|
|Role of Hepatocyte Growth Factor in the Immunomodulation Potential of Amniotic Fluid Stem Cells||PubMed ID 25873747|
|Assessment of the involvement of the macrophage migration inhibitory factor%u2013glucocorticoid regulatory dyad in the expression of matrix metalloproteinase%u20102 during %u2026||PubMed ID 28776753|
|Human biliary tree stem/progenitor cells immunomodulation: Role of hepatocyte growth factor.||PubMed ID 27381820|
|Utility of Hepatocyte Growth Factor as a Biomarker for Early Diagnosis of Pulmonary Artery Hypertension||PubMed ID 27342109|
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Note: sample size takes longer to process, average 5-7 days.
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