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Product Info Summary
| SKU: | PA1382 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-RANK/TNFRSF11A Antibody Picoband®
SKU/Catalog Number
PA1382
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RANK/TNFRSF11A Antibody catalog # PA1382. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RANK/TNFRSF11A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1382)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human RANK.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1382 is reactive to TNFRSF11A in Human
Observed Molecular Weight
90 kDa
Calculated molecular weight
66.0 kDa
Background of TNFRSF11A
Receptor Activator of Nuclear Factor K B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is also expressed on dendritic cells and facilitates immune signaling. RANKL (Receptor Activator for Nuclear Factor K B Ligand) is found on the surface of stromal cells, osteoblasts, and T cells. By analysis of somatic cell and radiation hybrid panels, Anderson et al. (1997) mapped the RANK gene to 18q22.1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1382 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human
Positive Control
IHC: Human Mammary Cancer Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-RANK antibody, PA1382, Western blotting
Lane 1: Recombinant Human RANK Protein 10ng
Lane 2: Recombinant Human RANK Protein 5ng
Lane 3: Recombinant Human RANK Protein 2.5ng
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Anti-RANK antibody, PA1382, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Specific Publications For Anti-RANK/TNFRSF11A Antibody Picoband® (PA1382)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-RANK/TNFRSF11A Antibody Picoband®
Question
My lab would like to test anti-RANK/TNFRSF11A antibody PA1382 on human cervix carcinoma for research purposes, then I may be interested in using anti-RANK/TNFRSF11A antibody PA1382 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
The products we sell, including anti-RANK/TNFRSF11A antibody PA1382, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-RANK/TNFRSF11A antibody PA1382. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-21
Question
Will anti-RANK/TNFRSF11A antibody PA1382 work on horse WB with blood?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
Our lab technicians have not validated anti-RANK/TNFRSF11A antibody PA1382 on horse. You can run a BLAST between horse and the immunogen sequence of anti-RANK/TNFRSF11A antibody PA1382 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-20
Question
Does PA1382 anti-RANK/TNFRSF11A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
F. Taylor
Verified customer
Asked: 2017-08-11
Answer
As indicated on the product datasheet, PA1382 anti-RANK/TNFRSF11A antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-11
Question
Is there a BSA free version of anti-RANK/TNFRSF11A antibody PA1382 available?
Verified Customer
Verified customer
Asked: 2017-07-10
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-RANK/TNFRSF11A antibody PA1382 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-10
