Anti-VCAM1 Antibody Picoband®

Western blot analysis of VCAM1 using anti-VCAM1 antibody (A01199-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat testis tissue lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: rat lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCAM1 antigen affinity purified polyclonal antibody (Catalog # A01199-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCAM1 at approximately 110KD. The expected band size for VCAM1 is at 81KD.

Effect of Wosi and colchicine treatment on the level of (A) COX-2 and (C) VCAM-1 proteins were detected by immunohistochemistry (IHC). Representative photomicrographs of staining (×20) demonstrating the synovial tissue sections from ankle joints of experiments rats. Treatment of groups are as follow: (a) control group; (b) model group; (c) colchicine group (0.2 mg/kg); (d) Wosi group (0.5 g/kg); (e) Wosi group (1 g/kg); (f) Wosi group (2 g/kg). The integrated optical density (IOD) of COX-2 (B) and VCAM-1 (D) IHC-stained materials were analyzed utilizing the Image pro plus 7.0 software. IOD = optical intensity of positive cells × area of positive cells. The value was expressed as mean ± SEM for ten animals. ###p < 0.001 vs. control group; **p < 0.01; ***p < 0.001 vs. model group.
Index in PubMed under a CC BY license. PMID: 33442381

IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199-2).
VCAM1 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCAM1 Antibody (A01199-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199-2).
VCAM1 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCAM1 Antibody (A01199-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199-2).
VCAM1 was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCAM1 Antibody (A01199-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.