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- Table of Contents
Vcam1 antibody (A01199-2) performs well in ELISA, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199-2).
VCAM1 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCAM1 Antibody (A01199-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
VCAM1 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCAM1 Antibody (A01199-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Anti-VCAM1 Antibody Picoband®
Vcam1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, IHC, WB |
Tested Applications
Vcam1 antibody was positively detected by WB in:
rat spleen tissue, rat thymus tissue, rat testis tissue, rat kidney tissue, rat lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Vcam1 antibody was positively detected by IHC in:
rat kidney tissue, rat spleen tissue, mouse spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Product Information
Product Name
Anti-VCAM1 Antibody Picoband®
View all VCAM1 Antibodies
View all VCAM1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat VCAM1 recombinant protein (Position: F25-L270).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
110 kDa
Calculated Molecular Weight
81.2 kDa
Background of Vcam1
CD106 (cluster of differentiation 106) also known as vascular cell adhesion molecule 1 (VCAM-1), is a protein that in humans is encoded by the VCAM1 gene. In inflammatory conditions and in cardiac allografts undergoing rejection, VCAM1 is upregulated in endothelium of postcapillary venules. Arterial expression of VCAM1 is also found in experimental models of atherosclerosis in the rabbit. This gene is mapped to chromosome 1 by Southern analysis of somatic cell hybrids. VCAM-1 functions as a cell adhesion molecule. The VCAM-1 protein mediates the adhesion of lymphocytes, monocytes, eosinophils, andbasophils to vascularendothelium. It also functions in leukocyte-endothelial cell signal transduction, and it may play a role in the development ofatherosclerosis and rheumatoid arthritis. CAM741 works similar to cotransin in that it represses the biosynthesis of VCAM1 cells by blocking the process of cotranslational translocation, which is dependent on the signal peptide ofVCAM1. Among the lung metastasis signature genes identified, several, includingVCAM1, were functionally validated.
Cite This Product
Anti-VCAM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01199-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Rat
100%
High Homology
Mouse
84%
Moderate Homology
Rabbit
77%
Moderate Homology
Monkey
77%
Moderate Homology
Human
75%
Moderate Homology
Dog
73%
Moderate Homology
Sheep
69%
Low homology
Cow
68%
Low homology
Zebrafish
30%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Vcam1 antibody
Immunohistochemistry (IHC) protocol for Vcam1 antibody
Publications
Application:
Species:
Sex differences in kidney and lung status in an animal model of brain death
Published: 2025-03-26
Journal: Clinics
Application: IHC
Impact Factor: 2.40
Species: Rat
PMID: 40147183
Characterization of a novel polysaccharide from Moutan Cortex and its ameliorative effect on AGEs-induced diabetic nephropathy
Published: 2021-02-10
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB
Impact Factor: 6.95
Species: Rat
PMID: 33581205
Identification of Active Compounds From Yi Nationality Herbal Formula Wosi Influencing COX-2 and VCAM-1 Signaling
Published: 2020-11-09
Journal: Frontiers in Pharmacology
Application: IHC
Impact Factor: 4.23
Species: Rat
PMID: 33442381
High-density lipoprotein of patients with breast cancer complicated with type 2 diabetes mellitus promotes cancer cells adhesion to vascular endothelium via ICAM-1 and VCAM-1 upregulation
Published: 2016-02-12
Journal: BREAST CANCER RESEARCH AND TREATMENT
Application: IHC
Impact Factor: 4.09
Species: Mouse
PMID: 26872904
Heme oxygenase-1 alleviates cigarette smoke-induced restenosis after vascular angioplasty by attenuating inflammation in rat model
Published: 2016-01-22
Journal: TOXICOLOGY LETTERS
Application: WB
Impact Factor: 3.52
Species: Rat
PMID: 26809138
The protective effect of melatonin on smoke-induced vascular injury in rats and humans: a randomized controlled trial
Published: 2015-12-17
Journal: JOURNAL OF PINEAL RESEARCH
Application: WB
Impact Factor: 9.60
Species: Rat
PMID: 26681403
Protective effect of melatonin on cigarette smoke-induced restenosis in rat carotid arteries after balloon injury
Published: 2014-09-24
Journal: JOURNAL OF PINEAL RESEARCH
Application: WB
Impact Factor: 7.81
Species: Rat
PMID: 25251422
The Neurovascular Protective Effects of Huperzine A on <smlcap>D</smlcap>-Galactose-Induced Inflammatory Damage in the Rat Hippocampus
Published: 2014-08-27
Journal: GERONTOLOGY
Application: WB
Impact Factor: 2.68
Species: Rat
PMID: 24969491
IL-37 Ameliorates the Inflammatory Process in Psoriasis by Suppressing Proinflammatory Cytokine Production
Published: 2014-02-15
Journal: JOURNAL OF IMMUNOLOGY
Application: IHC
Impact Factor: 5.36
Species: Mouse
PMID: 24453242
Simvastatin suppresses vascular inflammation and atherosclerosis in ApoE−/− mice by downregulating the HMGB1-RAGE axis
Published: 2013-04-08
Journal: ACTA PHARMACOLOGICA SINICA
Application: WB
Impact Factor: 2.35
Species: Mouse,Human
PMID: 23564080
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Product Promise
We promise Anti-VCAM1 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01199-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026