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Product Info Summary
| SKU: | M06510 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Primate |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IP, IHC-F, ICC |
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Product info
Product Name
Anti-Hu CD170 Purified SIGLEC5 Monoclonal Antibody
SKU/Catalog Number
M06510
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD170 Purified SIGLEC5 Monoclonal Antibody (Catalog# M06510). Tested in Flow Cytometry, IP, IHC-F, ICC, ELISA application(s). This antibody reacts with Primate, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD170 Purified SIGLEC5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06510)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
1A5
Isotype
Mouse IgG1
Immunogen
Fusion protein composed of human CD170 extracellular domain and Fc region of human IgG1. The mouse monoclonal antibody 1A5 recognizes an extracellular epitope of CD170 (Siglec-5, sialic acid binding Ig-like lectin 5), a transmembrane glycoprotein expressed strongly by neutrophils, macrophages activated during infections, monocytes, and dendritic cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M06510 is reactive to SIGLEC5 in Human, Primate
Applications
M06510 is guaranteed for ELISA, Flow Cytometry, IP, IHC-F, ICC Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
60.715kDa
Background of SIGLEC5
CD170, also known as Siglec 5 (sialic acid binding Ig-like lectin 5) is a type 1 transmembrane glycoprotein containing two cytoplasmic immunoreceptor tyrosine inhibitory motifs (ITIMs). CD170 forms homodimers and functions as an inhibitory receptor able to downregulate cell activation. It binds to alpha2,3- and alpha2,6-linked sialic acid ligands, e.g. on glycophorin A (CD235a). Aberrant expression of CD170 by CD34+ progenitor cells can be observed in case of acute myeloid leukemias.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Immunohistochemistry: 5-10 μg/ml.
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry validation of SIGLEC5 using Anti-Hu CD170 Purified SIGLEC5 Monoclonal Antibody (M06510).
Flow cytometry (surface staining) of human peripheral blood cells using anti-CD170 (1A5) purifiedProtein Target Info & Infographic
Gene/Protein Information For SIGLEC5 (Source: Uniprot.org, NCBI)
Gene Name
SIGLEC5
Full Name
Sialic acid-binding Ig-like lectin 5
Weight
60.715kDa
Superfamily
immunoglobulin superfamily
Alternative Names
Siglec-5/Siglec-14 SIGLEC5 CD170, CD33L2, OB-BP2, OBBP2, SIGLEC-5 sialic acid binding Ig like lectin 5 sialic acid-binding Ig-like lectin 5|CD33 antigen-like 2|OB-binding protein 2|obesity-binding protein 2|sialic acid-binding immunoglobulin-like lectin 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIGLEC5, check out the SIGLEC5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIGLEC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD170 Purified SIGLEC5 Monoclonal Antibody (M06510)
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No publications found for M06510
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