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Product Info Summary
| SKU: | A00101-4 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IL-1 Beta/Il1b Antibody Picoband®
SKU/Catalog Number
A00101-4
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL-1 Beta/Il1b Antibody Picoband® catalog # A00101-4. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-IL-1 Beta/Il1b Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00101-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse IL-1 Beta/Il1b recombinant protein (Position: V118-S269).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00101-4 is reactive to Il1b in Mouse
Observed Molecular Weight
36 kDa
Calculated molecular weight
30.9 kDa
Background of Il1b
Interleukin-1β (IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1β, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Furthermore, Microenvironmental IL-1β and, to a lesser extent, IL-1α are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1β and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1β, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00101-4 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: mouse RAW2647 whole cell, mouse RAW2647 whole cell
Validation Images & Assay Conditions
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Western blot analysis of IL-1 Beta/Il1b using anti-IL-1 Beta/Il1b antibody (A00101-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse RAW264.7 whole cell lysates,
Lane 2: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-1 Beta/Il1b antigen affinity purified polyclonal antibody (Catalog # A00101-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-1 Beta/Il1b at approximately 36 kDa. The expected band size for IL-1 Beta/Il1b is at 31 kDa.
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Effects of OPN and OVE on activation of NF-ĸB pathways in LPS-stimulated RAW264.7 cells. Cells were pretreated with OVE or OPN at different concentrations for 1 h. Expression levels of p-NF-ĸB p65, NF-ĸB p65, p-IĸBα, and IĸBα were detected after 24 h of LPS treatment. (A) OPN treatment. (B) OVE treatment. All experiments were carried out in triplicates and data are presented as means ± SDs; one-way ANOVA analysis was adopted for multiple comparisons; ###P < 0.001, ####P < 0.0001, compared to the untreated control group; ****P < 0.0001, compared to the LPS control group.
Index in PubMed under a CC BY license. PMID: 39455284
Specific Publications For Anti-IL-1 Beta/Il1b Antibody Picoband® (A00101-4)
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