Human ADAMTS13 ELISA Kit PicoKine®

ADAMTS13 ELISA kit for Human

Human ADAMTS13 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ADAMTS13 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 20pg/ml.

Product Info Summary

SKU: EK0927
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Human ADAMTS13 ELISA Kit PicoKine®

View all ADAMTS13 ELISA kits

SKU/Catalog Number

EK0927

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human ADAMTS13 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ADAMTS13 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 20pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human ADAMTS13 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0927)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q34-W688

Sensitivity

<20 pg/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0927 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0927 is reactive to ADAMTS13 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0927 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of ADAMTS13

ADAMTS13 is a zinc-containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal-containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. And it is confirmed that the ADAMTS13 gene encodes the von Willebrand factor-cleaving protease (VWFCP). ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity.

Kit Components

Catalog Number Description Quantity
EK0927-CAP Anti-Human ADAMTS13 Pre-coated 96-well strip microplate 1
EK0927-ST Human ADAMTS13 Standard 2 vials, 50 ng/tube
EK0927-DA Human ADAMTS13 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)00.781.563.126.2512.52550
O.D.0.0790.2080.2950.4800.7331.2281.6492.001

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect ADAMTS13, Dilution ratio of 1:100, concentration in serum and plasma is around 500 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human ADAMTS13 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)1313227236208161322661720301
Standard deviation104.62289.441164.9104.43291.141197.75
CV (%)7.5%4%5.6%7.9%4.4%5.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of ADAMTS13 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11395132313921567141990.036.3%
Sample 272368286781280647849391.894.9%
Sample 320816202912243322964216261104.515.1%
*number of samples for each test n=16.

Gene/Protein Information For ADAMTS13 (Source: Uniprot.Org, NCBI)

Gene Name

ADAMTS13

Full Name

A disintegrin and metalloproteinase with thrombospondin motifs 13

Weight

153.604kDa

Alternative Names

A disintegrin and metalloproteinase with thrombospondin motifs 13; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 13; ADAM metallopeptidase with thrombospondin type 1 motif, 13; ADAMTS13; ADAM-TS13; ADAMTS-13; DKFZp434C2322; EC 3.4.24.14; EC 3.4.24.82; EC 3.4.24.87; FLJ42993; MGC118899; MGC118900; TTP; TTPADAM-TS 13; vWF-cleaving protease; vWF-CP; vWF-CPC9orf8; VWFCPvon Willebrand factor-cleaving protease ADAMTS13 ADAM-TS13, ADAMTS-13, C9orf8, VWFCP, vWF-CP ADAM metallopeptidase with thrombospondin type 1 motif 13 A disintegrin and metalloproteinase with thrombospondin motifs 13|a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13|vWF-cleaving protease|von Willebrand factor-cleaving protease

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ADAMTS13, check out the ADAMTS13 Infographic

ADAMTS13 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ADAMTS13: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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14 Customer Q&As for Human ADAMTS13 ELISA Kit PicoKine®

Question

For EK0927, would you please advise the following: If it is included in the product and is not listed in the MSDS, please tell me the concentration of the following substances. ・Sulfuric acid ・Sodium azide

Verified customer

Asked: 2022-11-30

Answer

The kit doesn't contain sodium azide and the stop solution contains 2% Sulfuric acid. Please see MSDS. https://www.bosterbio.com/msds?sku=EK0927

Boster Scientific Support

Answered: 2022-11-30

Question

If a recombinant protein such as Standard or coating antigen is included in the kit, please tell me the expression species of the protein and whether viral vectors are use.

Verified customer

Asked: 2022-11-30

Answer

The standard in EK0927 is a recombinant protein. Expression system of the standard is CHO and no viral vector is used.

Boster Scientific Support

Answered: 2022-11-30

Question

Do the kit contents include human biological samples?

Verified customer

Asked: 2022-11-30

Answer

It doesn't contain any human biological samples.

Boster Scientific Support

Answered: 2022-11-30

Question

What s the standard source for EK0927?

Verified customer

Asked: 2021-02-16

Answer

The standard for the Human ADAMTS13 ELISA Kit PicoKine™ (EK0927) is a recombinant protein.

Boster Scientific Support

Answered: 2021-02-22

Question

Is there a minimum dilution prescribed for serum and plasma samples using EK0927?

Verified customer

Asked: 2021-02-16

Answer

For the Human ADAMTS13 ELISA Kit PicoKine™ (EK0927), the suggested dilution factor for serum and plasma samples is 1:5. 1:15 is higher than it is needed.

Boster Scientific Support

Answered: 2021-02-22

Question

Q: Can ADAMTS13 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

V. Wright

Verified customer

Asked: 2019-10-26

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-10-26

Question

What is the expected concentration range of ADAMTS13 in normal human serum and plasma? Also could you provide the recommend dilution ratios?

Verified customer

Asked: 2019-04-22

Answer

The expected concentration range of ADAMTS13 in normal human serum and plasma is 40-800ng/mL. The recommended dilution range is 1:10-1:50.

Boster Scientific Support

Answered: 2019-04-22

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-03-09

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-03-09

Question

Q: how do I analyze ELISA data? I have performed ADAMTS13 level in plasma.

Verified Customer

Verified customer

Asked: 2018-11-14

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-11-14

Question

Q: if the enzyme conjugated ADAMTS13 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the ADAMTS13 antigen?

Verified Customer

Verified customer

Asked: 2017-11-10

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-11-10

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

B. Edwards

Verified customer

Asked: 2017-08-28

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-08-28

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

T. White

Verified customer

Asked: 2016-06-01

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-06-01

Question

Q: What is the optimal O.D. value for ADAMTS13 ELISA kit? I performed your ADAMTS13 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain ADAMTS13 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2016-02-17

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this ADAMTS13 ELISA kit to have sensitivity of 20pg/ml, that means the minimum amount of ADAMTS13 that can be declared/interpreted as positive by the above standard is 20pg/ml.

Boster Scientific Support

Answered: 2016-02-17

Question

Q: can you suggest the dilution ratio of serum samples for detection of ADAMTS13 in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2015-06-29

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the ADAMTS13 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2015-06-29

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