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Product Info Summary
| SKU: | A00438S22 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Lamin A/C (phospho-S22) LMNA Antibody
SKU/Catalog Number
A00438S22
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Lamin A/C (phospho-S22) LMNA Antibody catalog # A00438S22. Tested in WB,IP applications. This antibody reacts with Human,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lamin A/C (phospho-S22) LMNA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00438S22)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
A synthetic phosphorylated peptide around S22 of human Lamin A(NP_005563.1).
Reactive Species
A00438S22 is reactive to LMNA in Human, Rat
Calculated molecular weight
74.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00438S22 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IP: 1:50-1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using Phospho-Lamin A/C-S22 antibody at 1:2000 dilution or Lamin A antibody . HeLa cells were treated by Hydroxyurea at 37℃ for 20 hours or treated by Paclitaxel at 37℃ for 20 hours. C6 cells were treated by Hydroxyurea at 37℃ for 20 hours or treated by Paclitaxel at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit .
Exposure time: 1s.
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Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-Lamin A/C-S22 pAb . Western blot was performed from the immunoprecipitate using Phospho-Lamin A/C-S22 pAb at a dilution of 1:1000. HeLa cells were treated by Paclitaxel at 37℃ for 20 hours.
Specific Publications For Anti-Lamin A/C (phospho-S22) LMNA Antibody (A00438S22)
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