Anti-Laminin alpha-3 LAMA3 Antibody

Product Name

Anti-Laminin alpha-3 LAMA3 Antibody

View all LAMA3 Antibodies

SKU/Catalog Number

A04194-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Laminin alpha-3 LAMA3 Antibody catalog # A04194-1. Tested in ELISA, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Laminin alpha-3 LAMA3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04194-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

The antiserum was produced against synthesized peptide derived from human LAMA3 at amino acid range 2571-2620

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04194-1 is reactive to LAMA3 in Human, Mouse, Rat

Applications

A04194-1 is guaranteed for ELISA, IF, IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

366.649kDa

Background of LAMA3

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L10E family of ribosomal proteins. It is located in the cytoplasm. In vitro studies have shown that the chicken protein can bind to c-Jun and can repress c-Jun-mediated transcriptional activation, but these activities have not been demonstrated in vivo. This gene was initially identified as a candidate for a Wilms tumor suppressor gene, but later studies determined that this gene is not involved in the suppression of Wilms tumor. This gene has been referred to as 'laminin receptor homolog' because a chimeric transcript consisting of sequence from this gene and sequence from the laminin receptor gene was isolated; however, it is not believed that this gene encodes a laminin receptor. Alternative splicing results in multiple transcript variants. This gene also uses multiple polyA signals, with the 3'-most polyA signal overlapping the deoxyribonuclease I-like 1 gene on the opposite strand. This gene is co-transcribed with the small nucleolar RNA gene U70, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Dowdy S.F., Nucleic Acids Res. 19:5763-5769(1991).
van den Ouweland A.M.W., Hum. Mol. Genet. 1:269-273(1992).
Kaneko K., Hum. Mol. Genet. 1:529-533(1992).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000

Validation Images & Assay Conditions

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using LAMA3 Antibody. The picture on the right is blocked with the synthesized peptide.

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Western blot analysis of the lysates from HeLa cells using BCLW antibody.

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Immunofluorescence analysis of HepG2 cells, using LAMA3 Antibody. The picture on the right is blocked with the synthesized peptide.

Gene/Protein Information For LAMA3 (Source: Uniprot.org, NCBI)

Alternative Names

Epiligrin; LAMA3; Laminin alpha 3; Laminin5; Laminin-5 LAMA3 BM600, E170, LAMNA, LOCS laminin subunit alpha 3 laminin subunit alpha-3|BM600 150kD subunit|LAM3, alpha-3 subunit|epiligrin 170 kda subunit|epiligrin alpha 3 subunit|kalinin 165kD subunit|kalinin subunit alpha|laminin 5, alpha-3 subunit|laminin A3|laminin, alpha 3 (nicein (150kD), kalinin (165kD), BM600 (150kD), epilegrin)|laminin-5 alpha 3 chain|laminin-5 subunit alpha|laminin-6 subunit alpha|laminin-7 subunit alpha|nicein 150kD subunit|nicein subunit alpha

For more info on LAMA3, check out the LAMA3 Infographic

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In this infographic, you will see the following information for LAMA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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