Rat MMP-9 ELISA Kit PicoKine®

MMP-9 ELISA kit for Rat

Rat MMP-9 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Mmp9 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 15pg/ml. Cited in 23 publication(s).

Product Info Summary

SKU: EK1463
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Rat MMP-9 ELISA Kit PicoKine®

View all MMP-9 ELISA kits

SKU/Catalog Number

EK1463

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat MMP-9 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Mmp9 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 15pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat MMP-9 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1463)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L35-P708

Sensitivity

<15 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1463 is reactive to Mmp9 in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK1463 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of MMP-9

Matrix metallopeptidase 9 (MMP-9) is also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.

Kit Components

Catalog Number Description Quantity
EK1463-CAP Anti-Rat Mmp9 Pre-coated 96-well strip microplate 1
EK1463-ST Rat Mmp9 Standard 2 vials, 20 ng/tube
EK1463-DA Rat Mmp9 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0470.1150.1950.3210.6151.0011.6352.065

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect MMP-9, Dilution ratio of 1:5, concentration in serum is around 20ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat MMP-9 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)4292362888045021998392
Standard deviation29.17153.53594.9633.75167.12646.18
CV (%)6.8%6.5%6.7%7.5%7.6%7.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MMP-9 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 142945742044943814.873.3%
Sample 22362245323322307236355.222.3%
Sample 3888010067901394349348462.494.9%
*number of samples for each test n=16.

Gene/Protein Information For Mmp9 (Source: Uniprot.Org, NCBI)

Uniprot ID

P50282

Gene ID

81687

Gene Name

Mmp9

Full Name

Matrix metalloproteinase-9

Weight

78.611kDa

Superfamily

peptidase M10A family

Alternative Names

92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase Mmp9|matrix metallopeptidase 9|matrix metalloproteinase-9|92 kDa gelatinase|92-kDa type IV collagenase|GELB|MMP-9|gelatinase B|matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Mmp9, check out the Mmp9 Infographic

Mmp9 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Mmp9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1463 has been cited in 23 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Calcium‑sensing receptor promotes high glucose‑induced myocardial fibrosis via upregulation of the TGF‑β1/Smads pathway in cardiac fibroblasts

Liposomal Curcumin is Better than Curcumin to Alleviate Complications in Experimental Diabetic Mellitus

Association of NF-κB and AP-1 with MMP-9 Overexpression in 2-Chloroethanol Exposed Rat Astrocytes

Thrombin Aggravates Hypoxia/Reoxygenation Injury of Cardiomyocytes by Activating an Autophagy Pathway-Mediated by SIRT1

Attenuation of Cardiac Autonomic Neuropathy by Escin in Diabetic Rats

Exposure to Air Pollution Exacerbates Inflammation in Rats with Preexisting COPD

Effects of Curcumin Nanoparticles in Isoproterenol-Induced Myocardial Infarction

Synovial fibroblast‐targeting liposomes encapsulating an NF‐κB‐blocking peptide ameliorates zymosan‐induced synovial inflammation

Attenuation of lipid metabolic abnormalities, proinflammatory cytokines, and matrix metalloproteinase expression by biochanin-A in isoproterenol-induced myocardial infarction in rats

Effective-components combination improves airway remodeling in COPD rats by suppressing M2 macrophage polarization via the inhibition of mTORC2 activity

Effect of crocetin on vascular smooth muscle cells migration induced by advanced glycosylation end products

Effective-component compatibility of Bufei Yishen formula II inhibits mucus hypersecretion of chronic obstructive pulmonary disease rats by regulating EGFR/PI3K/mTOR signaling

Polysaccharide from Ephedra sinica Stapf inhibits inflammation expression by regulating Factor-β1/Smad2 signaling

Catechin attenuates diabetic autonomic neuropathy in streptozotocin induced diabetic rats

Luteolin inhibits IL-1β-induced inflammation in rat chondrocytes and attenuates osteoarthritis progression in a rat model

Baicalin Reduces Early Brain Injury after Subarachnoid Hemorrhage in Rats

Bulboacă AE,Porfire A,Bolboacă SD,Nicula CA,Feștilă DG,Roman A,Râjnoveanu RM,Râjnoveanu A,Dogaru G,Boarescu PM,Rus V, Bulboacă CA,Bulboacă AI,Stănescu I.Protective Effects of Liposomal Curcumin on Oxidative Stress/Antioxidant Imbalance, Metalloproteinases
Species: Rat
EK1463 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Li J,Xie Y,Zhao P,Qin Y,Oliver BG,Tian Y,Li S,Wang M,Liu X. A chinese herbal formula ameliorates COPD by inhibiting the inflammatory response via downregulation of p65, JNK, and p38. Phytomedicine. 2021 Jan 20;83:153475.doi:10.1016/j.phymed.2021.153475.Ep
Species: Human,Rat
EK1463 usage in article: APP:ELISA, SAMPLE:SERUM AND BALF, DILUTION:NA

Li J,Ma J,Tian Y,Zhao P,Liu X,Dong H,Zheng W,Feng S,Zhang L,Wu M,Zhu L,Liu S,Zhao D.Effective-component compatibility of Bufei Yishen formula II inhibits mucus hypersecretion of chronic obstructive pulmonary disease rats by regulating EGFR/PI3K/mTOR signa
Species: Rat
EK1463 usage in article: APP:ELISA, SAMPLE:SERUM AND BALF, DILUTION:NA

Association of NF-%u03BAB and AP-1 with MMP-9 Overexpression in 2-Chloroethanol Exposed Rat Astrocytes

The Angiogenic Effect of microRNA-21 Targeting TIMP3 through the Regulation of MMP2 and MMP9

You C, Zu J, Liu X, Kong P, Song C, Wei R, Zhou C, Wang Y, Yan J. J Cell Mol Med. 2018 Apr;22(4):2449-2457. doi: 10.1111/jcmm.13549. Epub 2018 Jan 30. Synovial fibroblast‐targeting liposomes encapsulating an NF‐κB‐blocking peptide ameliorates zymo...

Tang Xp, Tang Gd, Fang Cy, Liang Zh, Zhang Ly. World J Gastroenterol. 2013 Mar 14;19(10):1582-92. Doi: 10.3748/Wjg.V19.I10.1582. Effects Of Ginsenoside Rh2 On Growth And Migration Of Pancreatic Cancer Cells.

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9 Customer Q&As for Rat MMP-9 ELISA Kit PicoKine®

Question

Q: Can MMP-9 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

W. Singh

Verified customer

Asked: 2020-03-01

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-03-01

Question

I wanted to know that MMP-9 is your Rat MMP-9 PicoKine ELISA specific for. The website states natural and recombinant MMP-9 but is it the pro, inactive, or TIMP-complexed form of MMP-9???

Verified Customer

Verified customer

Asked: 2020-02-21

Answer

EK1463 recognizes total form of MMP-9, including

Boster Scientific Support

Answered: 2020-02-21

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

W. Walker

Verified customer

Asked: 2019-11-24

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-11-24

Question

Q: if the enzyme conjugated MMP-9 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MMP-9 antigen?

Verified Customer

Verified customer

Asked: 2019-02-17

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-02-17

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

K. Yang

Verified customer

Asked: 2019-01-25

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-01-25

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis MMP-9 using the EK1463 Rat MMP-9 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-12-02

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2017-12-02

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

K. Varma

Verified customer

Asked: 2017-05-30

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-05-30

Question

Q: how can I thaw whole blood sample for MMP-9 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2016-08-22

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MMP-9 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2016-08-22

Question

What form of MMP-9 does this kit recognize?

Verified Customer

Verified customer

Asked: 2013-03-27

Answer

EK1463 recognizes total form of MMP-9, including the pro, inactive, or TIMP-complexed form of MMP-9.

Boster Scientific Support

Answered: 2013-03-27

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