Anti-Integrin alpha 2 ITGA2 Rabbit Monoclonal Antibody

Integrin alpha 2/CD49b antibody

Boster Bio Anti-Integrin alpha 2 ITGA2 Rabbit Monoclonal Antibody catalog # M01933. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01933
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

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Product Name

Anti-Integrin alpha 2 ITGA2 Rabbit Monoclonal Antibody

View all Integrin alpha 2/CD49b Antibodies

SKU/Catalog Number

M01933

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Integrin alpha 2 ITGA2 Rabbit Monoclonal Antibody catalog # M01933. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Integrin alpha 2 ITGA2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01933)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

GEB-9

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Integrin alpha 2

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01933 is reactive to ITGA2 in Human, Mouse, Rat

Applications

M01933 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

17 kDa

Calculated molecular weight

129.295kDa

Background of Integrin alpha 2/CD49b

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:100
FC 1:100

Validation Images & Assay Conditions

Gene/Protein Information For ITGA2 (Source: Uniprot.org, NCBI)

Gene Name

ITGA2

Full Name

Integrin alpha-2

Weight

129.295kDa

Superfamily

integrin alpha chain family

Alternative Names

alpha-2 subunit; CD49b antigen; CD49b; Integrin alpha 2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); ITGA2; VLA 2; VLA-2 alpha ITGA2 BR, CD49B, GPIa, HPA-5, VLA-2, VLAA2 integrin subunit alpha 2 integrin alpha-2|CD49 antigen-like family member B|alpha 2 subunit of VLA-2 receptor|collagen receptor|human platelet alloantigen system 5|integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)|platelet antigen Br|platelet glycoprotein GPIa|platelet membrane glycoprotein Ia|very late activation protein 2 receptor, alpha-2 subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ITGA2, check out the ITGA2 Infographic

ITGA2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ITGA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Integrin alpha 2 ITGA2 Rabbit Monoclonal Antibody

Question

Would anti-Integrin alpha 2 Rabbit Monoclonal antibody M01933 work on horse IP with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2020-01-01

Answer

Our lab technicians have not validated anti-Integrin alpha 2 Rabbit Monoclonal antibody M01933 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Integrin alpha 2 Rabbit Monoclonal antibody M01933 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse leukemic t-cell in IP, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-01

Question

We are currently using anti-Integrin alpha 2 Rabbit Monoclonal antibody M01933 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-12

Answer

The anti-Integrin alpha 2 Rabbit Monoclonal antibody (M01933) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-12

Question

I am interested in to test anti-Integrin alpha 2 Rabbit Monoclonal antibody M01933 on human leukemic t-cell for research purposes, then I may be interested in using anti-Integrin alpha 2 Rabbit Monoclonal antibody M01933 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-07-02

Answer

The products we sell, including anti-Integrin alpha 2 Rabbit Monoclonal antibody M01933, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-07-02

Question

I have a question about product M01933, anti-Integrin alpha 2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-07-02

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01933 anti-Integrin alpha 2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-07-02

Question

I was wanting to use your anti-Integrin alpha 2 Rabbit Monoclonal antibody for ICC for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?

J. Dhar

Verified customer

Asked: 2013-06-18

Answer

It shows on the product datasheet, M01933 anti-Integrin alpha 2 Rabbit Monoclonal antibody has been tested for Flow Cytometry, IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-06-18

Order DetailsPrice
M01933

100μL

$370
M01933-30ul

30μL sample

$99
M01933-carrier-free

Carrier Free

$370

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M01933
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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