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- Table of Contents
Lrig1 antibody (A04613-1) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of LRIG1 using anti-LRIG1 antibody (A04613-1).
LRIG1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LRIG1 Antibody (A04613-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
LRIG1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LRIG1 Antibody (A04613-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Anti-LRIG1 Antibody Picoband®
Lrig1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
Lrig1 antibody was positively detected by WB in:
human Caco-2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Lrig1 antibody was positively detected by IHC in:
human mammary cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-LRIG1 Antibody Picoband®
View all LRIG1 Antibodies
View all LRIG1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse LRIG1, which shares 90% amino acid (aa) sequence identity with human LRIG1.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
145 kDa
Calculated Molecular Weight
119.2 kDa
Background of Lrig1
Leucine-rich repeats and immunoglobulin-like domains protein 1 is a protein that in humans is encoded by the LRIG1 gene. It is mapped to 3p14.1. Leucine-rich repeats and immunoglobulin-like domains protein 1 is a protein that in humans is encoded by the LRIG1 gene. It encodes a transmembrane protein that has been shown to interact with receptor tyrosine kinases of the EGFR-family, MET and RET. This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.
Cite This Product
Anti-LRIG1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04613-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
95%
High Homology
Rabbit
93%
High Homology
Cow
93%
High Homology
Sheep
93%
High Homology
Human
90%
High Homology
Dog
90%
High Homology
Monkey
90%
High Homology
Drosophila
48%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Lrig1 antibody
Immunohistochemistry (IHC) protocol for Lrig1 antibody
Publications
Application:
Species:
The effect of airborne particulate matter 2.5 (PM2.5) on meibomian gland
Published: 2024-06-06
Journal: EXPERIMENTAL EYE RESEARCH
Application: IHC
Impact Factor: 3.00
Species: Mouse
PMID: 38849003
IGF2/IGF2R/Sting signaling as a therapeutic target in DSS-induced ulcerative colitis
Published: 2023-10-19
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 5.00
Species: Mouse
PMID: 37863414
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Product Promise
We promise Anti-LRIG1 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A04613-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026