Anti-LAMB3/Laminin Beta 3 Antibody

Laminin beta 3 antibody

Boster Bio Anti-LAMB3/Laminin Beta 3 Antibody (Catalog # A03310). Tested in IHC, IF applications. This antibody reacts with Human.

Product Info Summary

SKU: A03310
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: IF, IHC

Product Name

Anti-LAMB3/Laminin Beta 3 Antibody

View all Laminin beta 3 Antibodies

SKU/Catalog Number

A03310

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-LAMB3/Laminin Beta 3 Antibody (Catalog # A03310). Tested in IHC, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LAMB3/Laminin Beta 3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03310)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human LAMB3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03310 is reactive to LAMB3 in Human

Applications

A03310 is guaranteed for IF, IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

129.572kDa

Background of Laminin beta 3

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Gerecke D.R., J. Biol. Chem. 269:11073-11080(1994).
Morishima Y., ics 28:273-279(1995).
Robbins P.B., Proc. Natl. Acad. Sci. U.S.A. 98:5193-5198(2001).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For LAMB3 (Source: Uniprot.org, NCBI)

Gene Name

LAMB3

Full Name

Laminin subunit beta-3

Weight

129.572kDa

Alternative Names

BM600-125kDa; Epiligrin subunit bata; FLJ99565; Kalinin B1 chain; Kalinin subunit beta; kalinin-140kDa; LAM5; Laminin 5; Laminin B1k chain; laminin S B3 chain; laminin subunit beta-3; laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD)); laminin, beta 3; Laminin-5 subunit beta; LAMNB1; Nicein subunit beta; nicein-125kDa LAMB3 AI1A, BM600-125KDA, LAM5, LAMNB1 laminin subunit beta 3 laminin subunit beta-3|epiligrin subunit bata|kalinin B1 chain|kalinin subunit beta|kalinin-140kDa|laminin B1k chain|laminin S B3 chain|laminin beta 3|laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD))|laminin-5 subunit beta|nicein subunit beta|nicein-125kDa

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LAMB3, check out the LAMB3 Infographic

LAMB3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LAMB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-LAMB3/Laminin Beta 3 Antibody

Question

We are currently using anti-LAMB3/Laminin Beta 3 antibody A03310 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-05

Answer

The anti-LAMB3/Laminin Beta 3 antibody (A03310) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-05

Question

I see that the anti-LAMB3/Laminin Beta 3 antibody A03310 works with IHC, what is the protocol used to produce the result images on the product page?

R. Roberts

Verified customer

Asked: 2019-07-15

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-15

Question

Is this A03310 anti-LAMB3/Laminin Beta 3 antibody reactive to the isotypes of LAMB3?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

The immunogen of A03310 anti-LAMB3/Laminin Beta 3 antibody is Synthesized peptide derived from internal of human LAMB3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-05-21

Question

Does A03310 anti-LAMB3/Laminin Beta 3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-11-02

Answer

You can see on the product datasheet, A03310 anti-LAMB3/Laminin Beta 3 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-11-02

Order DetailsPrice
A03310-100uL

100uL

$360

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