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Product Info Summary
| SKU: | PA1469 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Interleukin-13 IL13 Antibody Picoband®
SKU/Catalog Number
PA1469
BA1804-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interleukin-13 IL13 Antibody catalog # PA1469. Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interleukin-13 IL13 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1469)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of rat IL-13.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1469 is reactive to Il13 in Rat
Observed Molecular Weight
13 kDa
Calculated molecular weight
14.1 kDa
Background of Il13
IL-13, Interleukin 13, is a protein that in humans is encoded by the IL-13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells that is an important mediator of allergic inflammation and disease. The IL-13 gene is mapped to 5q23-q31. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Ralpha) and at least one of two known IL-13-specific binding chains. IL-13 acts more prominently as a molecular bridge linking allergic inflammatory cells to the non-immune cells in contact with them, thereby altering physiological function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1469 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat
Validation Images & Assay Conditions
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Anti-IL-13 antibody, PA1469, Western blotting
All lanes: Anti IL-13 (PA1469) at 0.5ug/ml
Lane 1: Recombinant Rat IL-13 Protein 5ng
Lane 2: Recombinant Rat IL-13 Protein 2.5ng
Predicted bind size: 13KD
Observed bind size: 13KD
Specific Publications For Anti-Interleukin-13 IL13 Antibody Picoband® (PA1469)
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3 Customer Q&As for Anti-Interleukin-13 IL13 Antibody Picoband®
Question
My team were happy with the WB result of your anti-IL13 antibody. However we have been able to see positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IL13 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
According to literature, blood does express IL13. Generally IL13 expresses in secreted. Regarding which tissues have IL13 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7721105
Peripheral blood lymphocyte, Pubmed ID: 1408833
Boster Scientific Support
Answered: 2019-10-04
Question
We have observed staining in rat left testis. Do you have any suggestions? Is anti-IL13 antibody supposed to stain left testis positively?
Verified Customer
Verified customer
Asked: 2018-07-06
Answer
Based on literature left testis does express IL13. Based on Uniprot.org, IL13 is expressed in left testis, blood, peripheral blood lymphocyte, among other tissues. Regarding which tissues have IL13 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7721105
Peripheral blood lymphocyte, Pubmed ID: 1408833
Boster Scientific Support
Answered: 2018-07-06
Question
We are currently using anti-IL13 antibody PA1469 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-22
Answer
The anti-IL13 antibody (PA1469) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-22
