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- Table of Contents
IL1B antibody (PA1341) performs well in WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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Anti-IL-1 beta antibody, PA1341, Western blotting
Lane 1: Recombinant Human IL-1 beta Protein 20ng
Lane 2: Recombinant Human IL-1 beta Protein 10ng
Lane 1: Recombinant Human IL-1 beta Protein 20ng
Lane 2: Recombinant Human IL-1 beta Protein 10ng
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Anti-IL1 beta/IL1B Antibody Picoband®
IL1B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | WB |
Tested Applications
IL1B antibody was positively detected by WB in:
recombinant human IL-1 beta protein
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Rat, Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-IL1 beta/IL1B Antibody Picoband®
View all IL1B Antibodies
View all IL1B Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IL-1 beta.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated Molecular Weight
30.7 kDa
Background of IL1B
Interleukin-1 beta (IL-1beta) also know as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene, which localizes to the long arm of chromosome 2 at position 2q13-2q21 between two fragile sites. Interleukin 1 (IL-1) is a protein with several biological activities regulating host defense and immune responses. The human IL-1 family plays an important role in the pathogenesis of many diseases and functions as a key mediator of the host response to various infectious, inflammatory, and immunologic challenges.
Cite This Product
Anti-IL1 beta/IL1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1341)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
95%
High Homology
Dog
76%
Moderate Homology
Mouse
67%
Low homology
Rabbit
67%
Low homology
Rat
62%
Low homology
Cow
62%
Low homology
Sheep
62%
Low homology
Zebrafish
19%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for IL1B antibody
Publications
Application:
Species:
Cinnamaldehyde-surfactin nano-capsulation mitigates high-fat diet/ streptozotocin-induced diabetes and modulate gut microbial dysbiosis
Published: 2025-04-29
Journal: Food Bioscience
Application: WB
Impact Factor: 5.90
Species: Human
Alloferon Mitigates LPS-Induced Endometritis by Attenuating the NLRP3/CASP1/IL-1β/IL-18 Signaling Cascade
Published: 2024-06-24
Journal: INFLAMMATION
Application: WB
Impact Factor: 4.50
Species: Mouse
PMID: 38913143
Di-(2-ethylhexyl) phthalate aggravates psoriasis-like skin lesions: In vitro and in vivo evaluation
Published: 2023-10-01
Journal: TOXICOLOGY AND APPLIED PHARMACOLOGY
Application: WB
Impact Factor: 3.80
Species: Human
PMID: 37783235
Hyperglucose Contributes to Periodontitis: Involvement of the NLRP3 Pathway by Engaging the Innate Immunity of Oral Gingival Epithelium
Published: 2015-02-01
Journal: JOURNAL OF PERIODONTOLOGY
Application: IHC
Impact Factor: 2.71
Species: Human
PMID: 25325516
The effect of proinflammatory cytokines on IL-17RA expression in NSCLC
Published: 2014-08-12
Journal: MEDICAL ONCOLOGY
Application: IHC
Impact Factor: 2.06
Species: Human
PMID: 25112467
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Product Promise
We promise Anti-IL1 beta/IL1B Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PA1341
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026