Anti-IL13 Antibody

Rabbit Polyclonal IL13 Antibody. Validated in WB and tested in Human.

SKU PA1701
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-IL13 Antibody
See all IL13 primary antibodies, ELISA kits and proteins
SKU/Catalog Number PA1701
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit Polyclonal IL13 Antibody. Validated in WB and tested in Human. Size: 100μg/vial.
Cite This Product Anti-IL13 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1701)
Specificity Anti-IL13 Antibody (PA1701) reacts with Human IL13, in native form and recombinant. Superfamily members of IL13 are not reactive to PA1701.
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Form Lyophilized
Immunogen Anti-IL13 Antibody (PA1701) was raised against A synthetic peptide corresponding to a sequence at the C-terminus of human IL-13(135-146aa HLKKLFREGRFN)..
Reactivity Human

Assay Details

Our Boster Quality Guarantee for Anti-IL13 Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Human

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-IL13 Antibody (PA1701).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

/antibody/pa1701 1 WB anti il 13 antibody.jpg

Anti-IL-13 antibody, PA1701, Western blotting
Lane 1: Recombinant Human IL-13 Protein 10ng
Lane 2: Recombinant Human IL-13 Protein 5ng
Lane 3: Recombinant Human IL-13 Protein 2.5ng

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P35225
Gene Name IL13
Protein Name Interleukin-13
Alternative Names Interleukin-13;IL-13;IL13;NC30;Show more
Subcellular Localization Secreted.
Molecular Weight 15816 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses.
Research Areas Cytokines, Immunology, Innate Immunity, Interleukins

*You can search these to find other products in these research areas.
Background IL-13, Interleukin 13, is a protein that in humans is encoded by the IL-13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2(Th2) cells, that is an important mediator of allergic inflammation and disease. The IL-13 gene is mapped to 5q23-q31. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor(IL-4Ralpha) and at least one of two known IL-13-specific binding chains. IL-13 acts more prominently as a molecular bridge linking allergic inflammatory cells to the non-immune cells in contact with them, thereby altering physiological function.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Publishing with Anti-IL13 Antibody (PA1701)? Please let us know so we can cite the reference in this product datasheet. Email us at support@bosterbio.com.

Order Product (PA1701)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1054 $240
100ug+Free Biotin Secondary BA1003 $240

USD $240

Ships next business day.

Troubleshooting

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Publications

Acute exposure of ozone induced pulmonary injury and the protective role of vitamin E through the Nrf2 pathway in Balb/c mice
Number 2 Feibi Recipe Reduces PM2.5-Induced Lung Injury in Rats
An IL-13 Promoter Polymorphism Associated with Liver Fibrosis in Patients with Schistosoma japonicum
CD68 and interleukin 13, prospective immune markers for esophageal squamous cell carcinoma prognosis prediction
Li J, Li L, Zuo H, Ke C, Yan B, Wen H, Zhang Y, Yang X. Plos One. 2014 Feb 3;9(2):E87887. Doi: 10.1371/Journal.Pone.0087887. Ecollection 2014. T-Helper Type-2 Contact Hypersensitivity Of Balb/C Mice Aggravated By Dibutyl Phthalate Via Long-Term De...
Wang B, Liang S, Wang Y, Zhu Xq, Gong W, Zhang Hq, Li Y, Xia Cm. Plos Negl Trop Dis. 2015 Jan 15;9(1):E0003434. Doi: 10.1371/Journal.Pntd.0003434. Ecollection 2015. Th17 Down-Regulation Is Involved In Reduced Progression Of Schistosomiasis Fibrosi...

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is il13ra2 antibody