Anti-MAN1B1/Ermani Antibody

Product Name

Anti-MAN1B1/Ermani Antibody

View all ERManI Antibodies

SKU/Catalog Number

A06537

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MAN1B1/Ermani Antibody (Catalog # A06537). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MAN1B1/Ermani Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06537)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MAN1B1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A06537 is reactive to MAN1B1 in Human, Mouse

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Observed Molecular Weight

Calculated molecular weight

79580 MW

Background of ERManI

Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2, but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man5-6GlcNAc2.

Tremblay L.O., Glycobiology 9:1073-1078(1999) [PubMed: 10521544].
Gonzalez D.S., J. Biol. Chem. 274:21375-21386(1999) [PubMed: 10409699].
Clark H.F., Genome Res. 13:2265-2270(2003) [PubMed: 12975309].

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06537 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Positive Control

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of extracts from 3T3 cells, using MAN1B1 antibody A06537.

Gene/Protein Information For MAN1B1 (Source: Uniprot.org, NCBI)

Alternative Names

alpha 1,2-mannosidase; EC 3.2.1.113; endoplasmic reticulum alpha-mannosidase 1; endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase 1; endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase; ER alpha 1,2-mannosidase; ER alpha-1,2-mannosidase; ER ManI; ER mannosidase 1; ERMan1; MAN1B1; Man9GlcNAc2-specific processing alpha-mannosidase; Man9GlcNAc2-specific-processing alpha-mannosidase; MANA-ER; Mannosidase alpha class 1B member 1; mannosidase, alpha, class 1B, member 1; MRT15; RP11-350O14.2 MAN1B1 ERMAN1, ERManI, MANA-ER, MRT15 mannosidase alpha class 1B member 1 endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase|ER alpha 1,2-mannosidase|Man9GlcNAc2-specific processing alpha-mannosidase|endoplasmic Reticulum Class I alpha-mannosidase|endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase 1

For more info on MAN1B1, check out the MAN1B1 Infographic

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In this infographic, you will see the following information for MAN1B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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