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Product Info Summary
| SKU: | A04169-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB, ELISA (Cap) |
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Product info
Product Name
Anti-ESM1 Antibody Picoband®
SKU/Catalog Number
A04169-1
Size
100 μg/vial
Form
Lyophilized
Description
ESM1 (endocan) is a secreted dermatan-sulfate proteoglycan expressed by vascular endothelium, implicated in angiogenic sprouting and endothelial–leukocyte interactions. Assay context (per provided product description; product page not retrievable via tool at curation time): Human-reactive Picoband antibody validated for Western blot. Often interpreted with vascular/growth and matrix-remodeling panels such as FGF2 and ECM protease–inhibitor pairs like TIMP3 vs MMP9 (putative co-panel).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ESM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04169-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human ESM1 recombinant protein (Position: W20-R184).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04169-1 is reactive to ESM1 in Human
Observed Molecular Weight
25 kDa
Calculated molecular weight
20.1 kDa
Background of ESM1
Endothelial cell-specific molecule 1 is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04169-1 is guaranteed for WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml
Validation Images & Assay Conditions
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Western blot analysis of ESM1 using anti-ESM1 antibody (A04169-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human ESM1 protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESM1 antigen affinity purified polyclonal antibody (Catalog # A04169-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESM1 at approximately 25KD. The expected band size for ESM1 is at 20KD.
Specific Publications For Anti-ESM1 Antibody Picoband® (A04169-1)
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Customer Q&As
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13 Customer Q&As for Anti-ESM1 Antibody Picoband®
Question
Would anti-ESM1 antibody A04169-1 work for ELISA with umbilical vein endothelial cell?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
According to the expression profile of umbilical vein endothelial cell, ESM1 is highly expressed in umbilical vein endothelial cell. So, it is likely that anti-ESM1 antibody A04169-1 will work for ELISA with umbilical vein endothelial cell.
Boster Scientific Support
Answered: 2020-04-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for umbilical vein endothelial cell using anti-ESM1 antibody A04169-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-03
Question
See below the WB image, lot number and protocol we used for umbilical vein endothelial cell using anti-ESM1 antibody A04169-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-22
Question
I see that the anti-ESM1 antibody A04169-1 works with ELISA, what is the protocol used to produce the result images on the product page?
M. Evans
Verified customer
Asked: 2020-01-21
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-21
Question
Would anti-ESM1 antibody A04169-1 work on zebrafish ELISA with umbilical vein endothelial cell?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
Our lab technicians have not tested anti-ESM1 antibody A04169-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-ESM1 antibody A04169-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish umbilical vein endothelial cell in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-30
Question
Our lab want to know about to test anti-ESM1 antibody A04169-1 on human umbilical vein endothelial cell for research purposes, then I may be interested in using anti-ESM1 antibody A04169-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
The products we sell, including anti-ESM1 antibody A04169-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-04
Question
Can you help my question with product A04169-1, anti-ESM1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Thomas
Verified customer
Asked: 2019-05-09
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04169-1 anti-ESM1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-09
Question
Is there a BSA free version of anti-ESM1 antibody A04169-1 available?
Verified Customer
Verified customer
Asked: 2019-05-01
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ESM1 antibody A04169-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-01
Question
We are currently using anti-ESM1 antibody A04169-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
The anti-ESM1 antibody (A04169-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-14
Question
I was wanting to use your anti-ESM1 antibody for ELISA for human umbilical vein endothelial cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human umbilical vein endothelial cell identification?
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
As indicated on the product datasheet, A04169-1 anti-ESM1 antibody has been tested for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human umbilical vein endothelial cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-23
Question
Will A04169-1 anti-ESM1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Miller
Verified customer
Asked: 2018-07-16
Answer
It shows on the product datasheet, A04169-1 anti-ESM1 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-16
Question
Is a blocking peptide available for product anti-ESM1 antibody (A04169-1)?
W. Lewis
Verified customer
Asked: 2016-06-06
Answer
We do provide the blocking peptide for product anti-ESM1 antibody (A04169-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2016-06-06
Question
Is this A04169-1 anti-ESM1 antibody reactive to the isotypes of ESM1?
F. Yang
Verified customer
Asked: 2013-01-30
Answer
The immunogen of A04169-1 anti-ESM1 antibody is E. coli-derived human ESM1 recombinant protein (Position: W20-R184). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-01-30
