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Product Info Summary
| SKU: | PA1357 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Matrix metalloproteinase-9 MMP9 Antibody Picoband®
SKU/Catalog Number
PA1357
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Matrix metalloproteinase-9 MMP9 Antibody catalog # PA1357. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Matrix metalloproteinase-9 MMP9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1357)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MMP-9, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1357 is reactive to MMP9 in Human
Observed Molecular Weight
60 kDa
Calculated molecular weight
78.5 kDa
Background of MMP9
Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is an enzyme that in humans is encoded by the MMP9 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1357 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: Rat Embryo Tissue, MM453 Cell, HELA Cell, SMMC Cell, JURKAT Cell, HT1080 Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-MMP-9 antibody, PA1357, Western blotting
Lane 1: Rat Embryo Tissue Lysate
Lane 2: MM453 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: SMMC Cell Lysate
Lane 5: JURKAT Cell Lysate
Lane 6: HT1080 Cell Lysate
Specific Publications For Anti-Matrix metalloproteinase-9 MMP9 Antibody Picoband® (PA1357)
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Customer Q&As
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16 Customer Q&As for Anti-Matrix metalloproteinase-9 MMP9 Antibody Picoband®
Question
Would PA1357 anti-MMP9 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
It shows on the product datasheet, PA1357 anti-MMP9 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-08
Question
Can you help my question with product PA1357, anti-MMP9 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1357 anti-MMP9 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-23
Question
We have been able to see staining in human neutrophil. Do you have any suggestions? Is anti-MMP9 antibody supposed to stain neutrophil positively?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
Based on literature neutrophil does express MMP9. Based on Uniprot.org, MMP9 is expressed in tibia, umbilical cord blood, b-cell, neutrophil, fibrosarcoma, blood, monocytic leukemia, among other tissues. Regarding which tissues have MMP9 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Blood, Pubmed ID: 1480034, 1281792
Fibrosarcoma, Pubmed ID: 1400481, 7669817
Monocytic leukemia, Pubmed ID: 20800577
Neutrophil, Pubmed ID: 1645657, 1464361, 1653055
Umbilical cord blood, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-04-02
Question
Is this PA1357 anti-MMP9 antibody reactive to the isotypes of MMP9?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
The immunogen of PA1357 anti-MMP9 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human MMP-9(689-705aa NQVDQVGYVTYDILQCP), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-23
Question
My team were happy with the WB result of your anti-MMP9 antibody. However we have observed positive staining in neutrophil extracellular space using this antibody. Is that expected? Could you tell me where is MMP9 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
Based on literature, neutrophil does express MMP9. Generally MMP9 expresses in secreted, extracellular space, extracellular. Regarding which tissues have MMP9 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Blood, Pubmed ID: 1480034, 1281792
Fibrosarcoma, Pubmed ID: 1400481, 7669817
Monocytic leukemia, Pubmed ID: 20800577
Neutrophil, Pubmed ID: 1645657, 1464361, 1653055
Umbilical cord blood, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-03-18
Question
Is a blocking peptide available for product anti-MMP9 antibody (PA1357)?
R. Yang
Verified customer
Asked: 2020-03-16
Answer
We do provide the blocking peptide for product anti-MMP9 antibody (PA1357). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-16
Question
Will anti-MMP9 antibody PA1357 work for WB with neutrophil?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
According to the expression profile of neutrophil, MMP9 is highly expressed in neutrophil. So, it is likely that anti-MMP9 antibody PA1357 will work for WB with neutrophil.
Boster Scientific Support
Answered: 2020-01-31
Question
Is there a BSA free version of anti-MMP9 antibody PA1357 available?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MMP9 antibody PA1357 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-08
Question
I see that the anti-MMP9 antibody PA1357 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-06-03
Question
Does anti-MMP9 antibody PA1357 work on canine WB with blood?
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
Our lab technicians have not tested anti-MMP9 antibody PA1357 on canine. You can run a BLAST between canine and the immunogen sequence of anti-MMP9 antibody PA1357 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-06
Question
I was wanting to use to test anti-MMP9 antibody PA1357 on human neutrophil for research purposes, then I may be interested in using anti-MMP9 antibody PA1357 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
The products we sell, including anti-MMP9 antibody PA1357, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-26
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for neutrophil using anti-MMP9 antibody PA1357. Let me know if you need anything else.
K. Johnson
Verified customer
Asked: 2017-12-21
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-21
Question
I have attached the WB image, lot number and protocol we used for neutrophil using anti-MMP9 antibody PA1357. Please let me know if you require anything else.
M. Carter
Verified customer
Asked: 2017-12-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-11
Question
We are currently using anti-MMP9 antibody PA1357 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
The anti-MMP9 antibody (PA1357) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-26
Question
I would like using your anti-MMP9 antibody for interleukin-4 and interleukin-13 signaling studies. Has this antibody been tested with western blotting on mm453 cell lysate? We would like to see some validation images before ordering.
D. Anderson
Verified customer
Asked: 2014-10-23
Answer
We appreciate your inquiry. This PA1357 anti-MMP9 antibody is validated on rat embryo tissue lysate, mm453 cell lysate, hela cell lysate, smmc cell lysate, jurkat cell lysate, ht1080 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-10-23
Question
I was wanting to use your anti-MMP9 antibody for WB for human neutrophil on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human neutrophil identification?
S. Dhar
Verified customer
Asked: 2014-07-24
Answer
You can see on the product datasheet, PA1357 anti-MMP9 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human neutrophil in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-07-24
