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Product Info Summary
| SKU: | M01241-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Primate |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Hu CD62P Purified SELP Monoclonal Antibody
SKU/Catalog Number
M01241-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD62P Purified SELP Monoclonal Antibody (Catalog# M01241-1). Tested in Flow Cytometry, IP, WB, ICC, IHC-F application(s). This antibody reacts with Human, Primate.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD62P Purified SELP Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01241-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
AK4
Isotype
Mouse IgG1 kappa
Immunogen
Human platelets. The antibody AK4 recognizes an extracellular epitope of CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.
Reactive Species
M01241-1 is reactive to SELP in Human, Primate
Observed Molecular Weight
42 kDa
Calculated molecular weight
90.8 kDa
Background of SELP
CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01241-1 is guaranteed for Flow Cytometry, IP, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1.5 μg/ml.
Validation Images & Assay Conditions
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Separation of human thrombocytes (red-filled) from CD62P negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62P (AK4) purified antibody (concentration in sample 1 µg/ml) GAM APC.
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Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD62P (AK4) purified antibody (concentration in sample 1 µg/ml) GAM APC.
Specific Publications For Anti-Hu CD62P Purified SELP Monoclonal Antibody (M01241-1)
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