Anti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband™

NHERF-1 antibody

Boster Bio Anti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband™ catalog # A02427-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A02427-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

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Product Name

Anti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband™

View all NHERF-1 Antibodies

SKU/Catalog Number

A02427-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband™ catalog # A02427-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02427-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human EBP50/NHERF-1/SLC9A3R1 recombinant protein (Position: K69-R338).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02427-1 is reactive to SLC9A3R1 in Human, Mouse, Rat

Applications

A02427-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Background of NHERF-1

Sodium-hydrogen antiporter 3 regulator 1 is a regulator of Sodium-hydrogen antiporter 3. It is encoded by the gene SLC9A3R1. It is mapped to 17q25.1. This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For SLC9A3R1 (Source: Uniprot.org, NCBI)

Uniprot ID

O14745

Gene ID

9368

Gene Name

SLC9A3R1

Full Name

Na(+)/H(+) exchange regulatory cofactor NHE-RF1

Weight

38.868kDa

Alternative Names

EBP50NHERF-1; ezrin-radixin-moesin binding phosphoprotein-50; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; NHERF1solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1; NHERFNPHLOP2; Regulatory cofactor of Na(+)/H(+) exchanger; sodium/hydrogen exchanger regulatory factor 1; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 1; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1; Solute car SLC9A3R1 EBP50, NHERF, NHERF-1, NHERF1, NPHLOP2 SLC9A3 regulator 1 Na(+)/H(+) exchange regulatory cofactor NHE-RF1|Na+/H+ exchange regulatory co-factor|ezrin-radixin-moesin binding phosphoprotein-50|regulatory cofactor of Na(+)/H(+) exchanger|solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SLC9A3R1, check out the SLC9A3R1 Infographic

SLC9A3R1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SLC9A3R1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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3 Customer Q&As for Anti-EBP50/NHERF-1/SLC9A3R1 Antibody Picoband™

Question

We are currently using anti-EBP50/NHERF-1/SLC9A3R1 antibody A02427-1 for rat tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-30

Answer

The anti-EBP50/NHERF-1/SLC9A3R1 antibody (A02427-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-30

Question

Do you have a BSA free version of anti-EBP50/NHERF-1/SLC9A3R1 antibody A02427-1 available?

K. Zhao

Verified customer

Asked: 2016-07-14

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-EBP50/NHERF-1/SLC9A3R1 antibody A02427-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-07-14

Question

I see that the anti-EBP50/NHERF-1/SLC9A3R1 antibody A02427-1 works with ELISA, what is the protocol used to produce the result images on the product page?

G. Wu

Verified customer

Asked: 2014-12-08

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2014-12-08

Size

Conjugation

Total: $315

SKU:A02427-1

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Ships directly to USA and Canada. For other regions, visit our distributors page

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Sample Inquiry

In stock
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A02427-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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